Limits...
A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs.

Borggren M, Nielsen J, Karlsson I, Dalgaard TS, Trebbien R, Williams JA, Fomsgaard A - Vaccine (2016)

Bottom Line: Both the antibody-mediated and the recall lymphocyte immune responses demonstrated high reactivity against vaccine-specific strains and cross-reactivity to vaccine-heterologous strains.The results suggest that polyvalent DNA influenza vaccination may provide a strong tool for broad protection against swine influenza strains threatening animal as well as public health.In addition, the needle-free administration technique used for this DNA vaccine will provide an easy and practical approach for the large-scale vaccination of swine.

View Article: PubMed Central - PubMed

Affiliation: Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Electronic address: mabo@ssi.dk.

No MeSH data available.


Related in: MedlinePlus

T cell sub-population IFN-γ response to in vitro re-stimulation with influenza proteins. Pigs were vaccinated twice (day 0 and 21pv1) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). PBMC from the vaccinated pigs on day 35pv1 were cultured in vitro in the presence of recombinant influenza NP 2009, NP 1918, M1 1918 and HA 2009. After 24 h, the cells were stained with anti-CD3, -CD4, -CD8 and -IFN-γ monoclonal antibodies and analyzed by flow cytometry. Three T cell subsets were identified based on their CD4 and CD8 expression: (a) CD4-CD8+, (b) CD4+CD8+ and (c) CD4+CD8- cells. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: ***: p < 0.001; **: p < 0.01; *: p < 0.05.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940207&req=5

fig0020: T cell sub-population IFN-γ response to in vitro re-stimulation with influenza proteins. Pigs were vaccinated twice (day 0 and 21pv1) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). PBMC from the vaccinated pigs on day 35pv1 were cultured in vitro in the presence of recombinant influenza NP 2009, NP 1918, M1 1918 and HA 2009. After 24 h, the cells were stained with anti-CD3, -CD4, -CD8 and -IFN-γ monoclonal antibodies and analyzed by flow cytometry. Three T cell subsets were identified based on their CD4 and CD8 expression: (a) CD4-CD8+, (b) CD4+CD8+ and (c) CD4+CD8- cells. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: ***: p < 0.001; **: p < 0.01; *: p < 0.05.

Mentions: The DNA vaccine elicited NP-, M1- and HA-specific CD4-CD8+ T cells and CD4+CD8+T cells producing IFN-γ (Fig. 4a and b). The IFN-γ response levels correlated with the DNA vaccine doses. Both vaccine-homologous and vaccine-heterologous NP (1918 and 2009, respectively) could re-stimulate the PBMC, and their respective IFN-γ responses correlated significantly (r = 0.78, p < 0.0001 (Spearman correlation) for CD4-CD8+ T cells and r = 0.86, p < 0.0001 for CD4+CD8+T cells.) CD4+CD8-T cells contained lower levels of re-stimulated cells (Fig. 4c). A similar pattern was observed when the proliferation level of re-stimulated PBMC was assessed (Fig. 5). Pigs receiving the highest dose of the vaccine had a proliferating recall response significantly higher than the control group. The expression of IFN-γ coincided with proliferating cells; the mean for all groups was 64.4% (standard deviation, 14.8) of proliferating cells also expressing IFN-γ.


A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs.

Borggren M, Nielsen J, Karlsson I, Dalgaard TS, Trebbien R, Williams JA, Fomsgaard A - Vaccine (2016)

T cell sub-population IFN-γ response to in vitro re-stimulation with influenza proteins. Pigs were vaccinated twice (day 0 and 21pv1) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). PBMC from the vaccinated pigs on day 35pv1 were cultured in vitro in the presence of recombinant influenza NP 2009, NP 1918, M1 1918 and HA 2009. After 24 h, the cells were stained with anti-CD3, -CD4, -CD8 and -IFN-γ monoclonal antibodies and analyzed by flow cytometry. Three T cell subsets were identified based on their CD4 and CD8 expression: (a) CD4-CD8+, (b) CD4+CD8+ and (c) CD4+CD8- cells. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: ***: p < 0.001; **: p < 0.01; *: p < 0.05.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940207&req=5

fig0020: T cell sub-population IFN-γ response to in vitro re-stimulation with influenza proteins. Pigs were vaccinated twice (day 0 and 21pv1) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). PBMC from the vaccinated pigs on day 35pv1 were cultured in vitro in the presence of recombinant influenza NP 2009, NP 1918, M1 1918 and HA 2009. After 24 h, the cells were stained with anti-CD3, -CD4, -CD8 and -IFN-γ monoclonal antibodies and analyzed by flow cytometry. Three T cell subsets were identified based on their CD4 and CD8 expression: (a) CD4-CD8+, (b) CD4+CD8+ and (c) CD4+CD8- cells. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: ***: p < 0.001; **: p < 0.01; *: p < 0.05.
Mentions: The DNA vaccine elicited NP-, M1- and HA-specific CD4-CD8+ T cells and CD4+CD8+T cells producing IFN-γ (Fig. 4a and b). The IFN-γ response levels correlated with the DNA vaccine doses. Both vaccine-homologous and vaccine-heterologous NP (1918 and 2009, respectively) could re-stimulate the PBMC, and their respective IFN-γ responses correlated significantly (r = 0.78, p < 0.0001 (Spearman correlation) for CD4-CD8+ T cells and r = 0.86, p < 0.0001 for CD4+CD8+T cells.) CD4+CD8-T cells contained lower levels of re-stimulated cells (Fig. 4c). A similar pattern was observed when the proliferation level of re-stimulated PBMC was assessed (Fig. 5). Pigs receiving the highest dose of the vaccine had a proliferating recall response significantly higher than the control group. The expression of IFN-γ coincided with proliferating cells; the mean for all groups was 64.4% (standard deviation, 14.8) of proliferating cells also expressing IFN-γ.

Bottom Line: Both the antibody-mediated and the recall lymphocyte immune responses demonstrated high reactivity against vaccine-specific strains and cross-reactivity to vaccine-heterologous strains.The results suggest that polyvalent DNA influenza vaccination may provide a strong tool for broad protection against swine influenza strains threatening animal as well as public health.In addition, the needle-free administration technique used for this DNA vaccine will provide an easy and practical approach for the large-scale vaccination of swine.

View Article: PubMed Central - PubMed

Affiliation: Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Electronic address: mabo@ssi.dk.

No MeSH data available.


Related in: MedlinePlus