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Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis‑induced acute renal injury.

Wang P, Wang W, Shi Q, Zhao L, Mei F, Li C, Zuo T, He X - Mol Med Rep (2016)

Bottom Line: Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects.The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting.These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 were measured with enzyme‑linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

No MeSH data available.


Related in: MedlinePlus

Morphological changes in pancreatic tissue at 12 h and the pancreatic histological score at different time points. Hematoxylin and eosin staining was used to examine the pathological changes (original magnification, ×200). (A) The sham-operated group, (B) the ANP group, (C) the PF group and (D) the pancreatic histological score at corresponding times. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin.
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f1-mmr-14-02-1123: Morphological changes in pancreatic tissue at 12 h and the pancreatic histological score at different time points. Hematoxylin and eosin staining was used to examine the pathological changes (original magnification, ×200). (A) The sham-operated group, (B) the ANP group, (C) the PF group and (D) the pancreatic histological score at corresponding times. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin.

Mentions: Representative pathological changes in pancreatic tissue are shown in the Fig. 1. Normal histological features of the pancreas were observed in the sham-operated group (Fig. 1A). In the ANP group, pancreatic edema, interstitial leukocyte infiltration, hemorrhage and necrosis were observed (Fig. 1B). In the ANP+100 mg/kg PF group, the severity of pancreatic histological injuries was significantly reduced compared with the ANP group (Fig. 1C). As shown in Fig. 1D the histopathological score in the pancreas was lower in the PF group compared with that in the ANP group at the corresponding time points (P<0.05).


Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitis‑induced acute renal injury.

Wang P, Wang W, Shi Q, Zhao L, Mei F, Li C, Zuo T, He X - Mol Med Rep (2016)

Morphological changes in pancreatic tissue at 12 h and the pancreatic histological score at different time points. Hematoxylin and eosin staining was used to examine the pathological changes (original magnification, ×200). (A) The sham-operated group, (B) the ANP group, (C) the PF group and (D) the pancreatic histological score at corresponding times. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940107&req=5

f1-mmr-14-02-1123: Morphological changes in pancreatic tissue at 12 h and the pancreatic histological score at different time points. Hematoxylin and eosin staining was used to examine the pathological changes (original magnification, ×200). (A) The sham-operated group, (B) the ANP group, (C) the PF group and (D) the pancreatic histological score at corresponding times. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the ANP group. SO, sham-operated; ANP, acute necrotizing pancreatitis; PF, paeoniflorin.
Mentions: Representative pathological changes in pancreatic tissue are shown in the Fig. 1. Normal histological features of the pancreas were observed in the sham-operated group (Fig. 1A). In the ANP group, pancreatic edema, interstitial leukocyte infiltration, hemorrhage and necrosis were observed (Fig. 1B). In the ANP+100 mg/kg PF group, the severity of pancreatic histological injuries was significantly reduced compared with the ANP group (Fig. 1C). As shown in Fig. 1D the histopathological score in the pancreas was lower in the PF group compared with that in the ANP group at the corresponding time points (P<0.05).

Bottom Line: Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects.The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting.These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Acute renal injury caused by acute necrotizing pancreatitis (ANP) is a common complication that is associated with a high rate of mortality. Paeoniflorin is the active ingredient of paeonia radix and exhibits a number of pharmacological effects, such as anti‑inflammatory, anticancer, analgesic and immunomodulatory effects. The present study detected the potential treatment effects of paeoniflorin on acute renal injury induced by ANP in a rat model. The optimal dose of paeoniflorin for preventing acute renal injury induced by ANP was determined. Then, the possible protective mechanism of paeoniflorin was investigated. The serum levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 were measured with enzyme‑linked immunosorbent assay kits. Renal inflammation and apoptosis were measured by immunohistochemistry and terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling assay. The expression of nitric oxide in kidney tissues was also evaluated. The p38 mitogen‑activated protein kinases (MAPKs) were measured by western blotting. The results shown that paeoniflorin may ameliorate acute renal injury following ANP in rats by inhibiting inflammatory responses and renal cell apoptosis. These effects may be associated with the p38MAPK and nuclear factor‑κB signal pathway.

No MeSH data available.


Related in: MedlinePlus