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Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic analysis of ITS clones of T. putrescentiae with the reference sequences from other related astigmatid mites (Noge et al., 2005; Liu et al., 2006; Klimov and OConnor, 2008, 2013; Yang et al., 2011; Beroiz et al., 2014). The phylogeny was inferred by Bayesian analysis of 46 partial ITS gene sequences from the observed populations, i.e., L, laboratory; K, Koppert; P, Phillips; D, Dog; H, Ham; and Z, field Zvoleneves. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.1). Branch labels indicate Bayesian posterior probabilities and supporting bootstrap values from maximum likelihood analysis.
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Figure 2: Phylogenetic analysis of ITS clones of T. putrescentiae with the reference sequences from other related astigmatid mites (Noge et al., 2005; Liu et al., 2006; Klimov and OConnor, 2008, 2013; Yang et al., 2011; Beroiz et al., 2014). The phylogeny was inferred by Bayesian analysis of 46 partial ITS gene sequences from the observed populations, i.e., L, laboratory; K, Koppert; P, Phillips; D, Dog; H, Ham; and Z, field Zvoleneves. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.1). Branch labels indicate Bayesian posterior probabilities and supporting bootstrap values from maximum likelihood analysis.

Mentions: The taxonomic comparison based on Bayesian analyses of CO1 showed that observed T. putrescentiae populations (i.e., Laboratory, Dog, Koppert, and Phillips) clustered together with the known CO1 sequences from GenBank. The exception occurred with the field populations from Zvoleneves, which formed two separate clusters: (i) four sequences (Z 1, 2, 4, 5) formed one cluster with A. siro and (ii) the next sequences clustered to Tyrophagus similis (Figure 1). Using a diagnostic CO1 dataset of 25 identified species of Tyrophagus (PBK, unpublished), we matched these four sequences with Tyrophagus fanetzhangorum. Seven other sequences clustered with T. putrescentiae, based on both GenBank data and our Tyrophagus dataset. The Bayesian analyses of ITS confirm the previous classification for the laboratory populations, Dog, Koppert, and Phillips with T. putrescentiae and similar to the CO1 data, the field Zvoleneves population (eight sequences) clustered with T. fanetzhangorum (Figure 2).


Populations of Stored Product Mite Tyrophagus putrescentiae Differ in Their Bacterial Communities.

Erban T, Klimov PB, Smrz J, Phillips TW, Nesvorna M, Kopecky J, Hubert J - Front Microbiol (2016)

Phylogenetic analysis of ITS clones of T. putrescentiae with the reference sequences from other related astigmatid mites (Noge et al., 2005; Liu et al., 2006; Klimov and OConnor, 2008, 2013; Yang et al., 2011; Beroiz et al., 2014). The phylogeny was inferred by Bayesian analysis of 46 partial ITS gene sequences from the observed populations, i.e., L, laboratory; K, Koppert; P, Phillips; D, Dog; H, Ham; and Z, field Zvoleneves. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.1). Branch labels indicate Bayesian posterior probabilities and supporting bootstrap values from maximum likelihood analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940368&req=5

Figure 2: Phylogenetic analysis of ITS clones of T. putrescentiae with the reference sequences from other related astigmatid mites (Noge et al., 2005; Liu et al., 2006; Klimov and OConnor, 2008, 2013; Yang et al., 2011; Beroiz et al., 2014). The phylogeny was inferred by Bayesian analysis of 46 partial ITS gene sequences from the observed populations, i.e., L, laboratory; K, Koppert; P, Phillips; D, Dog; H, Ham; and Z, field Zvoleneves. Branch lengths correspond to the mean posterior estimates of evolutionary distances (scale bar: 0.1). Branch labels indicate Bayesian posterior probabilities and supporting bootstrap values from maximum likelihood analysis.
Mentions: The taxonomic comparison based on Bayesian analyses of CO1 showed that observed T. putrescentiae populations (i.e., Laboratory, Dog, Koppert, and Phillips) clustered together with the known CO1 sequences from GenBank. The exception occurred with the field populations from Zvoleneves, which formed two separate clusters: (i) four sequences (Z 1, 2, 4, 5) formed one cluster with A. siro and (ii) the next sequences clustered to Tyrophagus similis (Figure 1). Using a diagnostic CO1 dataset of 25 identified species of Tyrophagus (PBK, unpublished), we matched these four sequences with Tyrophagus fanetzhangorum. Seven other sequences clustered with T. putrescentiae, based on both GenBank data and our Tyrophagus dataset. The Bayesian analyses of ITS confirm the previous classification for the laboratory populations, Dog, Koppert, and Phillips with T. putrescentiae and similar to the CO1 data, the field Zvoleneves population (eight sequences) clustered with T. fanetzhangorum (Figure 2).

Bottom Line: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations).Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

View Article: PubMed Central - PubMed

Affiliation: Biologically Active Substances in Crop Protection, Crop Research Institute Prague, Czech Republic.

ABSTRACT

Background: Tyrophagus putrescentiae colonizes different human-related habitats and feeds on various post-harvest foods. The microbiota acquired by these mites can influence the nutritional plasticity in different populations. We compared the bacterial communities of five populations of T. putrescentiae and one mixed population of T. putrescentiae and T. fanetzhangorum collected from different habitats.

Material: The bacterial communities of the six mite populations from different habitats and diets were compared by Sanger sequencing of cloned 16S rRNA obtained from amplification with universal eubacterial primers and using bacterial taxon-specific primers on the samples of adults/juveniles or eggs. Microscopic techniques were used to localize bacteria in food boli and mite bodies. The morphological determination of the mite populations was confirmed by analyses of CO1 and ITS fragment genes.

Results: The following symbiotic bacteria were found in compared mite populations: Wolbachia (two populations), Cardinium (five populations), Bartonella-like (five populations), Blattabacterium-like symbiont (three populations), and Solitalea-like (six populations). From 35 identified OTUs97, only Solitalea was identified in all populations. The next most frequent and abundant sequences were Bacillus, Moraxella, Staphylococcus, Kocuria, and Microbacterium. We suggest that some bacterial species may occasionally be ingested with food. The bacteriocytes were observed in some individuals in all mite populations. Bacteria were not visualized in food boli by staining, but bacteria were found by histological means in ovaria of Wolbachia-infested populations.

Conclusion: The presence of Blattabacterium-like, Cardinium, Wolbachia, and Solitalea-like in the eggs of T. putrescentiae indicates mother to offspring (vertical) transmission. RESULTS of this study indicate that diet and habitats influence not only the ingested bacteria but also the symbiotic bacteria of T. putrescentiae.

No MeSH data available.


Related in: MedlinePlus