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Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation.

Fu X, Tong Z, Li Q, Niu Q, Zhang Z, Tong X, Tong L, Zhang X - Mol Med Rep (2016)

Bottom Line: The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers.In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells.These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China.

ABSTRACT
The peripheral nervous system has the potential for full regeneration following injury and recovery, predominantly controlled by Schwann cells (SCs). Therefore, obtaining a sufficient number of SCs in a short duration is crucial. In the present study, rat adipose‑derived stem cells (ADSCs) were isolated and cultured, following which characterization of the ADSCs was performed using flow cytometry. The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers. The multilineage differentiation potential of the ADSCs was assayed by determining the ability of the cells to differentiate into osteoblasts and adipocytes. Following this, the ADSCs were treated with a specific medium and differentiated into Schwann‑like cells. Immunofluorescence, western blot and reverse transcription‑quantitative polymerase chain reaction analyses showed that ~95% of the differentiated cells expressed glial fibrillary acidic protein, S100 and p75. In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells. These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

No MeSH data available.


Related in: MedlinePlus

Analysis of the proliferation of Schwann-like cells. (A) a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay was used to examine the growth rate of Schwann-like cell populations following differentiation for 1, 3, 5, 7, 9 and 11 days. Values are presented as the mean ± standard deviation of triplicate experiments. Images of mitosis of the Schwann-like cells in (B) prophase, (C) metaphase, (D) anaphase and (E) telophase. Scale bar=100 µm.
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f3-mmr-14-02-1187: Analysis of the proliferation of Schwann-like cells. (A) a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay was used to examine the growth rate of Schwann-like cell populations following differentiation for 1, 3, 5, 7, 9 and 11 days. Values are presented as the mean ± standard deviation of triplicate experiments. Images of mitosis of the Schwann-like cells in (B) prophase, (C) metaphase, (D) anaphase and (E) telophase. Scale bar=100 µm.

Mentions: In the present study, the viability and proliferation of the Schwann-like cells were analyzed using an MTT assay. As shown in Fig. 3A, during the first 1–3 days, the cells grew slowly. The rate of cell proliferation increased more rapidly from day 3, and this rapid growth rate continued until day 7. Between days 9 and 11, the cells continue to grow, however, the growth rate had declined. During the experiment, images were captured of mitosis in the Schwann-like cells in each period. The chromatin filament formed a spiral shape, resembling a loose knitting ball, during prophase of mitosis (Fig. 3B). During metaphase (Fig. 3C), the chromosomes arranged in order at the equator and, during anaphase (Fig. 3D), the two chromatids of each chromosome separated and moved in the opposite directions. The chromatids divided into two groups and the cells were elongated, exhibiting a dumbbell-like shape. In telophase (Fig. 3E), gradual unwinding of the chromatids was observed, with one cell dividing into two cells.


Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation.

Fu X, Tong Z, Li Q, Niu Q, Zhang Z, Tong X, Tong L, Zhang X - Mol Med Rep (2016)

Analysis of the proliferation of Schwann-like cells. (A) a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay was used to examine the growth rate of Schwann-like cell populations following differentiation for 1, 3, 5, 7, 9 and 11 days. Values are presented as the mean ± standard deviation of triplicate experiments. Images of mitosis of the Schwann-like cells in (B) prophase, (C) metaphase, (D) anaphase and (E) telophase. Scale bar=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940092&req=5

f3-mmr-14-02-1187: Analysis of the proliferation of Schwann-like cells. (A) a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay was used to examine the growth rate of Schwann-like cell populations following differentiation for 1, 3, 5, 7, 9 and 11 days. Values are presented as the mean ± standard deviation of triplicate experiments. Images of mitosis of the Schwann-like cells in (B) prophase, (C) metaphase, (D) anaphase and (E) telophase. Scale bar=100 µm.
Mentions: In the present study, the viability and proliferation of the Schwann-like cells were analyzed using an MTT assay. As shown in Fig. 3A, during the first 1–3 days, the cells grew slowly. The rate of cell proliferation increased more rapidly from day 3, and this rapid growth rate continued until day 7. Between days 9 and 11, the cells continue to grow, however, the growth rate had declined. During the experiment, images were captured of mitosis in the Schwann-like cells in each period. The chromatin filament formed a spiral shape, resembling a loose knitting ball, during prophase of mitosis (Fig. 3B). During metaphase (Fig. 3C), the chromosomes arranged in order at the equator and, during anaphase (Fig. 3D), the two chromatids of each chromosome separated and moved in the opposite directions. The chromatids divided into two groups and the cells were elongated, exhibiting a dumbbell-like shape. In telophase (Fig. 3E), gradual unwinding of the chromatids was observed, with one cell dividing into two cells.

Bottom Line: The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers.In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells.These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China.

ABSTRACT
The peripheral nervous system has the potential for full regeneration following injury and recovery, predominantly controlled by Schwann cells (SCs). Therefore, obtaining a sufficient number of SCs in a short duration is crucial. In the present study, rat adipose‑derived stem cells (ADSCs) were isolated and cultured, following which characterization of the ADSCs was performed using flow cytometry. The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers. The multilineage differentiation potential of the ADSCs was assayed by determining the ability of the cells to differentiate into osteoblasts and adipocytes. Following this, the ADSCs were treated with a specific medium and differentiated into Schwann‑like cells. Immunofluorescence, western blot and reverse transcription‑quantitative polymerase chain reaction analyses showed that ~95% of the differentiated cells expressed glial fibrillary acidic protein, S100 and p75. In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells. These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

No MeSH data available.


Related in: MedlinePlus