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Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus

Comparison of bacterial communities in T. putrescentiae populations based on Sanger sequencing of the 16S rRNA gene clones, the amplicons originated from eubacterial primers (F24/R1492): (A,B) principal coordinate analyses of bacterial communities in examined mite populations; (A) based on Euclidian distance; (B) based on Jacquard similarity index; (C) relative proportions of cloned bacterial sequences in our 16S rRNA library from different populations of T. putrescentiae. Abbreviations for the T. putrescentiae populations are listed in Table 1.
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Figure 6: Comparison of bacterial communities in T. putrescentiae populations based on Sanger sequencing of the 16S rRNA gene clones, the amplicons originated from eubacterial primers (F24/R1492): (A,B) principal coordinate analyses of bacterial communities in examined mite populations; (A) based on Euclidian distance; (B) based on Jacquard similarity index; (C) relative proportions of cloned bacterial sequences in our 16S rRNA library from different populations of T. putrescentiae. Abbreviations for the T. putrescentiae populations are listed in Table 1.

Mentions: The populations differed in observed bacterial community as indicated by the 16S rRNA library. Principal coordinate analyses using Euclidian data matrix showed that bacterial communities of Phillips, and Dog mite populations were similar, while Zvoleneves, Laboratory and Ham populations were formed from different bacteria (Figure 6A). The first principal axis explained 60% and the second axis explained 23% of variation in the dataset. When Jaccard data matrix was calculated, the bacterial community was similar in Zvoleneves, Dog and Phillips populations, while Laboratory and Ham populations were different (Figure 6B). The first axis explained 59% and second axis explained 29% of variation in the data set. Composition between populations is reported in Figure 6C.


Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Comparison of bacterial communities in T. putrescentiae populations based on Sanger sequencing of the 16S rRNA gene clones, the amplicons originated from eubacterial primers (F24/R1492): (A,B) principal coordinate analyses of bacterial communities in examined mite populations; (A) based on Euclidian distance; (B) based on Jacquard similarity index; (C) relative proportions of cloned bacterial sequences in our 16S rRNA library from different populations of T. putrescentiae. Abbreviations for the T. putrescentiae populations are listed in Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940368&req=5

Figure 6: Comparison of bacterial communities in T. putrescentiae populations based on Sanger sequencing of the 16S rRNA gene clones, the amplicons originated from eubacterial primers (F24/R1492): (A,B) principal coordinate analyses of bacterial communities in examined mite populations; (A) based on Euclidian distance; (B) based on Jacquard similarity index; (C) relative proportions of cloned bacterial sequences in our 16S rRNA library from different populations of T. putrescentiae. Abbreviations for the T. putrescentiae populations are listed in Table 1.
Mentions: The populations differed in observed bacterial community as indicated by the 16S rRNA library. Principal coordinate analyses using Euclidian data matrix showed that bacterial communities of Phillips, and Dog mite populations were similar, while Zvoleneves, Laboratory and Ham populations were formed from different bacteria (Figure 6A). The first principal axis explained 60% and the second axis explained 23% of variation in the dataset. When Jaccard data matrix was calculated, the bacterial community was similar in Zvoleneves, Dog and Phillips populations, while Laboratory and Ham populations were different (Figure 6B). The first axis explained 59% and second axis explained 29% of variation in the data set. Composition between populations is reported in Figure 6C.

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus