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Quantification of protein Z expression in lung adenocarcinoma tissues and cells.

Wang H, Huang F, Pan XY, Guan ZB, Zeng WB, Li MJ, Zhang RH - Springerplus (2016)

Bottom Line: In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR.Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function.Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080 Guangdong People's Republic of China.

ABSTRACT
As a regulator of coagulation, abnormal Protein Z (PZ) expression may lead to the formation of blood clots in humans. While previous studies have shown that PZ protein is altered in several types of cancer, however, additional observations are needed to understand the complex biology involved. Herein, we investigated local alterations in PZ expression in lung adenocarcinomas by measuring gene and protein expression in both cancerous and normal lung tissues. Twenty-two (22) specimens of lung adenocarcinoma and 22 specimens of normal lung tissues from human patients were compared for the expression of PZ. In addition, A549 adenocarcinoma cells were compared to a normal epithelial cell line, 16-HBE, for in vitro PZ expression. In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR. Lung adenocarcinoma tissues showed elevated expression of both PZ mRNA and protein compared with healthy tissue. Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function. Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

No MeSH data available.


Related in: MedlinePlus

Representative images from immunohistochemical staining. In lung adenocarcinoma tissue (a), an increase in PZ staining (brown) is observed. The black arrow indicates the cytoplasmic expression of PZ, while the red arrow and yellow arrow indicates the presence of PZ protein in macrophages and endothelial cells, respectively. Scale bar 50 μm. b PZ protein was not expressed in the cytoplasm of cells in healthy lung tissue. The positively stained cells are macrophages (red arrow) and endothelial cells (yellow arrow). Scale bar 50 μm
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Fig3: Representative images from immunohistochemical staining. In lung adenocarcinoma tissue (a), an increase in PZ staining (brown) is observed. The black arrow indicates the cytoplasmic expression of PZ, while the red arrow and yellow arrow indicates the presence of PZ protein in macrophages and endothelial cells, respectively. Scale bar 50 μm. b PZ protein was not expressed in the cytoplasm of cells in healthy lung tissue. The positively stained cells are macrophages (red arrow) and endothelial cells (yellow arrow). Scale bar 50 μm

Mentions: The expression of PZ in cancerous lung tissue was increased, as indicated by intense brown staining in Fig. 3a. In contrast, healthy lung tissues showed minimal expression of PZ (Fig. 3c). Overall, PZ protein appears to be expressed in macrophages and endothelial cells in the newly formed vessels, which is in line with previous findings (Sierko et al. 2012b).Fig. 3


Quantification of protein Z expression in lung adenocarcinoma tissues and cells.

Wang H, Huang F, Pan XY, Guan ZB, Zeng WB, Li MJ, Zhang RH - Springerplus (2016)

Representative images from immunohistochemical staining. In lung adenocarcinoma tissue (a), an increase in PZ staining (brown) is observed. The black arrow indicates the cytoplasmic expression of PZ, while the red arrow and yellow arrow indicates the presence of PZ protein in macrophages and endothelial cells, respectively. Scale bar 50 μm. b PZ protein was not expressed in the cytoplasm of cells in healthy lung tissue. The positively stained cells are macrophages (red arrow) and endothelial cells (yellow arrow). Scale bar 50 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940354&req=5

Fig3: Representative images from immunohistochemical staining. In lung adenocarcinoma tissue (a), an increase in PZ staining (brown) is observed. The black arrow indicates the cytoplasmic expression of PZ, while the red arrow and yellow arrow indicates the presence of PZ protein in macrophages and endothelial cells, respectively. Scale bar 50 μm. b PZ protein was not expressed in the cytoplasm of cells in healthy lung tissue. The positively stained cells are macrophages (red arrow) and endothelial cells (yellow arrow). Scale bar 50 μm
Mentions: The expression of PZ in cancerous lung tissue was increased, as indicated by intense brown staining in Fig. 3a. In contrast, healthy lung tissues showed minimal expression of PZ (Fig. 3c). Overall, PZ protein appears to be expressed in macrophages and endothelial cells in the newly formed vessels, which is in line with previous findings (Sierko et al. 2012b).Fig. 3

Bottom Line: In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR.Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function.Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080 Guangdong People's Republic of China.

ABSTRACT
As a regulator of coagulation, abnormal Protein Z (PZ) expression may lead to the formation of blood clots in humans. While previous studies have shown that PZ protein is altered in several types of cancer, however, additional observations are needed to understand the complex biology involved. Herein, we investigated local alterations in PZ expression in lung adenocarcinomas by measuring gene and protein expression in both cancerous and normal lung tissues. Twenty-two (22) specimens of lung adenocarcinoma and 22 specimens of normal lung tissues from human patients were compared for the expression of PZ. In addition, A549 adenocarcinoma cells were compared to a normal epithelial cell line, 16-HBE, for in vitro PZ expression. In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR. Lung adenocarcinoma tissues showed elevated expression of both PZ mRNA and protein compared with healthy tissue. Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function. Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

No MeSH data available.


Related in: MedlinePlus