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Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis‑induced acute renal injury.

Wang P, Wang W, Shi Q, Zhao L, Mei F, Li C, Zuo T, He X - Mol Med Rep (2016)

Bottom Line: Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects.The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting.These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 were measured with enzyme‑linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry analysis of NF-κB and caspase-3 and TUNEL staining. The expression of (A) NF-κB and (B) caspase-3 were analyzed by immunohistochemistry and (C) TUNEL staining was performed to determine the number of apoptotic cells in the renal tissues (magnification, ×400). (D) The ratio of positively-stained cells was determined. *P<0.05 vs. the ANP group. NF-κB, nuclear factor-κB; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin.
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f4-mmr-14-02-1123: Immunohistochemistry analysis of NF-κB and caspase-3 and TUNEL staining. The expression of (A) NF-κB and (B) caspase-3 were analyzed by immunohistochemistry and (C) TUNEL staining was performed to determine the number of apoptotic cells in the renal tissues (magnification, ×400). (D) The ratio of positively-stained cells was determined. *P<0.05 vs. the ANP group. NF-κB, nuclear factor-κB; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin.

Mentions: In order to detect the degree of inflammation in renal tissues, immunostaining of NF-κB was measured in the kidney samples at the 12 h time point in each group. In the ANP+PF group, the expression of NF-κB was lower than in the ANP group (Fig. 4A). To identify the effects of PF on apoptotic changes in renal tissues, caspase-3 protein expression was detected with immunohistochemistry. A marked increase in caspase-3 staining was found in the nucleus in the ANP group. In the ANP+PF group the expression of caspase-3 was markedly decreased (Fig. 4B). To further confirm the apoptotic changes, numbers of TUNEL-positive cells were measured. The number of TUNEL-positive cells increased in the ANP group compared with those in sham-operated group. PF attenuated the increase of TUNEL-positive cells number in renal tissue (Fig. 4C). The ratios of positively stained cells were determined and it was shown that the number of cells expression NF-κB and caspase-3 were significantly decreased in the ANP+PF group, as compared with the ANP group (P<0.05; Fig. 4D). Furthermore, the numbers of TUNEL-positive cells were significantly decreased in the ANP+PF group, as compared with the ANP group (P<0.05; Fig. 4D).


Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis‑induced acute renal injury.

Wang P, Wang W, Shi Q, Zhao L, Mei F, Li C, Zuo T, He X - Mol Med Rep (2016)

Immunohistochemistry analysis of NF-κB and caspase-3 and TUNEL staining. The expression of (A) NF-κB and (B) caspase-3 were analyzed by immunohistochemistry and (C) TUNEL staining was performed to determine the number of apoptotic cells in the renal tissues (magnification, ×400). (D) The ratio of positively-stained cells was determined. *P<0.05 vs. the ANP group. NF-κB, nuclear factor-κB; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940107&req=5

f4-mmr-14-02-1123: Immunohistochemistry analysis of NF-κB and caspase-3 and TUNEL staining. The expression of (A) NF-κB and (B) caspase-3 were analyzed by immunohistochemistry and (C) TUNEL staining was performed to determine the number of apoptotic cells in the renal tissues (magnification, ×400). (D) The ratio of positively-stained cells was determined. *P<0.05 vs. the ANP group. NF-κB, nuclear factor-κB; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin.
Mentions: In order to detect the degree of inflammation in renal tissues, immunostaining of NF-κB was measured in the kidney samples at the 12 h time point in each group. In the ANP+PF group, the expression of NF-κB was lower than in the ANP group (Fig. 4A). To identify the effects of PF on apoptotic changes in renal tissues, caspase-3 protein expression was detected with immunohistochemistry. A marked increase in caspase-3 staining was found in the nucleus in the ANP group. In the ANP+PF group the expression of caspase-3 was markedly decreased (Fig. 4B). To further confirm the apoptotic changes, numbers of TUNEL-positive cells were measured. The number of TUNEL-positive cells increased in the ANP group compared with those in sham-operated group. PF attenuated the increase of TUNEL-positive cells number in renal tissue (Fig. 4C). The ratios of positively stained cells were determined and it was shown that the number of cells expression NF-κB and caspase-3 were significantly decreased in the ANP+PF group, as compared with the ANP group (P<0.05; Fig. 4D). Furthermore, the numbers of TUNEL-positive cells were significantly decreased in the ANP+PF group, as compared with the ANP group (P<0.05; Fig. 4D).

Bottom Line: Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects.The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting.These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 were measured with enzyme‑linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

No MeSH data available.


Related in: MedlinePlus