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Quantification of protein Z expression in lung adenocarcinoma tissues and cells.

Wang H, Huang F, Pan XY, Guan ZB, Zeng WB, Li MJ, Zhang RH - Springerplus (2016)

Bottom Line: In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR.Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function.Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080 Guangdong People's Republic of China.

ABSTRACT
As a regulator of coagulation, abnormal Protein Z (PZ) expression may lead to the formation of blood clots in humans. While previous studies have shown that PZ protein is altered in several types of cancer, however, additional observations are needed to understand the complex biology involved. Herein, we investigated local alterations in PZ expression in lung adenocarcinomas by measuring gene and protein expression in both cancerous and normal lung tissues. Twenty-two (22) specimens of lung adenocarcinoma and 22 specimens of normal lung tissues from human patients were compared for the expression of PZ. In addition, A549 adenocarcinoma cells were compared to a normal epithelial cell line, 16-HBE, for in vitro PZ expression. In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR. Lung adenocarcinoma tissues showed elevated expression of both PZ mRNA and protein compared with healthy tissue. Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function. Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

No MeSH data available.


Related in: MedlinePlus

PZ mRNA in normal vs. cancerous tissue biopsies. Compared to normal tissue, PZ mRNA expression was 1.77-fold higher in lung adenocarcinoma tissue, which was statistically significant (P = 0.023). The altered PZ expression in adenocarcinoma patient samples, compared with normal tissues, suggests its involvement in the pathology of lung adenocarcinoma
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Fig2: PZ mRNA in normal vs. cancerous tissue biopsies. Compared to normal tissue, PZ mRNA expression was 1.77-fold higher in lung adenocarcinoma tissue, which was statistically significant (P = 0.023). The altered PZ expression in adenocarcinoma patient samples, compared with normal tissues, suggests its involvement in the pathology of lung adenocarcinoma

Mentions: Similarly, we used SYBR-based quantitative fluorescence PCR to detect the genetic expression of PZ in 22 lung adenocarcinoma and 22 normal tissues. A single peak was again observed, indicating that no off-target products such as primer dimers were formed. Compared with normal tissue, PZ mRNA expression was 1.77-fold higher in lung adenocarcinoma samples, which was statistically significant (Fig. 2).Fig. 2


Quantification of protein Z expression in lung adenocarcinoma tissues and cells.

Wang H, Huang F, Pan XY, Guan ZB, Zeng WB, Li MJ, Zhang RH - Springerplus (2016)

PZ mRNA in normal vs. cancerous tissue biopsies. Compared to normal tissue, PZ mRNA expression was 1.77-fold higher in lung adenocarcinoma tissue, which was statistically significant (P = 0.023). The altered PZ expression in adenocarcinoma patient samples, compared with normal tissues, suggests its involvement in the pathology of lung adenocarcinoma
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940354&req=5

Fig2: PZ mRNA in normal vs. cancerous tissue biopsies. Compared to normal tissue, PZ mRNA expression was 1.77-fold higher in lung adenocarcinoma tissue, which was statistically significant (P = 0.023). The altered PZ expression in adenocarcinoma patient samples, compared with normal tissues, suggests its involvement in the pathology of lung adenocarcinoma
Mentions: Similarly, we used SYBR-based quantitative fluorescence PCR to detect the genetic expression of PZ in 22 lung adenocarcinoma and 22 normal tissues. A single peak was again observed, indicating that no off-target products such as primer dimers were formed. Compared with normal tissue, PZ mRNA expression was 1.77-fold higher in lung adenocarcinoma samples, which was statistically significant (Fig. 2).Fig. 2

Bottom Line: In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR.Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function.Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080 Guangdong People's Republic of China.

ABSTRACT
As a regulator of coagulation, abnormal Protein Z (PZ) expression may lead to the formation of blood clots in humans. While previous studies have shown that PZ protein is altered in several types of cancer, however, additional observations are needed to understand the complex biology involved. Herein, we investigated local alterations in PZ expression in lung adenocarcinomas by measuring gene and protein expression in both cancerous and normal lung tissues. Twenty-two (22) specimens of lung adenocarcinoma and 22 specimens of normal lung tissues from human patients were compared for the expression of PZ. In addition, A549 adenocarcinoma cells were compared to a normal epithelial cell line, 16-HBE, for in vitro PZ expression. In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR. Lung adenocarcinoma tissues showed elevated expression of both PZ mRNA and protein compared with healthy tissue. Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function. Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

No MeSH data available.


Related in: MedlinePlus