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Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Shephard F, Greville-Heygate O, Liddell S, Emes R, Chakrabarti L - Mitochondrion (2016)

Bottom Line: We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles.These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell.It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's.

View Article: PubMed Central - PubMed

Affiliation: University of Nottingham, Faculty of Medicine, SVMS, Sutton Bonington Campus, LE12 5RD, England, UK.

No MeSH data available.


Related in: MedlinePlus

Scatterplots of mitochondrial/cytoplasmic Hb ratio versus disease duration in years. Scatterplots of the ratio of mitochondrial/cytoplasmic heamoglobin A and B in three tissues. Patient samples are split by sex and a linear model used to fit a trendline. The shaded areas show the 95% confidence limits of the fitted line. Calculations and plotting was conducted using ggplot in R (see supplemental R script which was used for generating figures). Orange circles (female), blue circles (male). Yr – years. Ctx – Cortex, Cer – Cerebellum, SN – Substantia nigra.
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f0015: Scatterplots of mitochondrial/cytoplasmic Hb ratio versus disease duration in years. Scatterplots of the ratio of mitochondrial/cytoplasmic heamoglobin A and B in three tissues. Patient samples are split by sex and a linear model used to fit a trendline. The shaded areas show the 95% confidence limits of the fitted line. Calculations and plotting was conducted using ggplot in R (see supplemental R script which was used for generating figures). Orange circles (female), blue circles (male). Yr – years. Ctx – Cortex, Cer – Cerebellum, SN – Substantia nigra.

Mentions: Western blotting was conducted as previously described (Shephard et al., 2014). Primary antibodies used were: Hba sc-21005 (Santa Cruz) 1:1000; and ab102758 (Abcam) 1:500 – for fly hypoxia; Hbb sc-22718 (Santa Cruz) 1:1000; COXIV ab16056 (Abcam) 1:1000; beta-actin ab8227 (Abcam) 1:4000; HSP-90 ab13495 (Abcam) 1:500; VDAC/Porin ab15895 (Abcam) 1:2000; NDUFS3 ab110246 (Abcam) 1:1000; SMAC/Diablo ab8115 (Abcam) 1:1000; dilution in 3% (w/v) BSA in TBS-T. Band densities were measured using Image J and samples were normalised to beta-actin. Using the normalised values the ratio of mitochondrial/cytoplasmic HbA and HbB were calculated. Data were analysed using the R statistical package http://www.r-project.org/ see supplemental data for script used to generate Fig. 3.


Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Shephard F, Greville-Heygate O, Liddell S, Emes R, Chakrabarti L - Mitochondrion (2016)

Scatterplots of mitochondrial/cytoplasmic Hb ratio versus disease duration in years. Scatterplots of the ratio of mitochondrial/cytoplasmic heamoglobin A and B in three tissues. Patient samples are split by sex and a linear model used to fit a trendline. The shaded areas show the 95% confidence limits of the fitted line. Calculations and plotting was conducted using ggplot in R (see supplemental R script which was used for generating figures). Orange circles (female), blue circles (male). Yr – years. Ctx – Cortex, Cer – Cerebellum, SN – Substantia nigra.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940210&req=5

f0015: Scatterplots of mitochondrial/cytoplasmic Hb ratio versus disease duration in years. Scatterplots of the ratio of mitochondrial/cytoplasmic heamoglobin A and B in three tissues. Patient samples are split by sex and a linear model used to fit a trendline. The shaded areas show the 95% confidence limits of the fitted line. Calculations and plotting was conducted using ggplot in R (see supplemental R script which was used for generating figures). Orange circles (female), blue circles (male). Yr – years. Ctx – Cortex, Cer – Cerebellum, SN – Substantia nigra.
Mentions: Western blotting was conducted as previously described (Shephard et al., 2014). Primary antibodies used were: Hba sc-21005 (Santa Cruz) 1:1000; and ab102758 (Abcam) 1:500 – for fly hypoxia; Hbb sc-22718 (Santa Cruz) 1:1000; COXIV ab16056 (Abcam) 1:1000; beta-actin ab8227 (Abcam) 1:4000; HSP-90 ab13495 (Abcam) 1:500; VDAC/Porin ab15895 (Abcam) 1:2000; NDUFS3 ab110246 (Abcam) 1:1000; SMAC/Diablo ab8115 (Abcam) 1:1000; dilution in 3% (w/v) BSA in TBS-T. Band densities were measured using Image J and samples were normalised to beta-actin. Using the normalised values the ratio of mitochondrial/cytoplasmic HbA and HbB were calculated. Data were analysed using the R statistical package http://www.r-project.org/ see supplemental data for script used to generate Fig. 3.

Bottom Line: We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles.These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell.It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's.

View Article: PubMed Central - PubMed

Affiliation: University of Nottingham, Faculty of Medicine, SVMS, Sutton Bonington Campus, LE12 5RD, England, UK.

No MeSH data available.


Related in: MedlinePlus