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Enhanced xylose fermentation and hydrolysate inhibitor tolerance of Scheffersomyces shehatae for efficient ethanol production from non-detoxified lignocellulosic hydrolysate.

Senatham S, Chamduang T, Kaewchingduang Y, Thammasittirong A, Srisodsuk M, Elliston A, Roberts IN, Waldron KW, Thammasittirong SN - Springerplus (2016)

Bottom Line: A maximum ethanol concentration of 29.04 g/L was produced from 71.31 g/L xylose, which was 58.95 % higher than that of the wild-type.This mutant also displayed significantly improved hydrolysate inhibitors tolerance and increased ethanol production from non-detoxified lignocellulosic hydrolysates.The ethanol yield, productivity and theoretical yield by TTC79 from sugarcane bagasse hydrolysate were 0.46 g/g, 0.20 g/L/h and 90.61 %, respectively, while the corresponding values for the wild-type were 0.20 g/g, 0.04 g/L/h and 39.20 %, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand.

ABSTRACT
Effective conversion of xylose into ethanol is important for lignocellulosic ethanol production. In the present study, UV-C mutagenesis was used to improve the efficiency of xylose fermentation. The mutated Scheffersomyces shehatae strain TTC79 fermented glucose as efficiently and xylose more efficiently, producing a higher ethanol concentration than the wild-type. A maximum ethanol concentration of 29.04 g/L was produced from 71.31 g/L xylose, which was 58.95 % higher than that of the wild-type. This mutant also displayed significantly improved hydrolysate inhibitors tolerance and increased ethanol production from non-detoxified lignocellulosic hydrolysates. The ethanol yield, productivity and theoretical yield by TTC79 from sugarcane bagasse hydrolysate were 0.46 g/g, 0.20 g/L/h and 90.61 %, respectively, while the corresponding values for the wild-type were 0.20 g/g, 0.04 g/L/h and 39.20 %, respectively. These results demonstrate that S. shehatae TTC79 is a useful non-recombinant strain, combining efficient xylose consumption and high inhibitor tolerance, with potential for application in ethanol production from lignocellulose hydrolysates.

No MeSH data available.


Related in: MedlinePlus

Sugar consumption and ethanol production by TTC79 and the wild-type in synthetic medium containing glucose (a) xylose (b) and glucose/xylose mixture (c). Wild-type/ethanol (filled triangle), wild-type/glucose (filled square), wild-type/xylose (filled circle), TTC79/ethanol (open triangle), TTC79/glucose (open square), TTC79/xylose (open circle). Data represent the mean ± standard deviation from three independent experiments
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Fig1: Sugar consumption and ethanol production by TTC79 and the wild-type in synthetic medium containing glucose (a) xylose (b) and glucose/xylose mixture (c). Wild-type/ethanol (filled triangle), wild-type/glucose (filled square), wild-type/xylose (filled circle), TTC79/ethanol (open triangle), TTC79/glucose (open square), TTC79/xylose (open circle). Data represent the mean ± standard deviation from three independent experiments

Mentions: The ability of TTC79 to ferment glucose, xylose and mixed sugars in synthetic medium was investigated independently by shake-flask studies. The glucose consumption and fermentation patterns for TTC79 were similar to the wild-type (Fig. 1a). Glucose was completely consumed by both TTC79 and the wild-type within 36 h. The maximum range of ethanol concentrations produced by TTC79 and the wild-type was 41.94–42.40 g/L. These results indicated that the glucose fermentation ability was not severely affected by mutations in TTC79.Fig. 1


Enhanced xylose fermentation and hydrolysate inhibitor tolerance of Scheffersomyces shehatae for efficient ethanol production from non-detoxified lignocellulosic hydrolysate.

Senatham S, Chamduang T, Kaewchingduang Y, Thammasittirong A, Srisodsuk M, Elliston A, Roberts IN, Waldron KW, Thammasittirong SN - Springerplus (2016)

Sugar consumption and ethanol production by TTC79 and the wild-type in synthetic medium containing glucose (a) xylose (b) and glucose/xylose mixture (c). Wild-type/ethanol (filled triangle), wild-type/glucose (filled square), wild-type/xylose (filled circle), TTC79/ethanol (open triangle), TTC79/glucose (open square), TTC79/xylose (open circle). Data represent the mean ± standard deviation from three independent experiments
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940357&req=5

Fig1: Sugar consumption and ethanol production by TTC79 and the wild-type in synthetic medium containing glucose (a) xylose (b) and glucose/xylose mixture (c). Wild-type/ethanol (filled triangle), wild-type/glucose (filled square), wild-type/xylose (filled circle), TTC79/ethanol (open triangle), TTC79/glucose (open square), TTC79/xylose (open circle). Data represent the mean ± standard deviation from three independent experiments
Mentions: The ability of TTC79 to ferment glucose, xylose and mixed sugars in synthetic medium was investigated independently by shake-flask studies. The glucose consumption and fermentation patterns for TTC79 were similar to the wild-type (Fig. 1a). Glucose was completely consumed by both TTC79 and the wild-type within 36 h. The maximum range of ethanol concentrations produced by TTC79 and the wild-type was 41.94–42.40 g/L. These results indicated that the glucose fermentation ability was not severely affected by mutations in TTC79.Fig. 1

Bottom Line: A maximum ethanol concentration of 29.04 g/L was produced from 71.31 g/L xylose, which was 58.95 % higher than that of the wild-type.This mutant also displayed significantly improved hydrolysate inhibitors tolerance and increased ethanol production from non-detoxified lignocellulosic hydrolysates.The ethanol yield, productivity and theoretical yield by TTC79 from sugarcane bagasse hydrolysate were 0.46 g/g, 0.20 g/L/h and 90.61 %, respectively, while the corresponding values for the wild-type were 0.20 g/g, 0.04 g/L/h and 39.20 %, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140 Thailand.

ABSTRACT
Effective conversion of xylose into ethanol is important for lignocellulosic ethanol production. In the present study, UV-C mutagenesis was used to improve the efficiency of xylose fermentation. The mutated Scheffersomyces shehatae strain TTC79 fermented glucose as efficiently and xylose more efficiently, producing a higher ethanol concentration than the wild-type. A maximum ethanol concentration of 29.04 g/L was produced from 71.31 g/L xylose, which was 58.95 % higher than that of the wild-type. This mutant also displayed significantly improved hydrolysate inhibitors tolerance and increased ethanol production from non-detoxified lignocellulosic hydrolysates. The ethanol yield, productivity and theoretical yield by TTC79 from sugarcane bagasse hydrolysate were 0.46 g/g, 0.20 g/L/h and 90.61 %, respectively, while the corresponding values for the wild-type were 0.20 g/g, 0.04 g/L/h and 39.20 %, respectively. These results demonstrate that S. shehatae TTC79 is a useful non-recombinant strain, combining efficient xylose consumption and high inhibitor tolerance, with potential for application in ethanol production from lignocellulose hydrolysates.

No MeSH data available.


Related in: MedlinePlus