Limits...
The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases BloodPressure In Hypertensive Rats

View Article: PubMed Central - PubMed

ABSTRACT

Background: Labdane-type diterpenes induce lower blood pressure via relaxation ofvascular smooth muscle; however, there are no studies describing the effectsof labdanes in hypertensive rats.

Objective: The present study was designed to investigate the cardiovascular actions ofthe labdane-type diterpene ent-3-acetoxy-labda-8(17), 13-dien-15-oic acid(labda-15-oic acid) in two-kidney 1 clip (2K-1C) renal hypertension.

Methods: Vascular reactivity experiments were performed in aortic rings isolated from2K-1C and normotensive (2K) male Wistar rats. Nitrate/nitrite (NOx)measurement was performed in aortas by colorimetric assay. Blood pressuremeasurements were performed in conscious rats.

Results: Labda-15-oic acid (0.1-300 µmol/l) and forskolin (0.1nmol/l - 1 µmol/l) relaxed endothelium-intact andendothelium-denuded aortas from both 2K-1C and 2K rats. Labda-15-oic acidwas more effective at inducing relaxation in endothelium-intact aortas from2K pre-contracted with phenylephrine when compared to theendothelium-denuded ones. Forskolin was more potent than labda-15-oic acidat inducing vascular relaxation in arteries from both 2K and 2K-1C rats.Labda-15-oic acid-induced increase in NOx levels was lower in arteries from2K-1C rats when compared to 2K rats. Intravenous administration oflabda-15-oic acid (0.3-3 mg/kg) or forskolin (0.1-1 mg/kg) inducedhypotension in conscious 2K-1C and 2K rats.

Conclusion: The present findings show that labda-15-oic acid induces vascular relaxationand hypotension in hypertensive rats.

No MeSH data available.


Related in: MedlinePlus

Relaxation responses induced by labda-15-oic acid on rat aorticrings. The relaxation induced by the labdane was studied onendothelium-intact (E+) and endothelium-denuded (E-) rat aorticrings contracted with either phenylephrine (0.1 µmol/l) orKCl (30 mmol/l). Steady tension was evoked by phenylephrine or KCland then labda-15-oic acid (0.1 - 300 µmol/l) was addedcumulatively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940147&req=5

f2: Relaxation responses induced by labda-15-oic acid on rat aorticrings. The relaxation induced by the labdane was studied onendothelium-intact (E+) and endothelium-denuded (E-) rat aorticrings contracted with either phenylephrine (0.1 µmol/l) orKCl (30 mmol/l). Steady tension was evoked by phenylephrine or KCland then labda-15-oic acid (0.1 - 300 µmol/l) was addedcumulatively.

Mentions: Labda-15-oic acid (Figure 1) reduced thesustained contractions induced by phenylephrine and KCl in endothelium-intactand endothelium-denuded aortas from both 2K-1C and 2K rats (Figure 2). The Emax values (percentage ofrelaxation) for the relaxant effect of labda-15-oic acid in endothelium-intactand endothelium-denuded rings pre-contracted with phenylephrine were notsignificantly different in aortas from 2K-1C and 2K rats (Table 2). However, differences were found in thepD2 values for labda-15-oic acid in endothelium-intact anddenuded rings pre-contracted with phenylephrine in aortas from 2K, but not 2K-1Crats. In the arteries pre-contracted with KCl, there was no difference betweenthe Emax and pD2 values for labda-15-oic acid inendothelium-intact or denuded rings from both 2K-1C and 2K rats (Table 2). The Emax andpD2 values for labda-15-oic acid in the rings pre-contracted withKCl were not different from those found in phenylephrine-pre-contracted ringsfrom both 2K-1C and 2K rats.


The Labdane Ent-3-Acetoxy-Labda-8(17), 13-Dien-15-Oic Decreases BloodPressure In Hypertensive Rats
Relaxation responses induced by labda-15-oic acid on rat aorticrings. The relaxation induced by the labdane was studied onendothelium-intact (E+) and endothelium-denuded (E-) rat aorticrings contracted with either phenylephrine (0.1 µmol/l) orKCl (30 mmol/l). Steady tension was evoked by phenylephrine or KCland then labda-15-oic acid (0.1 - 300 µmol/l) was addedcumulatively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940147&req=5

f2: Relaxation responses induced by labda-15-oic acid on rat aorticrings. The relaxation induced by the labdane was studied onendothelium-intact (E+) and endothelium-denuded (E-) rat aorticrings contracted with either phenylephrine (0.1 µmol/l) orKCl (30 mmol/l). Steady tension was evoked by phenylephrine or KCland then labda-15-oic acid (0.1 - 300 µmol/l) was addedcumulatively.
Mentions: Labda-15-oic acid (Figure 1) reduced thesustained contractions induced by phenylephrine and KCl in endothelium-intactand endothelium-denuded aortas from both 2K-1C and 2K rats (Figure 2). The Emax values (percentage ofrelaxation) for the relaxant effect of labda-15-oic acid in endothelium-intactand endothelium-denuded rings pre-contracted with phenylephrine were notsignificantly different in aortas from 2K-1C and 2K rats (Table 2). However, differences were found in thepD2 values for labda-15-oic acid in endothelium-intact anddenuded rings pre-contracted with phenylephrine in aortas from 2K, but not 2K-1Crats. In the arteries pre-contracted with KCl, there was no difference betweenthe Emax and pD2 values for labda-15-oic acid inendothelium-intact or denuded rings from both 2K-1C and 2K rats (Table 2). The Emax andpD2 values for labda-15-oic acid in the rings pre-contracted withKCl were not different from those found in phenylephrine-pre-contracted ringsfrom both 2K-1C and 2K rats.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Labdane-type diterpenes induce lower blood pressure via relaxation ofvascular smooth muscle; however, there are no studies describing the effectsof labdanes in hypertensive rats.

Objective: The present study was designed to investigate the cardiovascular actions ofthe labdane-type diterpene ent-3-acetoxy-labda-8(17), 13-dien-15-oic acid(labda-15-oic acid) in two-kidney 1 clip (2K-1C) renal hypertension.

Methods: Vascular reactivity experiments were performed in aortic rings isolated from2K-1C and normotensive (2K) male Wistar rats. Nitrate/nitrite (NOx)measurement was performed in aortas by colorimetric assay. Blood pressuremeasurements were performed in conscious rats.

Results: Labda-15-oic acid (0.1-300 µmol/l) and forskolin (0.1nmol/l - 1 µmol/l) relaxed endothelium-intact andendothelium-denuded aortas from both 2K-1C and 2K rats. Labda-15-oic acidwas more effective at inducing relaxation in endothelium-intact aortas from2K pre-contracted with phenylephrine when compared to theendothelium-denuded ones. Forskolin was more potent than labda-15-oic acidat inducing vascular relaxation in arteries from both 2K and 2K-1C rats.Labda-15-oic acid-induced increase in NOx levels was lower in arteries from2K-1C rats when compared to 2K rats. Intravenous administration oflabda-15-oic acid (0.3-3 mg/kg) or forskolin (0.1-1 mg/kg) inducedhypotension in conscious 2K-1C and 2K rats.

Conclusion: The present findings show that labda-15-oic acid induces vascular relaxationand hypotension in hypertensive rats.

No MeSH data available.


Related in: MedlinePlus