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The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases BloodPressure In Hypertensive Rats

View Article: PubMed Central - PubMed

ABSTRACT

Background: Labdane-type diterpenes induce lower blood pressure via relaxation ofvascular smooth muscle; however, there are no studies describing the effectsof labdanes in hypertensive rats.

Objective: The present study was designed to investigate the cardiovascular actions ofthe labdane-type diterpene ent-3-acetoxy-labda-8(17), 13-dien-15-oic acid(labda-15-oic acid) in two-kidney 1 clip (2K-1C) renal hypertension.

Methods: Vascular reactivity experiments were performed in aortic rings isolated from2K-1C and normotensive (2K) male Wistar rats. Nitrate/nitrite (NOx)measurement was performed in aortas by colorimetric assay. Blood pressuremeasurements were performed in conscious rats.

Results: Labda-15-oic acid (0.1-300 µmol/l) and forskolin (0.1nmol/l - 1 µmol/l) relaxed endothelium-intact andendothelium-denuded aortas from both 2K-1C and 2K rats. Labda-15-oic acidwas more effective at inducing relaxation in endothelium-intact aortas from2K pre-contracted with phenylephrine when compared to theendothelium-denuded ones. Forskolin was more potent than labda-15-oic acidat inducing vascular relaxation in arteries from both 2K and 2K-1C rats.Labda-15-oic acid-induced increase in NOx levels was lower in arteries from2K-1C rats when compared to 2K rats. Intravenous administration oflabda-15-oic acid (0.3-3 mg/kg) or forskolin (0.1-1 mg/kg) inducedhypotension in conscious 2K-1C and 2K rats.

Conclusion: The present findings show that labda-15-oic acid induces vascular relaxationand hypotension in hypertensive rats.

No MeSH data available.


Related in: MedlinePlus

Representative traces of the hypotensive action displayed bylabda-15-oic acid (0.3 – 3 mg/kg) and forskolin (0.1 – 1 mg/kg) onconscious 2K and 2K-1C rats. Traces represent the mean values of themaximal decrease in mean arterial pressure of 5 to 6 animals.
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f4: Representative traces of the hypotensive action displayed bylabda-15-oic acid (0.3 – 3 mg/kg) and forskolin (0.1 – 1 mg/kg) onconscious 2K and 2K-1C rats. Traces represent the mean values of themaximal decrease in mean arterial pressure of 5 to 6 animals.

Mentions: Figure 4 shows representative tracings forthe effect of labda-15-oic acid and forskolin on blood pressure of 2K and 2K-1Crats. The maximal variation in MAP induced by labda-15-oic acid and forskolin inconscious 2K-1C and 2K rats is presented in Figure5. A bolus injection of labda-15-oic acid or forskolin produced adecrease in MAP in conscious 2K-1C and 2K rats. The MAP values returned to basallevels after injection of labda-15-oic acid. On the other hand, MAP values didnot return to basal levels after administration of forskolin at 1 mg/kg (Figure 5). Labda-15-oic acid induced a morepronounced fall in blood pressure in 2K when compared to 2K-1C rats. On theother hand, forskolin was found to be more effective at inducing decrease in MAPin 2K-1C when compared to 2K rats (Figure5). Values of blood pressure before and after drug administration aredescribed in Table 3.


The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases BloodPressure In Hypertensive Rats
Representative traces of the hypotensive action displayed bylabda-15-oic acid (0.3 – 3 mg/kg) and forskolin (0.1 – 1 mg/kg) onconscious 2K and 2K-1C rats. Traces represent the mean values of themaximal decrease in mean arterial pressure of 5 to 6 animals.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940147&req=5

f4: Representative traces of the hypotensive action displayed bylabda-15-oic acid (0.3 – 3 mg/kg) and forskolin (0.1 – 1 mg/kg) onconscious 2K and 2K-1C rats. Traces represent the mean values of themaximal decrease in mean arterial pressure of 5 to 6 animals.
Mentions: Figure 4 shows representative tracings forthe effect of labda-15-oic acid and forskolin on blood pressure of 2K and 2K-1Crats. The maximal variation in MAP induced by labda-15-oic acid and forskolin inconscious 2K-1C and 2K rats is presented in Figure5. A bolus injection of labda-15-oic acid or forskolin produced adecrease in MAP in conscious 2K-1C and 2K rats. The MAP values returned to basallevels after injection of labda-15-oic acid. On the other hand, MAP values didnot return to basal levels after administration of forskolin at 1 mg/kg (Figure 5). Labda-15-oic acid induced a morepronounced fall in blood pressure in 2K when compared to 2K-1C rats. On theother hand, forskolin was found to be more effective at inducing decrease in MAPin 2K-1C when compared to 2K rats (Figure5). Values of blood pressure before and after drug administration aredescribed in Table 3.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Labdane-type diterpenes induce lower blood pressure via relaxation ofvascular smooth muscle; however, there are no studies describing the effectsof labdanes in hypertensive rats.

Objective: The present study was designed to investigate the cardiovascular actions ofthe labdane-type diterpene ent-3-acetoxy-labda-8(17), 13-dien-15-oic acid(labda-15-oic acid) in two-kidney 1 clip (2K-1C) renal hypertension.

Methods: Vascular reactivity experiments were performed in aortic rings isolated from2K-1C and normotensive (2K) male Wistar rats. Nitrate/nitrite (NOx)measurement was performed in aortas by colorimetric assay. Blood pressuremeasurements were performed in conscious rats.

Results: Labda-15-oic acid (0.1-300 µmol/l) and forskolin (0.1nmol/l - 1 µmol/l) relaxed endothelium-intact andendothelium-denuded aortas from both 2K-1C and 2K rats. Labda-15-oic acidwas more effective at inducing relaxation in endothelium-intact aortas from2K pre-contracted with phenylephrine when compared to theendothelium-denuded ones. Forskolin was more potent than labda-15-oic acidat inducing vascular relaxation in arteries from both 2K and 2K-1C rats.Labda-15-oic acid-induced increase in NOx levels was lower in arteries from2K-1C rats when compared to 2K rats. Intravenous administration oflabda-15-oic acid (0.3-3 mg/kg) or forskolin (0.1-1 mg/kg) inducedhypotension in conscious 2K-1C and 2K rats.

Conclusion: The present findings show that labda-15-oic acid induces vascular relaxationand hypotension in hypertensive rats.

No MeSH data available.


Related in: MedlinePlus