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Quantification of protein Z expression in lung adenocarcinoma tissues and cells.

Wang H, Huang F, Pan XY, Guan ZB, Zeng WB, Li MJ, Zhang RH - Springerplus (2016)

Bottom Line: In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR.Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function.Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080 Guangdong People's Republic of China.

ABSTRACT
As a regulator of coagulation, abnormal Protein Z (PZ) expression may lead to the formation of blood clots in humans. While previous studies have shown that PZ protein is altered in several types of cancer, however, additional observations are needed to understand the complex biology involved. Herein, we investigated local alterations in PZ expression in lung adenocarcinomas by measuring gene and protein expression in both cancerous and normal lung tissues. Twenty-two (22) specimens of lung adenocarcinoma and 22 specimens of normal lung tissues from human patients were compared for the expression of PZ. In addition, A549 adenocarcinoma cells were compared to a normal epithelial cell line, 16-HBE, for in vitro PZ expression. In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR. Lung adenocarcinoma tissues showed elevated expression of both PZ mRNA and protein compared with healthy tissue. Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function. Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

No MeSH data available.


Related in: MedlinePlus

PZ mRNA in cell cultures. In comparison with normal cells, A549 adenocarcinoma cells exhibited a 1.06-fold increase in PZ mRNA expression. (P = 0.086)
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Fig1: PZ mRNA in cell cultures. In comparison with normal cells, A549 adenocarcinoma cells exhibited a 1.06-fold increase in PZ mRNA expression. (P = 0.086)

Mentions: We used SYBR-based quantitative fluorescence PCR to detect the expression of PZ gene in the A549 lung adenocarcinoma cell line and 16-HBE normal epithelial line, using GADPH to normalize the samples. The dissociation curves for both genes showed a single peak, suggesting that no off-target products, such as primer dimers, were formed. A549 adenocarcinoma cells exhibited a 1.06-fold increase in PZ mRNA expression over 16-HBE cells, but this increase was not statistically significant (Fig. 1).Fig. 1


Quantification of protein Z expression in lung adenocarcinoma tissues and cells.

Wang H, Huang F, Pan XY, Guan ZB, Zeng WB, Li MJ, Zhang RH - Springerplus (2016)

PZ mRNA in cell cultures. In comparison with normal cells, A549 adenocarcinoma cells exhibited a 1.06-fold increase in PZ mRNA expression. (P = 0.086)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940354&req=5

Fig1: PZ mRNA in cell cultures. In comparison with normal cells, A549 adenocarcinoma cells exhibited a 1.06-fold increase in PZ mRNA expression. (P = 0.086)
Mentions: We used SYBR-based quantitative fluorescence PCR to detect the expression of PZ gene in the A549 lung adenocarcinoma cell line and 16-HBE normal epithelial line, using GADPH to normalize the samples. The dissociation curves for both genes showed a single peak, suggesting that no off-target products, such as primer dimers, were formed. A549 adenocarcinoma cells exhibited a 1.06-fold increase in PZ mRNA expression over 16-HBE cells, but this increase was not statistically significant (Fig. 1).Fig. 1

Bottom Line: In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR.Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function.Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Hematology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, 510080 Guangdong People's Republic of China.

ABSTRACT
As a regulator of coagulation, abnormal Protein Z (PZ) expression may lead to the formation of blood clots in humans. While previous studies have shown that PZ protein is altered in several types of cancer, however, additional observations are needed to understand the complex biology involved. Herein, we investigated local alterations in PZ expression in lung adenocarcinomas by measuring gene and protein expression in both cancerous and normal lung tissues. Twenty-two (22) specimens of lung adenocarcinoma and 22 specimens of normal lung tissues from human patients were compared for the expression of PZ. In addition, A549 adenocarcinoma cells were compared to a normal epithelial cell line, 16-HBE, for in vitro PZ expression. In tissues and cells, PZ protein and gene expression were determined using western blot, immunohistochemistry and PCR. Lung adenocarcinoma tissues showed elevated expression of both PZ mRNA and protein compared with healthy tissue. Only protein expression was increased in cultured cell lines, which holds implications for the dominant source of PZ in tissues, as well as protein modifications necessary for PZ function. Protein Z appears to be associated with the presence of lung adenocarcinoma and may be a viable prognostic biomarker for lung cancer.

No MeSH data available.


Related in: MedlinePlus