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Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of the Blattabacterium-like symbiont clones obtained from the Dog population of T. putrescentiae. The phylogeny was inferred by a Bayesian analysis of 24 partial 16S rRNA gene sequences with the reference sequences from RDP, i.e., 126 sequences of Blattabacterium, C. Brownia, and C. Sulcia, and the sequences of 398 type strains representing the order Flavobacteriales. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.5). Branch labels indicate Bayesian posterior probabilities and bootstrap support values from maximum likelihood analysis. The phylogram was rooted using Bacteroides fragilis sequence NR074784 as an outgroup.
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Figure 3: Phylogenetic analysis of the Blattabacterium-like symbiont clones obtained from the Dog population of T. putrescentiae. The phylogeny was inferred by a Bayesian analysis of 24 partial 16S rRNA gene sequences with the reference sequences from RDP, i.e., 126 sequences of Blattabacterium, C. Brownia, and C. Sulcia, and the sequences of 398 type strains representing the order Flavobacteriales. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.5). Branch labels indicate Bayesian posterior probabilities and bootstrap support values from maximum likelihood analysis. The phylogram was rooted using Bacteroides fragilis sequence NR074784 as an outgroup.

Mentions: The Blattabacterium symbiont was amplified using specific primers designed for identification of Blattabacterium in cockroaches. The symbiont was present in mite populations from dog food only as indicated by 16S rRNA sequences from eubacterial primers, as well as the amplicons of 16S rRNA fragments obtained by specific Blattabacterium primers. A Bayesian analysis of the obtained sequences showed that the sequences clustered as a monophyletic lineage outside Blattabacterium (Clark and Kambhampati, 2003), Candidatus Brownia rhizoecola (Gruwell et al., 2010), C. Uzinora diaspidicola (Gruwell et al., 2007), and C. Sulcia muelleri (Moran et al., 2005; Figure 3).


Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Phylogenetic analysis of the Blattabacterium-like symbiont clones obtained from the Dog population of T. putrescentiae. The phylogeny was inferred by a Bayesian analysis of 24 partial 16S rRNA gene sequences with the reference sequences from RDP, i.e., 126 sequences of Blattabacterium, C. Brownia, and C. Sulcia, and the sequences of 398 type strains representing the order Flavobacteriales. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.5). Branch labels indicate Bayesian posterior probabilities and bootstrap support values from maximum likelihood analysis. The phylogram was rooted using Bacteroides fragilis sequence NR074784 as an outgroup.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940368&req=5

Figure 3: Phylogenetic analysis of the Blattabacterium-like symbiont clones obtained from the Dog population of T. putrescentiae. The phylogeny was inferred by a Bayesian analysis of 24 partial 16S rRNA gene sequences with the reference sequences from RDP, i.e., 126 sequences of Blattabacterium, C. Brownia, and C. Sulcia, and the sequences of 398 type strains representing the order Flavobacteriales. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.5). Branch labels indicate Bayesian posterior probabilities and bootstrap support values from maximum likelihood analysis. The phylogram was rooted using Bacteroides fragilis sequence NR074784 as an outgroup.
Mentions: The Blattabacterium symbiont was amplified using specific primers designed for identification of Blattabacterium in cockroaches. The symbiont was present in mite populations from dog food only as indicated by 16S rRNA sequences from eubacterial primers, as well as the amplicons of 16S rRNA fragments obtained by specific Blattabacterium primers. A Bayesian analysis of the obtained sequences showed that the sequences clustered as a monophyletic lineage outside Blattabacterium (Clark and Kambhampati, 2003), Candidatus Brownia rhizoecola (Gruwell et al., 2010), C. Uzinora diaspidicola (Gruwell et al., 2007), and C. Sulcia muelleri (Moran et al., 2005; Figure 3).

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus