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Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation.

Fu X, Tong Z, Li Q, Niu Q, Zhang Z, Tong X, Tong L, Zhang X - Mol Med Rep (2016)

Bottom Line: The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers.In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells.These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China.

ABSTRACT
The peripheral nervous system has the potential for full regeneration following injury and recovery, predominantly controlled by Schwann cells (SCs). Therefore, obtaining a sufficient number of SCs in a short duration is crucial. In the present study, rat adipose‑derived stem cells (ADSCs) were isolated and cultured, following which characterization of the ADSCs was performed using flow cytometry. The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers. The multilineage differentiation potential of the ADSCs was assayed by determining the ability of the cells to differentiate into osteoblasts and adipocytes. Following this, the ADSCs were treated with a specific medium and differentiated into Schwann‑like cells. Immunofluorescence, western blot and reverse transcription‑quantitative polymerase chain reaction analyses showed that ~95% of the differentiated cells expressed glial fibrillary acidic protein, S100 and p75. In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells. These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

No MeSH data available.


Related in: MedlinePlus

ADSC differentiation into cells with an SC phenotype. (A) Cultured cells at the early stage of differentiation. (B) ADSCs differentiated into SC phenotype. Immunofluorescence staining of (C) GFAP, (D) S100 and (E) P75. Scale bar=100 µm. Cell nuclei were labeled with DAPI (blue). Detection of (F) GFAP, (G) S100 and (H) P75 proteins using western blot analysis. (I) Relative protein expression levels of GFAP, S100 and P75 (*P<0.05, vs. ADSCs). 1, ADSCs, 2, Schwann-like cells. (J) Detection of mRNA expression levels of GFAP, S100 and P75 using reverse transcription-quantitative polymerase chain reaction analysis (**P<0.01, vs. ADSCs). Values are presented as the mean ± standard deviation. SC, Schwann cell; ADSCs, adipose-derived stem cells; GFAP, glial fibrillary acidic protein.
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f2-mmr-14-02-1187: ADSC differentiation into cells with an SC phenotype. (A) Cultured cells at the early stage of differentiation. (B) ADSCs differentiated into SC phenotype. Immunofluorescence staining of (C) GFAP, (D) S100 and (E) P75. Scale bar=100 µm. Cell nuclei were labeled with DAPI (blue). Detection of (F) GFAP, (G) S100 and (H) P75 proteins using western blot analysis. (I) Relative protein expression levels of GFAP, S100 and P75 (*P<0.05, vs. ADSCs). 1, ADSCs, 2, Schwann-like cells. (J) Detection of mRNA expression levels of GFAP, S100 and P75 using reverse transcription-quantitative polymerase chain reaction analysis (**P<0.01, vs. ADSCs). Values are presented as the mean ± standard deviation. SC, Schwann cell; ADSCs, adipose-derived stem cells; GFAP, glial fibrillary acidic protein.

Mentions: The rat ADSCs were treated with SC-conditioned medium for 12 days. At the beginning of differentiation, the morphology of the ADSCs changed from a monolayer of large cells with a flat morphology to a small number of bipolar or tripolar spindle-like shaped cells (Fig. 2A). As the cells continued to proliferate, the density of cells was markedly increased (Fig. 2B). The results of the immunofluorescence (Fig. 2C–E), western blot (Table I, Fig. 2F–I) and RT-qPCR (Fig. 2J) analyses showed that almost all the differentiated ADSCs were positive for GFAP, S100 and P75. These characteristics of differentiated ADSCs were similar to those of SCs.


Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation.

Fu X, Tong Z, Li Q, Niu Q, Zhang Z, Tong X, Tong L, Zhang X - Mol Med Rep (2016)

ADSC differentiation into cells with an SC phenotype. (A) Cultured cells at the early stage of differentiation. (B) ADSCs differentiated into SC phenotype. Immunofluorescence staining of (C) GFAP, (D) S100 and (E) P75. Scale bar=100 µm. Cell nuclei were labeled with DAPI (blue). Detection of (F) GFAP, (G) S100 and (H) P75 proteins using western blot analysis. (I) Relative protein expression levels of GFAP, S100 and P75 (*P<0.05, vs. ADSCs). 1, ADSCs, 2, Schwann-like cells. (J) Detection of mRNA expression levels of GFAP, S100 and P75 using reverse transcription-quantitative polymerase chain reaction analysis (**P<0.01, vs. ADSCs). Values are presented as the mean ± standard deviation. SC, Schwann cell; ADSCs, adipose-derived stem cells; GFAP, glial fibrillary acidic protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940092&req=5

f2-mmr-14-02-1187: ADSC differentiation into cells with an SC phenotype. (A) Cultured cells at the early stage of differentiation. (B) ADSCs differentiated into SC phenotype. Immunofluorescence staining of (C) GFAP, (D) S100 and (E) P75. Scale bar=100 µm. Cell nuclei were labeled with DAPI (blue). Detection of (F) GFAP, (G) S100 and (H) P75 proteins using western blot analysis. (I) Relative protein expression levels of GFAP, S100 and P75 (*P<0.05, vs. ADSCs). 1, ADSCs, 2, Schwann-like cells. (J) Detection of mRNA expression levels of GFAP, S100 and P75 using reverse transcription-quantitative polymerase chain reaction analysis (**P<0.01, vs. ADSCs). Values are presented as the mean ± standard deviation. SC, Schwann cell; ADSCs, adipose-derived stem cells; GFAP, glial fibrillary acidic protein.
Mentions: The rat ADSCs were treated with SC-conditioned medium for 12 days. At the beginning of differentiation, the morphology of the ADSCs changed from a monolayer of large cells with a flat morphology to a small number of bipolar or tripolar spindle-like shaped cells (Fig. 2A). As the cells continued to proliferate, the density of cells was markedly increased (Fig. 2B). The results of the immunofluorescence (Fig. 2C–E), western blot (Table I, Fig. 2F–I) and RT-qPCR (Fig. 2J) analyses showed that almost all the differentiated ADSCs were positive for GFAP, S100 and P75. These characteristics of differentiated ADSCs were similar to those of SCs.

Bottom Line: The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers.In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells.These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China.

ABSTRACT
The peripheral nervous system has the potential for full regeneration following injury and recovery, predominantly controlled by Schwann cells (SCs). Therefore, obtaining a sufficient number of SCs in a short duration is crucial. In the present study, rat adipose‑derived stem cells (ADSCs) were isolated and cultured, following which characterization of the ADSCs was performed using flow cytometry. The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers. The multilineage differentiation potential of the ADSCs was assayed by determining the ability of the cells to differentiate into osteoblasts and adipocytes. Following this, the ADSCs were treated with a specific medium and differentiated into Schwann‑like cells. Immunofluorescence, western blot and reverse transcription‑quantitative polymerase chain reaction analyses showed that ~95% of the differentiated cells expressed glial fibrillary acidic protein, S100 and p75. In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells. These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

No MeSH data available.


Related in: MedlinePlus