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Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus

The diversity and composition of bacterial communities in T. putrescentiae populations based on Sanger sequencing of the 16S rRNA gene clones, the amplicons originated from eubacterial primers (F24/R1492): (A) rarefaction analyses, (B) inverse Simpson diversity index, (C) heat map. Abbreviations for the T. putrescentiae populations are listed in Table 1.
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Figure 5: The diversity and composition of bacterial communities in T. putrescentiae populations based on Sanger sequencing of the 16S rRNA gene clones, the amplicons originated from eubacterial primers (F24/R1492): (A) rarefaction analyses, (B) inverse Simpson diversity index, (C) heat map. Abbreviations for the T. putrescentiae populations are listed in Table 1.

Mentions: Obtained 16S rRNA sequences were deposited in GenBank (Accession Numbers: KX022128–KX022390) and combined to 176 sequences of the laboratory T. putrescentiae population which are available in GenBank (Accession Numbers: JN236405–JN236431; JX001234–JX001344, KJ635082–KJ635148; Hubert et al., 2012a,b; Kopecky et al., 2014b). All the sequences originated from amplification and cloning the amplicons from universal eubacterial primers. Altogether 42 OTUs97 were distinguished (Supplementary Table S1). The diversity of bacterial community was similar for all populations of T. putrescentiae with exception of Zvoleneves population (Figures 5A,B). The bacterial community of mite population was formed from known/suspected symbionts or parasites: Bartonella-like bacteria (OTU97 1), Wolbachia (OTU97 4), Cardinium (OTU97 6), Blattabacterium-like symbiont (OTU97 7), Solitalea-like bacteria (OTU97 8; Figure 5C). The next most frequent and abundant OTUs97 were Bacillus (OTU97 2), Moraxella (OTU97 3), Staphylococcus (OTU97 5), Kocuria (OTU97 9), and Microbacterium (OTU97 10; Supplementary Table S1).


Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

The diversity and composition of bacterial communities in T. putrescentiae populations based on Sanger sequencing of the 16S rRNA gene clones, the amplicons originated from eubacterial primers (F24/R1492): (A) rarefaction analyses, (B) inverse Simpson diversity index, (C) heat map. Abbreviations for the T. putrescentiae populations are listed in Table 1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940368&req=5

Figure 5: The diversity and composition of bacterial communities in T. putrescentiae populations based on Sanger sequencing of the 16S rRNA gene clones, the amplicons originated from eubacterial primers (F24/R1492): (A) rarefaction analyses, (B) inverse Simpson diversity index, (C) heat map. Abbreviations for the T. putrescentiae populations are listed in Table 1.
Mentions: Obtained 16S rRNA sequences were deposited in GenBank (Accession Numbers: KX022128–KX022390) and combined to 176 sequences of the laboratory T. putrescentiae population which are available in GenBank (Accession Numbers: JN236405–JN236431; JX001234–JX001344, KJ635082–KJ635148; Hubert et al., 2012a,b; Kopecky et al., 2014b). All the sequences originated from amplification and cloning the amplicons from universal eubacterial primers. Altogether 42 OTUs97 were distinguished (Supplementary Table S1). The diversity of bacterial community was similar for all populations of T. putrescentiae with exception of Zvoleneves population (Figures 5A,B). The bacterial community of mite population was formed from known/suspected symbionts or parasites: Bartonella-like bacteria (OTU97 1), Wolbachia (OTU97 4), Cardinium (OTU97 6), Blattabacterium-like symbiont (OTU97 7), Solitalea-like bacteria (OTU97 8; Figure 5C). The next most frequent and abundant OTUs97 were Bacillus (OTU97 2), Moraxella (OTU97 3), Staphylococcus (OTU97 5), Kocuria (OTU97 9), and Microbacterium (OTU97 10; Supplementary Table S1).

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus