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Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Shephard F, Greville-Heygate O, Liddell S, Emes R, Chakrabarti L - Mitochondrion (2016)

Bottom Line: We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles.These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell.It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's.

View Article: PubMed Central - PubMed

Affiliation: University of Nottingham, Faculty of Medicine, SVMS, Sutton Bonington Campus, LE12 5RD, England, UK.

No MeSH data available.


Related in: MedlinePlus

Ratio of mitochondrial versus cytoplasmic HbB in controls and Parkinson's cerebellum with increase in PMI. Control samples show a general decrease in HbB. The lower plot shows values for male and female HbB and is also coded for PMI category illustrating the lack of relationship between PMI and HbB ratios.
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f0030: Ratio of mitochondrial versus cytoplasmic HbB in controls and Parkinson's cerebellum with increase in PMI. Control samples show a general decrease in HbB. The lower plot shows values for male and female HbB and is also coded for PMI category illustrating the lack of relationship between PMI and HbB ratios.

Mentions: In order to account for any protein changes with regard to post mortem interval (PMI) we plotted HbB levels and also the mitochondrial marker COXIV against the delay after death in hours (Supplementary Fig. 3, Supplementary Fig. 4). These confirm that the levels of HbB we see against disease duration is not an effect of tissue changes after death has occurred. Brain banks usually have fewer female Parkinson's disease brains in their collections. We have analysed the full dataset we obtained but in order to be sure that differences in the numbers of females and males analysed did not skew our dataset we disease stage and age matched each female Parkinson's brain mitochondrial fraction (n = 7) with a male brain (n = 7) mitochondrial fraction and re-ran the data to show the gender difference is upheld with equal numbers of samples from each gender (Supplementary Fig. 6). Multiple linear regression analysis (software Genstat) was performed to verify that PMI is not a confounding factor in our analyses with disease duration – Supplementary Table 3.


Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Shephard F, Greville-Heygate O, Liddell S, Emes R, Chakrabarti L - Mitochondrion (2016)

Ratio of mitochondrial versus cytoplasmic HbB in controls and Parkinson's cerebellum with increase in PMI. Control samples show a general decrease in HbB. The lower plot shows values for male and female HbB and is also coded for PMI category illustrating the lack of relationship between PMI and HbB ratios.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940210&req=5

f0030: Ratio of mitochondrial versus cytoplasmic HbB in controls and Parkinson's cerebellum with increase in PMI. Control samples show a general decrease in HbB. The lower plot shows values for male and female HbB and is also coded for PMI category illustrating the lack of relationship between PMI and HbB ratios.
Mentions: In order to account for any protein changes with regard to post mortem interval (PMI) we plotted HbB levels and also the mitochondrial marker COXIV against the delay after death in hours (Supplementary Fig. 3, Supplementary Fig. 4). These confirm that the levels of HbB we see against disease duration is not an effect of tissue changes after death has occurred. Brain banks usually have fewer female Parkinson's disease brains in their collections. We have analysed the full dataset we obtained but in order to be sure that differences in the numbers of females and males analysed did not skew our dataset we disease stage and age matched each female Parkinson's brain mitochondrial fraction (n = 7) with a male brain (n = 7) mitochondrial fraction and re-ran the data to show the gender difference is upheld with equal numbers of samples from each gender (Supplementary Fig. 6). Multiple linear regression analysis (software Genstat) was performed to verify that PMI is not a confounding factor in our analyses with disease duration – Supplementary Table 3.

Bottom Line: We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles.These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell.It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's.

View Article: PubMed Central - PubMed

Affiliation: University of Nottingham, Faculty of Medicine, SVMS, Sutton Bonington Campus, LE12 5RD, England, UK.

No MeSH data available.


Related in: MedlinePlus