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Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis‑induced acute renal injury.

Wang P, Wang W, Shi Q, Zhao L, Mei F, Li C, Zuo T, He X - Mol Med Rep (2016)

Bottom Line: Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects.The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting.These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 were measured with enzyme‑linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

No MeSH data available.


Related in: MedlinePlus

Serum levels of AMY, LIPA, BUN and Cr in all groups. Serum levels of (A) AMY, (B) LIPA, (C) BUN and (D) Cr. Data are expressed as the mean ± standard deviation; n=8 in each group. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin; AMY, amylase; LIPA, lipase; BUN, blood urea nitrogen; Cr, creatine.
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f3-mmr-14-02-1123: Serum levels of AMY, LIPA, BUN and Cr in all groups. Serum levels of (A) AMY, (B) LIPA, (C) BUN and (D) Cr. Data are expressed as the mean ± standard deviation; n=8 in each group. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin; AMY, amylase; LIPA, lipase; BUN, blood urea nitrogen; Cr, creatine.

Mentions: Compared with the levels of AMY, LIPA, BUN and Cr in the shame-operated group, the levels in the ANP group were significantly increased at each time point (Fig. 3). Peak serum AMY level was observed at the 6 h time point in the ANP group and the peak serum LIPA, BUN and Cr levels were observed at the 12 h time point. In the ANP+PF groups the serum enzyme levels were significantly reduced at the corresponding time points compared with the ANP group (P<0.05).


Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis‑induced acute renal injury.

Wang P, Wang W, Shi Q, Zhao L, Mei F, Li C, Zuo T, He X - Mol Med Rep (2016)

Serum levels of AMY, LIPA, BUN and Cr in all groups. Serum levels of (A) AMY, (B) LIPA, (C) BUN and (D) Cr. Data are expressed as the mean ± standard deviation; n=8 in each group. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin; AMY, amylase; LIPA, lipase; BUN, blood urea nitrogen; Cr, creatine.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940107&req=5

f3-mmr-14-02-1123: Serum levels of AMY, LIPA, BUN and Cr in all groups. Serum levels of (A) AMY, (B) LIPA, (C) BUN and (D) Cr. Data are expressed as the mean ± standard deviation; n=8 in each group. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin; AMY, amylase; LIPA, lipase; BUN, blood urea nitrogen; Cr, creatine.
Mentions: Compared with the levels of AMY, LIPA, BUN and Cr in the shame-operated group, the levels in the ANP group were significantly increased at each time point (Fig. 3). Peak serum AMY level was observed at the 6 h time point in the ANP group and the peak serum LIPA, BUN and Cr levels were observed at the 12 h time point. In the ANP+PF groups the serum enzyme levels were significantly reduced at the corresponding time points compared with the ANP group (P<0.05).

Bottom Line: Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects.The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting.These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 were measured with enzyme‑linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

No MeSH data available.


Related in: MedlinePlus