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Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Shephard F, Greville-Heygate O, Liddell S, Emes R, Chakrabarti L - Mitochondrion (2016)

Bottom Line: We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles.These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell.It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's.

View Article: PubMed Central - PubMed

Affiliation: University of Nottingham, Faculty of Medicine, SVMS, Sutton Bonington Campus, LE12 5RD, England, UK.

No MeSH data available.


Related in: MedlinePlus

The cellular distribution of HbB appears changed in female cerebellum with disease duration. HbB and COXIV antibodies were utilised to visualise the physical location of mitochondria and HbB in the cerebellum of female individuals with different disease durations. It appears that with longer disease duration the overall quantity of COXIV is reduced and the location of HbB shifts in the longest disease course from in and around Purkinje cells observed in controls, to the granule cell layer in sections from 18 years disease duration. DAPI is used to stain nuclei. Filter sets used are detailed in the methodology.
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f0020: The cellular distribution of HbB appears changed in female cerebellum with disease duration. HbB and COXIV antibodies were utilised to visualise the physical location of mitochondria and HbB in the cerebellum of female individuals with different disease durations. It appears that with longer disease duration the overall quantity of COXIV is reduced and the location of HbB shifts in the longest disease course from in and around Purkinje cells observed in controls, to the granule cell layer in sections from 18 years disease duration. DAPI is used to stain nuclei. Filter sets used are detailed in the methodology.

Mentions: Using antibodies to HbB and COXIV (for mitochondria) we localised mitochondrial haemoglobin in the female cerebellum (Fig. 4). In the female control cerebellum large Purkinje cells are labelled with both antibodies demonstrating the presence of mitochondrial HbB in the cell body. The cerebellum examined from a female patient 9 years into the disease suggests an overall decrease of mitochondrial HbB and mitochondria too. The patient shown with disease duration of 18 years appears to show COXIV staining in a different pattern again through the cerebellum, moving away from the Purkinje cell layer. HbB staining is increasingly distant from the Purkinje cell layer region of the cerebellum. Purkinje cell staining is relatively reduced. The brightness of the COXIV stain appears decreased in both affected brains. All the images presented were stained and imaged in a single batch to ensure valid qualitative comparison between images. The changes appear to be related to HbB protein localisation and this is something that needs to be looked into further.


Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Shephard F, Greville-Heygate O, Liddell S, Emes R, Chakrabarti L - Mitochondrion (2016)

The cellular distribution of HbB appears changed in female cerebellum with disease duration. HbB and COXIV antibodies were utilised to visualise the physical location of mitochondria and HbB in the cerebellum of female individuals with different disease durations. It appears that with longer disease duration the overall quantity of COXIV is reduced and the location of HbB shifts in the longest disease course from in and around Purkinje cells observed in controls, to the granule cell layer in sections from 18 years disease duration. DAPI is used to stain nuclei. Filter sets used are detailed in the methodology.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940210&req=5

f0020: The cellular distribution of HbB appears changed in female cerebellum with disease duration. HbB and COXIV antibodies were utilised to visualise the physical location of mitochondria and HbB in the cerebellum of female individuals with different disease durations. It appears that with longer disease duration the overall quantity of COXIV is reduced and the location of HbB shifts in the longest disease course from in and around Purkinje cells observed in controls, to the granule cell layer in sections from 18 years disease duration. DAPI is used to stain nuclei. Filter sets used are detailed in the methodology.
Mentions: Using antibodies to HbB and COXIV (for mitochondria) we localised mitochondrial haemoglobin in the female cerebellum (Fig. 4). In the female control cerebellum large Purkinje cells are labelled with both antibodies demonstrating the presence of mitochondrial HbB in the cell body. The cerebellum examined from a female patient 9 years into the disease suggests an overall decrease of mitochondrial HbB and mitochondria too. The patient shown with disease duration of 18 years appears to show COXIV staining in a different pattern again through the cerebellum, moving away from the Purkinje cell layer. HbB staining is increasingly distant from the Purkinje cell layer region of the cerebellum. Purkinje cell staining is relatively reduced. The brightness of the COXIV stain appears decreased in both affected brains. All the images presented were stained and imaged in a single batch to ensure valid qualitative comparison between images. The changes appear to be related to HbB protein localisation and this is something that needs to be looked into further.

Bottom Line: We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles.These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell.It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's.

View Article: PubMed Central - PubMed

Affiliation: University of Nottingham, Faculty of Medicine, SVMS, Sutton Bonington Campus, LE12 5RD, England, UK.

No MeSH data available.


Related in: MedlinePlus