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Delineation of attenuation of oxidative stress and mutagenic stress by Murraya exotica L. leaves.

Kaur D, Kaur A, Arora S - Springerplus (2016)

Bottom Line: It was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g.It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays.Overall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen.

View Article: PubMed Central - PubMed

Affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.

ABSTRACT

Background: Murraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and also known for its ethnobotanical importance. With this in mind, the plant was chosen and leaves were extracted sequentially to obtain ethanol, hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The extract/fractions were evaluated for total phenolic and flavonoid content by spectrophotometric methods. The UHPLC technique was employed for profiling the different polyphenolic compounds present in the extract/fractions. Furthermore, the extract/fractions were analysed for the antioxidant and antimutagenic activities using different in vitro assays.

Results: It was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g. Likewise, UHPLC method also showed maximum number as well as amount of polyphenolic compounds in ethyl acetate fraction. It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays. In Ames assay, ethyl acetate fraction again showed maximum antimutagenic activity of 97.70 % against the 2-aminofluorene mutagen in TA100 tester strain of Salmonella typhimurium.

Conclusion: Overall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen. The maximum bioactivities of the ethyl acetate fraction may be linked to the presence of high number of polyphenolic compounds as shown by spectrophotometric as well as UHPLC methods.

No MeSH data available.


Related in: MedlinePlus

DNA nicking assay for non site-specific ˙OH scavenging activity of ethanol extract (a), hexane (b), chloroform (c), ethyl acetate (d), n-butanol (e), aqueous fractions (f) of M. exotica leaves. Lane 1 represents: pBR322 DNA + distilled water; lane 2 pBR322 DNA + distilled water + Fenton Reagent (FR); lane 3 pBR322 DNA + FR + Rutin; lane 4–8 pBR322 DNA + FR + Extract/fraction (1000, 800, 600, 400 and 200 µg/ml respectively)
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Fig3: DNA nicking assay for non site-specific ˙OH scavenging activity of ethanol extract (a), hexane (b), chloroform (c), ethyl acetate (d), n-butanol (e), aqueous fractions (f) of M. exotica leaves. Lane 1 represents: pBR322 DNA + distilled water; lane 2 pBR322 DNA + distilled water + Fenton Reagent (FR); lane 3 pBR322 DNA + FR + Rutin; lane 4–8 pBR322 DNA + FR + Extract/fraction (1000, 800, 600, 400 and 200 µg/ml respectively)

Mentions: The hydroxyl radical scavenging ability of different concentrations of extract/fractions was observed by DNA nicking assay. As shown in Table 3 and Fig. 3, the presence of extract/fractions retained the maximum amount of supercoiled DNA in its native form (form I) by scavenging the hydroxyl radicals as generated by Fenton’s reagent. From the densitometric analysis, it was found that ethyl acetate fraction significantly conserved the 52.8 % of DNA in its native form by reducing the hydroxyl mediated breaking, conversion of supercoiled DNA into open circular (form II) and linear (form III) via scavenging the hydroxyl radicals in a dose dependent manner and thus providing the significant protection against oxidative stress.Table 3


Delineation of attenuation of oxidative stress and mutagenic stress by Murraya exotica L. leaves.

Kaur D, Kaur A, Arora S - Springerplus (2016)

DNA nicking assay for non site-specific ˙OH scavenging activity of ethanol extract (a), hexane (b), chloroform (c), ethyl acetate (d), n-butanol (e), aqueous fractions (f) of M. exotica leaves. Lane 1 represents: pBR322 DNA + distilled water; lane 2 pBR322 DNA + distilled water + Fenton Reagent (FR); lane 3 pBR322 DNA + FR + Rutin; lane 4–8 pBR322 DNA + FR + Extract/fraction (1000, 800, 600, 400 and 200 µg/ml respectively)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940350&req=5

Fig3: DNA nicking assay for non site-specific ˙OH scavenging activity of ethanol extract (a), hexane (b), chloroform (c), ethyl acetate (d), n-butanol (e), aqueous fractions (f) of M. exotica leaves. Lane 1 represents: pBR322 DNA + distilled water; lane 2 pBR322 DNA + distilled water + Fenton Reagent (FR); lane 3 pBR322 DNA + FR + Rutin; lane 4–8 pBR322 DNA + FR + Extract/fraction (1000, 800, 600, 400 and 200 µg/ml respectively)
Mentions: The hydroxyl radical scavenging ability of different concentrations of extract/fractions was observed by DNA nicking assay. As shown in Table 3 and Fig. 3, the presence of extract/fractions retained the maximum amount of supercoiled DNA in its native form (form I) by scavenging the hydroxyl radicals as generated by Fenton’s reagent. From the densitometric analysis, it was found that ethyl acetate fraction significantly conserved the 52.8 % of DNA in its native form by reducing the hydroxyl mediated breaking, conversion of supercoiled DNA into open circular (form II) and linear (form III) via scavenging the hydroxyl radicals in a dose dependent manner and thus providing the significant protection against oxidative stress.Table 3

Bottom Line: It was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g.It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays.Overall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen.

View Article: PubMed Central - PubMed

Affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.

ABSTRACT

Background: Murraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and also known for its ethnobotanical importance. With this in mind, the plant was chosen and leaves were extracted sequentially to obtain ethanol, hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The extract/fractions were evaluated for total phenolic and flavonoid content by spectrophotometric methods. The UHPLC technique was employed for profiling the different polyphenolic compounds present in the extract/fractions. Furthermore, the extract/fractions were analysed for the antioxidant and antimutagenic activities using different in vitro assays.

Results: It was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g. Likewise, UHPLC method also showed maximum number as well as amount of polyphenolic compounds in ethyl acetate fraction. It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays. In Ames assay, ethyl acetate fraction again showed maximum antimutagenic activity of 97.70 % against the 2-aminofluorene mutagen in TA100 tester strain of Salmonella typhimurium.

Conclusion: Overall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen. The maximum bioactivities of the ethyl acetate fraction may be linked to the presence of high number of polyphenolic compounds as shown by spectrophotometric as well as UHPLC methods.

No MeSH data available.


Related in: MedlinePlus