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The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases BloodPressure In Hypertensive Rats

View Article: PubMed Central - PubMed

ABSTRACT

Background: Labdane-type diterpenes induce lower blood pressure via relaxation ofvascular smooth muscle; however, there are no studies describing the effectsof labdanes in hypertensive rats.

Objective: The present study was designed to investigate the cardiovascular actions ofthe labdane-type diterpene ent-3-acetoxy-labda-8(17), 13-dien-15-oic acid(labda-15-oic acid) in two-kidney 1 clip (2K-1C) renal hypertension.

Methods: Vascular reactivity experiments were performed in aortic rings isolated from2K-1C and normotensive (2K) male Wistar rats. Nitrate/nitrite (NOx)measurement was performed in aortas by colorimetric assay. Blood pressuremeasurements were performed in conscious rats.

Results: Labda-15-oic acid (0.1-300 µmol/l) and forskolin (0.1nmol/l - 1 µmol/l) relaxed endothelium-intact andendothelium-denuded aortas from both 2K-1C and 2K rats. Labda-15-oic acidwas more effective at inducing relaxation in endothelium-intact aortas from2K pre-contracted with phenylephrine when compared to theendothelium-denuded ones. Forskolin was more potent than labda-15-oic acidat inducing vascular relaxation in arteries from both 2K and 2K-1C rats.Labda-15-oic acid-induced increase in NOx levels was lower in arteries from2K-1C rats when compared to 2K rats. Intravenous administration oflabda-15-oic acid (0.3-3 mg/kg) or forskolin (0.1-1 mg/kg) inducedhypotension in conscious 2K-1C and 2K rats.

Conclusion: The present findings show that labda-15-oic acid induces vascular relaxationand hypotension in hypertensive rats.

No MeSH data available.


Effect of labda-15-oic acid on nitrate levels in endothelium-intactaortic rings from 2K and 2K-1C rats. Each bar represents the mean± S.E.M. of 6 to 8 independent preparations. *Compared withbasal values for 2K rats; #Compared with basal values for 2K-1Crats; ƒCompared with stimulation with labda-15-oic acidin 2K-1C rats (p < 0.05, ANOVA followed by Newman-Keuls multiplecomparison test).
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f6: Effect of labda-15-oic acid on nitrate levels in endothelium-intactaortic rings from 2K and 2K-1C rats. Each bar represents the mean± S.E.M. of 6 to 8 independent preparations. *Compared withbasal values for 2K rats; #Compared with basal values for 2K-1Crats; ƒCompared with stimulation with labda-15-oic acidin 2K-1C rats (p < 0.05, ANOVA followed by Newman-Keuls multiplecomparison test).

Mentions: Figure 6 show that NOx basal levels inaortas from 2K-1C rats are lower than those found in aortas from 2K rats.Labda-15-oic acid induced nitrate generation in endothelium-intact aortas fromboth 2K-1C and 2K rats. Labda-15-oic acid-induced nitrate generation was lowerin arteries from 2K-1C rats when compared to 2K rats (Figure 6).


The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases BloodPressure In Hypertensive Rats
Effect of labda-15-oic acid on nitrate levels in endothelium-intactaortic rings from 2K and 2K-1C rats. Each bar represents the mean± S.E.M. of 6 to 8 independent preparations. *Compared withbasal values for 2K rats; #Compared with basal values for 2K-1Crats; ƒCompared with stimulation with labda-15-oic acidin 2K-1C rats (p < 0.05, ANOVA followed by Newman-Keuls multiplecomparison test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940147&req=5

f6: Effect of labda-15-oic acid on nitrate levels in endothelium-intactaortic rings from 2K and 2K-1C rats. Each bar represents the mean± S.E.M. of 6 to 8 independent preparations. *Compared withbasal values for 2K rats; #Compared with basal values for 2K-1Crats; ƒCompared with stimulation with labda-15-oic acidin 2K-1C rats (p < 0.05, ANOVA followed by Newman-Keuls multiplecomparison test).
Mentions: Figure 6 show that NOx basal levels inaortas from 2K-1C rats are lower than those found in aortas from 2K rats.Labda-15-oic acid induced nitrate generation in endothelium-intact aortas fromboth 2K-1C and 2K rats. Labda-15-oic acid-induced nitrate generation was lowerin arteries from 2K-1C rats when compared to 2K rats (Figure 6).

View Article: PubMed Central - PubMed

ABSTRACT

Background: Labdane-type diterpenes induce lower blood pressure via relaxation ofvascular smooth muscle; however, there are no studies describing the effectsof labdanes in hypertensive rats.

Objective: The present study was designed to investigate the cardiovascular actions ofthe labdane-type diterpene ent-3-acetoxy-labda-8(17), 13-dien-15-oic acid(labda-15-oic acid) in two-kidney 1 clip (2K-1C) renal hypertension.

Methods: Vascular reactivity experiments were performed in aortic rings isolated from2K-1C and normotensive (2K) male Wistar rats. Nitrate/nitrite (NOx)measurement was performed in aortas by colorimetric assay. Blood pressuremeasurements were performed in conscious rats.

Results: Labda-15-oic acid (0.1-300 &micro;mol/l) and forskolin (0.1nmol/l - 1 &micro;mol/l) relaxed endothelium-intact andendothelium-denuded aortas from both 2K-1C and 2K rats. Labda-15-oic acidwas more effective at inducing relaxation in endothelium-intact aortas from2K pre-contracted with phenylephrine when compared to theendothelium-denuded ones. Forskolin was more potent than labda-15-oic acidat inducing vascular relaxation in arteries from both 2K and 2K-1C rats.Labda-15-oic acid-induced increase in NOx levels was lower in arteries from2K-1C rats when compared to 2K rats. Intravenous administration oflabda-15-oic acid (0.3-3 mg/kg) or forskolin (0.1-1 mg/kg) inducedhypotension in conscious 2K-1C and 2K rats.

Conclusion: The present findings show that labda-15-oic acid induces vascular relaxationand hypotension in hypertensive rats.

No MeSH data available.