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Muscular invasion by oral squamous cell carcinoma of the posterior mandibular alveolar ridge is associated with cervical lymph node metastasis.

Min SK, Myoung H, Lee JH, Kim MJ - J Korean Assoc Oral Maxillofac Surg (2016)

Bottom Line: Cervical lymph node metastasis and masticator space invasion had a negative effect on overall survival.No lymphatic vessels were identified near the tumor invasion front within the mandible.In contrast, lymphatic vessels were identified near the front of tumor invasion in the muscles.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea.

ABSTRACT

Objectives: To assess the association between muscle invasion by oral squamous cell carcinoma of the posterior mandibular alveolar ridge and cervical lymph node metastasis on the basis of preoperative magnetic resonance imaging (MRI).

Materials and methods: Twenty-six patients with oral squamous cell carcinoma of the posterior mandibular alveolar ridge were evaluated by MRI. The associations between cervical lymph node metastasis and independent factors evaluated by MRI were analyzed. Overall survival was also analyzed in this manner. Representative biopsy specimens were stained with anti-podoplanin and anti-CD34 antibodies.

Results: Mylohyoid muscle invasion was associated with cervical lymph node metastasis. A combinational factor of mylohyoid and/or buccinator muscle invasion was also associated with cervical lymph node metastasis. Cervical lymph node metastasis and masticator space invasion had a negative effect on overall survival. No lymphatic vessels were identified near the tumor invasion front within the mandible. In contrast, lymphatic vessels were identified near the front of tumor invasion in the muscles.

Conclusion: This study demonstrates an association between muscular invasion by oral squamous cell carcinoma of the posterior mandibular alveolar ridge and cervical lymph node metastasis.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical stainings of sections from representative specimens are shown. No lymphatic vessels are identified in the tumor front within the marrow space of the mandible (A), while lymphatic vessels (arrows) are present at the submocosa overlying the mandible (B). Both Fig. 2. A and 2. B are different areas of the same section stained with anti-podoplanin antibody (A: ×100, B: ×200). With no evidence of lymphatic vessels near the bone marrow invasive tumor front in the anti-podoplanin-stained section (C) (×100), the capillary structures are variously stained by anti-CD34 antibody in a serial section (D) (×100). In contrast with the bone marrow, muscles attached to the mandible contain numerous lymphatic vessels (arrows) (E), thus when the tumor invades the muscles, they have close access to the lymphatic vessels (F). Fig. 2. E and 2. F stained with anti-podoplanin antibody (E: ×200, F: ×100).
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Figure 2: Immunohistochemical stainings of sections from representative specimens are shown. No lymphatic vessels are identified in the tumor front within the marrow space of the mandible (A), while lymphatic vessels (arrows) are present at the submocosa overlying the mandible (B). Both Fig. 2. A and 2. B are different areas of the same section stained with anti-podoplanin antibody (A: ×100, B: ×200). With no evidence of lymphatic vessels near the bone marrow invasive tumor front in the anti-podoplanin-stained section (C) (×100), the capillary structures are variously stained by anti-CD34 antibody in a serial section (D) (×100). In contrast with the bone marrow, muscles attached to the mandible contain numerous lymphatic vessels (arrows) (E), thus when the tumor invades the muscles, they have close access to the lymphatic vessels (F). Fig. 2. E and 2. F stained with anti-podoplanin antibody (E: ×200, F: ×100).

Mentions: Podoplanin is a lymphatic endothelial cell-specific marker, while CD34 is expressed in blood vessel endothelial cells15. In the bone marrow, no lymphatic vessels were identified within or near the tumor by anti-podoplanin antibody staining.(Fig. 2. A) However, podoplanin-expressing endothelial cells were present in the submucosal connective tissue.(Fig. 2. B) Tubular structures present in the bone marrow were stained by anti-CD34 antibody, demonstrating its vascularity.(Fig. 2. C, 2. D) Lymphatic vessels were identified in muscles attached to the mandible and near the tumor invasion fronts within these muscles.(Fig. 2. E, 2. F)


Muscular invasion by oral squamous cell carcinoma of the posterior mandibular alveolar ridge is associated with cervical lymph node metastasis.

Min SK, Myoung H, Lee JH, Kim MJ - J Korean Assoc Oral Maxillofac Surg (2016)

Immunohistochemical stainings of sections from representative specimens are shown. No lymphatic vessels are identified in the tumor front within the marrow space of the mandible (A), while lymphatic vessels (arrows) are present at the submocosa overlying the mandible (B). Both Fig. 2. A and 2. B are different areas of the same section stained with anti-podoplanin antibody (A: ×100, B: ×200). With no evidence of lymphatic vessels near the bone marrow invasive tumor front in the anti-podoplanin-stained section (C) (×100), the capillary structures are variously stained by anti-CD34 antibody in a serial section (D) (×100). In contrast with the bone marrow, muscles attached to the mandible contain numerous lymphatic vessels (arrows) (E), thus when the tumor invades the muscles, they have close access to the lymphatic vessels (F). Fig. 2. E and 2. F stained with anti-podoplanin antibody (E: ×200, F: ×100).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940197&req=5

Figure 2: Immunohistochemical stainings of sections from representative specimens are shown. No lymphatic vessels are identified in the tumor front within the marrow space of the mandible (A), while lymphatic vessels (arrows) are present at the submocosa overlying the mandible (B). Both Fig. 2. A and 2. B are different areas of the same section stained with anti-podoplanin antibody (A: ×100, B: ×200). With no evidence of lymphatic vessels near the bone marrow invasive tumor front in the anti-podoplanin-stained section (C) (×100), the capillary structures are variously stained by anti-CD34 antibody in a serial section (D) (×100). In contrast with the bone marrow, muscles attached to the mandible contain numerous lymphatic vessels (arrows) (E), thus when the tumor invades the muscles, they have close access to the lymphatic vessels (F). Fig. 2. E and 2. F stained with anti-podoplanin antibody (E: ×200, F: ×100).
Mentions: Podoplanin is a lymphatic endothelial cell-specific marker, while CD34 is expressed in blood vessel endothelial cells15. In the bone marrow, no lymphatic vessels were identified within or near the tumor by anti-podoplanin antibody staining.(Fig. 2. A) However, podoplanin-expressing endothelial cells were present in the submucosal connective tissue.(Fig. 2. B) Tubular structures present in the bone marrow were stained by anti-CD34 antibody, demonstrating its vascularity.(Fig. 2. C, 2. D) Lymphatic vessels were identified in muscles attached to the mandible and near the tumor invasion fronts within these muscles.(Fig. 2. E, 2. F)

Bottom Line: Cervical lymph node metastasis and masticator space invasion had a negative effect on overall survival.No lymphatic vessels were identified near the tumor invasion front within the mandible.In contrast, lymphatic vessels were identified near the front of tumor invasion in the muscles.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea.

ABSTRACT

Objectives: To assess the association between muscle invasion by oral squamous cell carcinoma of the posterior mandibular alveolar ridge and cervical lymph node metastasis on the basis of preoperative magnetic resonance imaging (MRI).

Materials and methods: Twenty-six patients with oral squamous cell carcinoma of the posterior mandibular alveolar ridge were evaluated by MRI. The associations between cervical lymph node metastasis and independent factors evaluated by MRI were analyzed. Overall survival was also analyzed in this manner. Representative biopsy specimens were stained with anti-podoplanin and anti-CD34 antibodies.

Results: Mylohyoid muscle invasion was associated with cervical lymph node metastasis. A combinational factor of mylohyoid and/or buccinator muscle invasion was also associated with cervical lymph node metastasis. Cervical lymph node metastasis and masticator space invasion had a negative effect on overall survival. No lymphatic vessels were identified near the tumor invasion front within the mandible. In contrast, lymphatic vessels were identified near the front of tumor invasion in the muscles.

Conclusion: This study demonstrates an association between muscular invasion by oral squamous cell carcinoma of the posterior mandibular alveolar ridge and cervical lymph node metastasis.

No MeSH data available.


Related in: MedlinePlus