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Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis‑induced acute renal injury.

Wang P, Wang W, Shi Q, Zhao L, Mei F, Li C, Zuo T, He X - Mol Med Rep (2016)

Bottom Line: Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects.The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting.These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 were measured with enzyme‑linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

No MeSH data available.


Related in: MedlinePlus

Levels of p-p38 in the kidney tissues at the 12 h time point. Western blot analysis was used to determine the protein levels. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin; p-, phosphorylated; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
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f6-mmr-14-02-1123: Levels of p-p38 in the kidney tissues at the 12 h time point. Western blot analysis was used to determine the protein levels. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin; p-, phosphorylated; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Mentions: The p38 MAPK signaling pathway can regulate the expression of NF-κB in numerous inflammatory diseases. Therefore, this study detected the effect of PF on the activation of p38 MAPKs in renal tissues using western blot analysis. As shown in the Fig. 6, the ANP group exhibited a higher ratio of p-p38/p38 compared with the sham-operated group. Furthermore, the ANP+PF group exhibited a significant decrease in the ratio of p-p38/p38 compared with the ANP group at the 12 h time point (P<0.05).


Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis‑induced acute renal injury.

Wang P, Wang W, Shi Q, Zhao L, Mei F, Li C, Zuo T, He X - Mol Med Rep (2016)

Levels of p-p38 in the kidney tissues at the 12 h time point. Western blot analysis was used to determine the protein levels. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin; p-, phosphorylated; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940107&req=5

f6-mmr-14-02-1123: Levels of p-p38 in the kidney tissues at the 12 h time point. Western blot analysis was used to determine the protein levels. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin; p-, phosphorylated; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
Mentions: The p38 MAPK signaling pathway can regulate the expression of NF-κB in numerous inflammatory diseases. Therefore, this study detected the effect of PF on the activation of p38 MAPKs in renal tissues using western blot analysis. As shown in the Fig. 6, the ANP group exhibited a higher ratio of p-p38/p38 compared with the sham-operated group. Furthermore, the ANP+PF group exhibited a significant decrease in the ratio of p-p38/p38 compared with the ANP group at the 12 h time point (P<0.05).

Bottom Line: Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects.The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting.These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 were measured with enzyme‑linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

No MeSH data available.


Related in: MedlinePlus