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Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Shephard F, Greville-Heygate O, Liddell S, Emes R, Chakrabarti L - Mitochondrion (2016)

Bottom Line: We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles.These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell.It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's.

View Article: PubMed Central - PubMed

Affiliation: University of Nottingham, Faculty of Medicine, SVMS, Sutton Bonington Campus, LE12 5RD, England, UK.

No MeSH data available.


Related in: MedlinePlus

Representative gel images for mitochondrial/cytoplasmic Hb ratios - normalised to beta actin, were determined by Western blotting. COXIV antibody was utilised for quality control of mitochondrial versus cytoplasmic fractions.To summarise data, samples were grouped into age ranges (1 $_amp_$lt; 70, 2 70–79, 3 ≥ 80) and separated by sex and diagnosis type (control, late PD: late stage disease diagnosed over the age of 60, early PD: early stage disease diagnosed over the age of 60, young PD: early onset disease diagnosed under the age of 60). Values were visualised as boxplots. Boxplots show the median (line), interquartile range (box) and whiskers extend to 1.5 × the Inter quartile range. Extreme values beyond the whiskers are shown as circles. Ctx – Cortex, Cer – Cerebellum, SN – Substantia nigra. C M - cytoplasmic and mitochondrial fractions extracted from the same sample. Mito/cyto – ratio of mitochondrial Hb compared with cytoplasmic Hb. F-female, M – male. Full densitometry dataset is provided in Supplementary Table 2.
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f0010: Representative gel images for mitochondrial/cytoplasmic Hb ratios - normalised to beta actin, were determined by Western blotting. COXIV antibody was utilised for quality control of mitochondrial versus cytoplasmic fractions.To summarise data, samples were grouped into age ranges (1 $_amp_$lt; 70, 2 70–79, 3 ≥ 80) and separated by sex and diagnosis type (control, late PD: late stage disease diagnosed over the age of 60, early PD: early stage disease diagnosed over the age of 60, young PD: early onset disease diagnosed under the age of 60). Values were visualised as boxplots. Boxplots show the median (line), interquartile range (box) and whiskers extend to 1.5 × the Inter quartile range. Extreme values beyond the whiskers are shown as circles. Ctx – Cortex, Cer – Cerebellum, SN – Substantia nigra. C M - cytoplasmic and mitochondrial fractions extracted from the same sample. Mito/cyto – ratio of mitochondrial Hb compared with cytoplasmic Hb. F-female, M – male. Full densitometry dataset is provided in Supplementary Table 2.

Mentions: Data for calculating alpha and beta globin ratios were collected by western blot. Fractionated cell extracts were produced and run to give cytoplasmic versus mitochondrial ratios (Fig. 2). Ratios were calculated with reference to beta actin levels in the same lane. COXIV antibody indicated enrichment of mitochondrial fractions. Cortex, cerebellum and substantia nigra regions of the brain were examined for each sample. Using the arbitrary grouping of early Parkinson's (within 10 years of onset), late Parkinson's (beyond 10 years of disease) and young Parkinson's (onset before 60 years) we were unable to detect any major differences in mitochondrial/cytoplasmic ratios.


Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Shephard F, Greville-Heygate O, Liddell S, Emes R, Chakrabarti L - Mitochondrion (2016)

Representative gel images for mitochondrial/cytoplasmic Hb ratios - normalised to beta actin, were determined by Western blotting. COXIV antibody was utilised for quality control of mitochondrial versus cytoplasmic fractions.To summarise data, samples were grouped into age ranges (1 $_amp_$lt; 70, 2 70–79, 3 ≥ 80) and separated by sex and diagnosis type (control, late PD: late stage disease diagnosed over the age of 60, early PD: early stage disease diagnosed over the age of 60, young PD: early onset disease diagnosed under the age of 60). Values were visualised as boxplots. Boxplots show the median (line), interquartile range (box) and whiskers extend to 1.5 × the Inter quartile range. Extreme values beyond the whiskers are shown as circles. Ctx – Cortex, Cer – Cerebellum, SN – Substantia nigra. C M - cytoplasmic and mitochondrial fractions extracted from the same sample. Mito/cyto – ratio of mitochondrial Hb compared with cytoplasmic Hb. F-female, M – male. Full densitometry dataset is provided in Supplementary Table 2.
© Copyright Policy - CC BY
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4940210&req=5

f0010: Representative gel images for mitochondrial/cytoplasmic Hb ratios - normalised to beta actin, were determined by Western blotting. COXIV antibody was utilised for quality control of mitochondrial versus cytoplasmic fractions.To summarise data, samples were grouped into age ranges (1 $_amp_$lt; 70, 2 70–79, 3 ≥ 80) and separated by sex and diagnosis type (control, late PD: late stage disease diagnosed over the age of 60, early PD: early stage disease diagnosed over the age of 60, young PD: early onset disease diagnosed under the age of 60). Values were visualised as boxplots. Boxplots show the median (line), interquartile range (box) and whiskers extend to 1.5 × the Inter quartile range. Extreme values beyond the whiskers are shown as circles. Ctx – Cortex, Cer – Cerebellum, SN – Substantia nigra. C M - cytoplasmic and mitochondrial fractions extracted from the same sample. Mito/cyto – ratio of mitochondrial Hb compared with cytoplasmic Hb. F-female, M – male. Full densitometry dataset is provided in Supplementary Table 2.
Mentions: Data for calculating alpha and beta globin ratios were collected by western blot. Fractionated cell extracts were produced and run to give cytoplasmic versus mitochondrial ratios (Fig. 2). Ratios were calculated with reference to beta actin levels in the same lane. COXIV antibody indicated enrichment of mitochondrial fractions. Cortex, cerebellum and substantia nigra regions of the brain were examined for each sample. Using the arbitrary grouping of early Parkinson's (within 10 years of onset), late Parkinson's (beyond 10 years of disease) and young Parkinson's (onset before 60 years) we were unable to detect any major differences in mitochondrial/cytoplasmic ratios.

Bottom Line: We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles.These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell.It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's.

View Article: PubMed Central - PubMed

Affiliation: University of Nottingham, Faculty of Medicine, SVMS, Sutton Bonington Campus, LE12 5RD, England, UK.

No MeSH data available.


Related in: MedlinePlus