Limits...
Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation.

Fu X, Tong Z, Li Q, Niu Q, Zhang Z, Tong X, Tong L, Zhang X - Mol Med Rep (2016)

Bottom Line: The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers.In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells.These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China.

ABSTRACT
The peripheral nervous system has the potential for full regeneration following injury and recovery, predominantly controlled by Schwann cells (SCs). Therefore, obtaining a sufficient number of SCs in a short duration is crucial. In the present study, rat adipose‑derived stem cells (ADSCs) were isolated and cultured, following which characterization of the ADSCs was performed using flow cytometry. The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers. The multilineage differentiation potential of the ADSCs was assayed by determining the ability of the cells to differentiate into osteoblasts and adipocytes. Following this, the ADSCs were treated with a specific medium and differentiated into Schwann‑like cells. Immunofluorescence, western blot and reverse transcription‑quantitative polymerase chain reaction analyses showed that ~95% of the differentiated cells expressed glial fibrillary acidic protein, S100 and p75. In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells. These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

No MeSH data available.


Related in: MedlinePlus

Isolation and identification of ADSCs. (A) Primary culture of ADSCs within 24 h; (B) ADSCs in the fourth passage; (C) ADSCs following 10 passages. Scale bar=100 µm. ADSCs were stained using (D) Alizarin Red and (E) Oil-Red O (magnification, ×400). Scale bar=100 µm. (F–H) Flow cytometric analysis of ADSCs. ADSCs, adipose-derived stem cells. FITC, fluorescein isothiocyanate; PE, phycoerythrin; APC, allophycocyanin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940092&req=5

f1-mmr-14-02-1187: Isolation and identification of ADSCs. (A) Primary culture of ADSCs within 24 h; (B) ADSCs in the fourth passage; (C) ADSCs following 10 passages. Scale bar=100 µm. ADSCs were stained using (D) Alizarin Red and (E) Oil-Red O (magnification, ×400). Scale bar=100 µm. (F–H) Flow cytometric analysis of ADSCs. ADSCs, adipose-derived stem cells. FITC, fluorescein isothiocyanate; PE, phycoerythrin; APC, allophycocyanin.

Mentions: After 24 h primary culture, the rat ADSCs had adhered with a short spindle or polygonal shape (Fig. 1A), whereas at 72 h, the cell density was increased, and fibroblast-like colonies had formed. After 1 week, the ADSCs exhibited a uniformly aligned monolayer of fibroblast-like cells, with a swirl or parallel growth style (Fig. 1B). Between the first and fifth passages, the growth of the ADSCs was marked. However, the cells appeared irregular and larger in size at passage 10, and showed reduced proliferation (Fig. 1C).


Induction of adipose-derived stem cells into Schwann-like cells and observation of Schwann-like cell proliferation.

Fu X, Tong Z, Li Q, Niu Q, Zhang Z, Tong X, Tong L, Zhang X - Mol Med Rep (2016)

Isolation and identification of ADSCs. (A) Primary culture of ADSCs within 24 h; (B) ADSCs in the fourth passage; (C) ADSCs following 10 passages. Scale bar=100 µm. ADSCs were stained using (D) Alizarin Red and (E) Oil-Red O (magnification, ×400). Scale bar=100 µm. (F–H) Flow cytometric analysis of ADSCs. ADSCs, adipose-derived stem cells. FITC, fluorescein isothiocyanate; PE, phycoerythrin; APC, allophycocyanin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940092&req=5

f1-mmr-14-02-1187: Isolation and identification of ADSCs. (A) Primary culture of ADSCs within 24 h; (B) ADSCs in the fourth passage; (C) ADSCs following 10 passages. Scale bar=100 µm. ADSCs were stained using (D) Alizarin Red and (E) Oil-Red O (magnification, ×400). Scale bar=100 µm. (F–H) Flow cytometric analysis of ADSCs. ADSCs, adipose-derived stem cells. FITC, fluorescein isothiocyanate; PE, phycoerythrin; APC, allophycocyanin.
Mentions: After 24 h primary culture, the rat ADSCs had adhered with a short spindle or polygonal shape (Fig. 1A), whereas at 72 h, the cell density was increased, and fibroblast-like colonies had formed. After 1 week, the ADSCs exhibited a uniformly aligned monolayer of fibroblast-like cells, with a swirl or parallel growth style (Fig. 1B). Between the first and fifth passages, the growth of the ADSCs was marked. However, the cells appeared irregular and larger in size at passage 10, and showed reduced proliferation (Fig. 1C).

Bottom Line: The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers.In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells.These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China.

ABSTRACT
The peripheral nervous system has the potential for full regeneration following injury and recovery, predominantly controlled by Schwann cells (SCs). Therefore, obtaining a sufficient number of SCs in a short duration is crucial. In the present study, rat adipose‑derived stem cells (ADSCs) were isolated and cultured, following which characterization of the ADSCs was performed using flow cytometry. The results showed that the cells were positive for the CD29 and CD44 markers, and negative for the CD31, CD45, CD49 and CD106 markers. The multilineage differentiation potential of the ADSCs was assayed by determining the ability of the cells to differentiate into osteoblasts and adipocytes. Following this, the ADSCs were treated with a specific medium and differentiated into Schwann‑like cells. Immunofluorescence, western blot and reverse transcription‑quantitative polymerase chain reaction analyses showed that ~95% of the differentiated cells expressed glial fibrillary acidic protein, S100 and p75. In addition, the present study found that a substantial number of SCs can be produced in a short duration via the mitotic feature of Schwann‑like cells. These data indicated that Schwann‑like cells derived from ADSCs can undergo mitotic proliferation, which may be beneficial for the treatment of peripheral nerve injury in the future.

No MeSH data available.


Related in: MedlinePlus