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Screening targeted testis‑specific genes for molecular assessment of aberrant sperm quality.

Liu XX, Shen XF, Liu FJ - Mol Med Rep (2016)

Bottom Line: Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility.These genes were functionally associated with the reproduction process.Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Central Laboratory, Yantai Yu Huang Ding Hospital, Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility. Genes and gene products associated with teratospermia may serve as targeted biomarkers that help understand the underlying mechanisms of male infertility; however, systematic information on the subject remains to be elucidated. The present study performed a comparative bioinformatics analysis to identify biomarkers associated with sperm quality, particular focusing on testis‑specific biomarkers. A stepwise screening approach identified 1,085 testis/epididymis‑specific genes and 3,406 teratospermia‑associated genes, resulting in 348 testis‑specific genes associated with aberrant sperm quality. These genes were functionally associated with the reproduction process. Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa. HSPA4L expression in sperm was revealed to be associated with sperm quality. The present study provided a novel insight into the understanding of sperm quality, and a potential method for the diagnosis and assessment of sperm quality in the event of male infertility.

No MeSH data available.


Related in: MedlinePlus

Cellular localization of heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 (PGK2) in human testis by immunohistochemistry. Scale bar, 20 µm.
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f3-mmr-14-02-1594: Cellular localization of heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 (PGK2) in human testis by immunohistochemistry. Scale bar, 20 µm.

Mentions: From the 348 key proteins, the chaperone protein, heat shock protein family A (Hsp70) member 4 like (HSP4AL) and metabolism protein, phosphoglycerate kinase 2 (PGK2), were selected for cellular localization investigation in the testis and epididymis tissue samples obtained from young and aged men. As presented in Fig. 3, HSPA4L was primarily expressed in round spermatids, whereas PGK2 was primarily expressed in spermatocytes and round spermatids. The proteins exhibited higher expression in the testis compared with the epididymis. In addition, the two proteins had lower expression levels in the testis samples from the aged men, compared with the younger men, thus suggesting there may be an age-related pattern of expression.


Screening targeted testis‑specific genes for molecular assessment of aberrant sperm quality.

Liu XX, Shen XF, Liu FJ - Mol Med Rep (2016)

Cellular localization of heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 (PGK2) in human testis by immunohistochemistry. Scale bar, 20 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940090&req=5

f3-mmr-14-02-1594: Cellular localization of heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 (PGK2) in human testis by immunohistochemistry. Scale bar, 20 µm.
Mentions: From the 348 key proteins, the chaperone protein, heat shock protein family A (Hsp70) member 4 like (HSP4AL) and metabolism protein, phosphoglycerate kinase 2 (PGK2), were selected for cellular localization investigation in the testis and epididymis tissue samples obtained from young and aged men. As presented in Fig. 3, HSPA4L was primarily expressed in round spermatids, whereas PGK2 was primarily expressed in spermatocytes and round spermatids. The proteins exhibited higher expression in the testis compared with the epididymis. In addition, the two proteins had lower expression levels in the testis samples from the aged men, compared with the younger men, thus suggesting there may be an age-related pattern of expression.

Bottom Line: Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility.These genes were functionally associated with the reproduction process.Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Central Laboratory, Yantai Yu Huang Ding Hospital, Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility. Genes and gene products associated with teratospermia may serve as targeted biomarkers that help understand the underlying mechanisms of male infertility; however, systematic information on the subject remains to be elucidated. The present study performed a comparative bioinformatics analysis to identify biomarkers associated with sperm quality, particular focusing on testis‑specific biomarkers. A stepwise screening approach identified 1,085 testis/epididymis‑specific genes and 3,406 teratospermia‑associated genes, resulting in 348 testis‑specific genes associated with aberrant sperm quality. These genes were functionally associated with the reproduction process. Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa. HSPA4L expression in sperm was revealed to be associated with sperm quality. The present study provided a novel insight into the understanding of sperm quality, and a potential method for the diagnosis and assessment of sperm quality in the event of male infertility.

No MeSH data available.


Related in: MedlinePlus