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Delineation of attenuation of oxidative stress and mutagenic stress by Murraya exotica L. leaves.

Kaur D, Kaur A, Arora S - Springerplus (2016)

Bottom Line: It was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g.It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays.Overall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen.

View Article: PubMed Central - PubMed

Affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.

ABSTRACT

Background: Murraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and also known for its ethnobotanical importance. With this in mind, the plant was chosen and leaves were extracted sequentially to obtain ethanol, hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The extract/fractions were evaluated for total phenolic and flavonoid content by spectrophotometric methods. The UHPLC technique was employed for profiling the different polyphenolic compounds present in the extract/fractions. Furthermore, the extract/fractions were analysed for the antioxidant and antimutagenic activities using different in vitro assays.

Results: It was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g. Likewise, UHPLC method also showed maximum number as well as amount of polyphenolic compounds in ethyl acetate fraction. It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays. In Ames assay, ethyl acetate fraction again showed maximum antimutagenic activity of 97.70 % against the 2-aminofluorene mutagen in TA100 tester strain of Salmonella typhimurium.

Conclusion: Overall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen. The maximum bioactivities of the ethyl acetate fraction may be linked to the presence of high number of polyphenolic compounds as shown by spectrophotometric as well as UHPLC methods.

No MeSH data available.


Related in: MedlinePlus

Antioxidant activity of different concentrations of extract/fractions of M. exotica leaves accessed by DPPH radical scavenging assay (a), ABTS radical cation decolorization assay (b), FRAP assay (c), Cupric-Ion-Reducing Antioxidant Capacity (CUPRAC) assay (d), Molybdate ion Reduction assay (e). Data expressed as mean of % inhibition ± SE of triplicates
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Fig2: Antioxidant activity of different concentrations of extract/fractions of M. exotica leaves accessed by DPPH radical scavenging assay (a), ABTS radical cation decolorization assay (b), FRAP assay (c), Cupric-Ion-Reducing Antioxidant Capacity (CUPRAC) assay (d), Molybdate ion Reduction assay (e). Data expressed as mean of % inhibition ± SE of triplicates

Mentions: The decolorizing activity following the trapping of unpaired electron of DPPH and ABTS radical cation was used as a measure of free radical scavenging activity (Fig. 2a, b). The radical scavenging activity of extract/fractions exhibited the dose dependent response in DPPH assay. The most effective free radical scavengers were found in ethyl acetate fraction with 90.77 % decolorization at concentration of 1000 µg/ml whereas ascorbic acid (positive control) showed 91.96 % of decolorization at 100 µg/ml concentration. Likewise, n-butanol, ethanol, aqueous, chloroform and hexane fractions exhibited the 70.05, 45.28, 28.27, 20.25 and 16.09 % of decolorization activity respectively. In ABTS radical cation assay, all extract/fractions showed dose dependent free radical scavenging activity with highest potential (92.80 %) of ethyl acetate fraction which was found to be comparable to positive control (rutin). The ABTS radical cation decolorization activity was found in the order of ethyl acetate > n-butanol > ethanol > aqueous > chloroform, and least in hexane fraction.Fig. 2


Delineation of attenuation of oxidative stress and mutagenic stress by Murraya exotica L. leaves.

Kaur D, Kaur A, Arora S - Springerplus (2016)

Antioxidant activity of different concentrations of extract/fractions of M. exotica leaves accessed by DPPH radical scavenging assay (a), ABTS radical cation decolorization assay (b), FRAP assay (c), Cupric-Ion-Reducing Antioxidant Capacity (CUPRAC) assay (d), Molybdate ion Reduction assay (e). Data expressed as mean of % inhibition ± SE of triplicates
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940350&req=5

Fig2: Antioxidant activity of different concentrations of extract/fractions of M. exotica leaves accessed by DPPH radical scavenging assay (a), ABTS radical cation decolorization assay (b), FRAP assay (c), Cupric-Ion-Reducing Antioxidant Capacity (CUPRAC) assay (d), Molybdate ion Reduction assay (e). Data expressed as mean of % inhibition ± SE of triplicates
Mentions: The decolorizing activity following the trapping of unpaired electron of DPPH and ABTS radical cation was used as a measure of free radical scavenging activity (Fig. 2a, b). The radical scavenging activity of extract/fractions exhibited the dose dependent response in DPPH assay. The most effective free radical scavengers were found in ethyl acetate fraction with 90.77 % decolorization at concentration of 1000 µg/ml whereas ascorbic acid (positive control) showed 91.96 % of decolorization at 100 µg/ml concentration. Likewise, n-butanol, ethanol, aqueous, chloroform and hexane fractions exhibited the 70.05, 45.28, 28.27, 20.25 and 16.09 % of decolorization activity respectively. In ABTS radical cation assay, all extract/fractions showed dose dependent free radical scavenging activity with highest potential (92.80 %) of ethyl acetate fraction which was found to be comparable to positive control (rutin). The ABTS radical cation decolorization activity was found in the order of ethyl acetate > n-butanol > ethanol > aqueous > chloroform, and least in hexane fraction.Fig. 2

Bottom Line: It was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g.It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays.Overall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen.

View Article: PubMed Central - PubMed

Affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.

ABSTRACT

Background: Murraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and also known for its ethnobotanical importance. With this in mind, the plant was chosen and leaves were extracted sequentially to obtain ethanol, hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The extract/fractions were evaluated for total phenolic and flavonoid content by spectrophotometric methods. The UHPLC technique was employed for profiling the different polyphenolic compounds present in the extract/fractions. Furthermore, the extract/fractions were analysed for the antioxidant and antimutagenic activities using different in vitro assays.

Results: It was observed that, among the different extract/fractions, ethyl acetate fraction exhibited maximum total phenolic content i.e. 119.6 mg of GAE/g whereas chloroform fraction showed maximum total flavonoid content i.e. 323.5 mg of RE/g. Likewise, UHPLC method also showed maximum number as well as amount of polyphenolic compounds in ethyl acetate fraction. It was further analyzed that the same fraction exhibited the maximum radical scavenging activity in all antioxidant assays. In Ames assay, ethyl acetate fraction again showed maximum antimutagenic activity of 97.70 % against the 2-aminofluorene mutagen in TA100 tester strain of Salmonella typhimurium.

Conclusion: Overall, among all the extract/fractions, ethyl acetate fraction was observed to be highly effective in scavenging the free radical as well as reducing the mutagenic effect of the mutagen. The maximum bioactivities of the ethyl acetate fraction may be linked to the presence of high number of polyphenolic compounds as shown by spectrophotometric as well as UHPLC methods.

No MeSH data available.


Related in: MedlinePlus