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A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs.

Borggren M, Nielsen J, Karlsson I, Dalgaard TS, Trebbien R, Williams JA, Fomsgaard A - Vaccine (2016)

Bottom Line: Both the antibody-mediated and the recall lymphocyte immune responses demonstrated high reactivity against vaccine-specific strains and cross-reactivity to vaccine-heterologous strains.The results suggest that polyvalent DNA influenza vaccination may provide a strong tool for broad protection against swine influenza strains threatening animal as well as public health.In addition, the needle-free administration technique used for this DNA vaccine will provide an easy and practical approach for the large-scale vaccination of swine.

View Article: PubMed Central - PubMed

Affiliation: Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Electronic address: mabo@ssi.dk.

No MeSH data available.


Related in: MedlinePlus

T cell sub-population proliferation response to in vitro re-stimulation with influenza proteins. Pigs were vaccinated twice (day 0 and 21pv1) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). PBMC from the vaccinated pigs on day 35pv1 were cultured in vitro in the presence of recombinant influenza NP 2009, NP 1918, M1 1918 and HA 2009. After 6 days, the cells were stained with anti-CD3, -CD4, and -CD8 monoclonal antibodies and analyzed by flow cytometry. Three T cell subsets were identified based on their CD4 and CD8 expression: (a) CD4-CD8+, (b) CD4+CD8+ and (c) CD4+CD8- cells. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: **: p < 0.01; *: p < 0.05.
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fig0025: T cell sub-population proliferation response to in vitro re-stimulation with influenza proteins. Pigs were vaccinated twice (day 0 and 21pv1) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). PBMC from the vaccinated pigs on day 35pv1 were cultured in vitro in the presence of recombinant influenza NP 2009, NP 1918, M1 1918 and HA 2009. After 6 days, the cells were stained with anti-CD3, -CD4, and -CD8 monoclonal antibodies and analyzed by flow cytometry. Three T cell subsets were identified based on their CD4 and CD8 expression: (a) CD4-CD8+, (b) CD4+CD8+ and (c) CD4+CD8- cells. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: **: p < 0.01; *: p < 0.05.

Mentions: The DNA vaccine elicited NP-, M1- and HA-specific CD4-CD8+ T cells and CD4+CD8+T cells producing IFN-γ (Fig. 4a and b). The IFN-γ response levels correlated with the DNA vaccine doses. Both vaccine-homologous and vaccine-heterologous NP (1918 and 2009, respectively) could re-stimulate the PBMC, and their respective IFN-γ responses correlated significantly (r = 0.78, p < 0.0001 (Spearman correlation) for CD4-CD8+ T cells and r = 0.86, p < 0.0001 for CD4+CD8+T cells.) CD4+CD8-T cells contained lower levels of re-stimulated cells (Fig. 4c). A similar pattern was observed when the proliferation level of re-stimulated PBMC was assessed (Fig. 5). Pigs receiving the highest dose of the vaccine had a proliferating recall response significantly higher than the control group. The expression of IFN-γ coincided with proliferating cells; the mean for all groups was 64.4% (standard deviation, 14.8) of proliferating cells also expressing IFN-γ.


A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs.

Borggren M, Nielsen J, Karlsson I, Dalgaard TS, Trebbien R, Williams JA, Fomsgaard A - Vaccine (2016)

T cell sub-population proliferation response to in vitro re-stimulation with influenza proteins. Pigs were vaccinated twice (day 0 and 21pv1) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). PBMC from the vaccinated pigs on day 35pv1 were cultured in vitro in the presence of recombinant influenza NP 2009, NP 1918, M1 1918 and HA 2009. After 6 days, the cells were stained with anti-CD3, -CD4, and -CD8 monoclonal antibodies and analyzed by flow cytometry. Three T cell subsets were identified based on their CD4 and CD8 expression: (a) CD4-CD8+, (b) CD4+CD8+ and (c) CD4+CD8- cells. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: **: p < 0.01; *: p < 0.05.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4940207&req=5

fig0025: T cell sub-population proliferation response to in vitro re-stimulation with influenza proteins. Pigs were vaccinated twice (day 0 and 21pv1) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). PBMC from the vaccinated pigs on day 35pv1 were cultured in vitro in the presence of recombinant influenza NP 2009, NP 1918, M1 1918 and HA 2009. After 6 days, the cells were stained with anti-CD3, -CD4, and -CD8 monoclonal antibodies and analyzed by flow cytometry. Three T cell subsets were identified based on their CD4 and CD8 expression: (a) CD4-CD8+, (b) CD4+CD8+ and (c) CD4+CD8- cells. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: **: p < 0.01; *: p < 0.05.
Mentions: The DNA vaccine elicited NP-, M1- and HA-specific CD4-CD8+ T cells and CD4+CD8+T cells producing IFN-γ (Fig. 4a and b). The IFN-γ response levels correlated with the DNA vaccine doses. Both vaccine-homologous and vaccine-heterologous NP (1918 and 2009, respectively) could re-stimulate the PBMC, and their respective IFN-γ responses correlated significantly (r = 0.78, p < 0.0001 (Spearman correlation) for CD4-CD8+ T cells and r = 0.86, p < 0.0001 for CD4+CD8+T cells.) CD4+CD8-T cells contained lower levels of re-stimulated cells (Fig. 4c). A similar pattern was observed when the proliferation level of re-stimulated PBMC was assessed (Fig. 5). Pigs receiving the highest dose of the vaccine had a proliferating recall response significantly higher than the control group. The expression of IFN-γ coincided with proliferating cells; the mean for all groups was 64.4% (standard deviation, 14.8) of proliferating cells also expressing IFN-γ.

Bottom Line: Both the antibody-mediated and the recall lymphocyte immune responses demonstrated high reactivity against vaccine-specific strains and cross-reactivity to vaccine-heterologous strains.The results suggest that polyvalent DNA influenza vaccination may provide a strong tool for broad protection against swine influenza strains threatening animal as well as public health.In addition, the needle-free administration technique used for this DNA vaccine will provide an easy and practical approach for the large-scale vaccination of swine.

View Article: PubMed Central - PubMed

Affiliation: Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Electronic address: mabo@ssi.dk.

No MeSH data available.


Related in: MedlinePlus