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A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs.

Borggren M, Nielsen J, Karlsson I, Dalgaard TS, Trebbien R, Williams JA, Fomsgaard A - Vaccine (2016)

Bottom Line: Both the antibody-mediated and the recall lymphocyte immune responses demonstrated high reactivity against vaccine-specific strains and cross-reactivity to vaccine-heterologous strains.The results suggest that polyvalent DNA influenza vaccination may provide a strong tool for broad protection against swine influenza strains threatening animal as well as public health.In addition, the needle-free administration technique used for this DNA vaccine will provide an easy and practical approach for the large-scale vaccination of swine.

View Article: PubMed Central - PubMed

Affiliation: Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Electronic address: mabo@ssi.dk.

No MeSH data available.


Related in: MedlinePlus

Serum HI antibody titers in vaccinated pigs. Pigs were vaccinated twice (arrows) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). Vaccine-induced hemagglutination inhibition antibody responses in pig sera against (a) swine H1N1pdm09 and (b) swine H1N2 isolates were measured. The data presented are from one representative experiment out of two performed. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: ****: p < 0.0001; *: p < 0.05.
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fig0010: Serum HI antibody titers in vaccinated pigs. Pigs were vaccinated twice (arrows) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). Vaccine-induced hemagglutination inhibition antibody responses in pig sera against (a) swine H1N1pdm09 and (b) swine H1N2 isolates were measured. The data presented are from one representative experiment out of two performed. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: ****: p < 0.0001; *: p < 0.05.

Mentions: Vaccinated pigs had vaccine-induced serum HI antibodies that were cross-reactive against two swine virus strains, H1N1pdm09 and H1N2, which were heterologous to the vaccine genes (Fig. 2). The HI antibody levels were significantly higher in the group vaccinated with the highest dose of DNA than in the control group after day 28pv1. The HI titers obtained with the two different virus strains correlated significantly with each other (Spearman correlation, r = 0.50, p < 0.0001).


A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs.

Borggren M, Nielsen J, Karlsson I, Dalgaard TS, Trebbien R, Williams JA, Fomsgaard A - Vaccine (2016)

Serum HI antibody titers in vaccinated pigs. Pigs were vaccinated twice (arrows) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). Vaccine-induced hemagglutination inhibition antibody responses in pig sera against (a) swine H1N1pdm09 and (b) swine H1N2 isolates were measured. The data presented are from one representative experiment out of two performed. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: ****: p < 0.0001; *: p < 0.05.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940207&req=5

fig0010: Serum HI antibody titers in vaccinated pigs. Pigs were vaccinated twice (arrows) i.d. with needle-free delivery with 200 μg (n = 6), 800 μg (n = 6) or 1972 μg (n = 5) DNA, or not DNA vaccinated at all (n = 5). Vaccine-induced hemagglutination inhibition antibody responses in pig sera against (a) swine H1N1pdm09 and (b) swine H1N2 isolates were measured. The data presented are from one representative experiment out of two performed. Error bars indicate the mean ± SEM, and significant differences from the no-vaccine control group are indicated by: ****: p < 0.0001; *: p < 0.05.
Mentions: Vaccinated pigs had vaccine-induced serum HI antibodies that were cross-reactive against two swine virus strains, H1N1pdm09 and H1N2, which were heterologous to the vaccine genes (Fig. 2). The HI antibody levels were significantly higher in the group vaccinated with the highest dose of DNA than in the control group after day 28pv1. The HI titers obtained with the two different virus strains correlated significantly with each other (Spearman correlation, r = 0.50, p < 0.0001).

Bottom Line: Both the antibody-mediated and the recall lymphocyte immune responses demonstrated high reactivity against vaccine-specific strains and cross-reactivity to vaccine-heterologous strains.The results suggest that polyvalent DNA influenza vaccination may provide a strong tool for broad protection against swine influenza strains threatening animal as well as public health.In addition, the needle-free administration technique used for this DNA vaccine will provide an easy and practical approach for the large-scale vaccination of swine.

View Article: PubMed Central - PubMed

Affiliation: Virus Research and Development Laboratory, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Electronic address: mabo@ssi.dk.

No MeSH data available.


Related in: MedlinePlus