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Screening targeted testis‑specific genes for molecular assessment of aberrant sperm quality.

Liu XX, Shen XF, Liu FJ - Mol Med Rep (2016)

Bottom Line: Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility.These genes were functionally associated with the reproduction process.Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Central Laboratory, Yantai Yu Huang Ding Hospital, Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility. Genes and gene products associated with teratospermia may serve as targeted biomarkers that help understand the underlying mechanisms of male infertility; however, systematic information on the subject remains to be elucidated. The present study performed a comparative bioinformatics analysis to identify biomarkers associated with sperm quality, particular focusing on testis‑specific biomarkers. A stepwise screening approach identified 1,085 testis/epididymis‑specific genes and 3,406 teratospermia‑associated genes, resulting in 348 testis‑specific genes associated with aberrant sperm quality. These genes were functionally associated with the reproduction process. Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa. HSPA4L expression in sperm was revealed to be associated with sperm quality. The present study provided a novel insight into the understanding of sperm quality, and a potential method for the diagnosis and assessment of sperm quality in the event of male infertility.

No MeSH data available.


Related in: MedlinePlus

Broad functional analysis of proteins corresponding to (A) testis/epididymis-specific genes, (B) teratospermia-associated genes and (C) key targeted testis-specific teratospermia-associated genes.
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f2-mmr-14-02-1594: Broad functional analysis of proteins corresponding to (A) testis/epididymis-specific genes, (B) teratospermia-associated genes and (C) key targeted testis-specific teratospermia-associated genes.

Mentions: Chromosomal distribution analysis of the 1,085 identified genes highlighted that the genes were mainly expressed on chromosomes 17 (ratio=2.19) and 19 (ratio=2.67), whereas less were expressed on chromosomes Y (ratio=0.44) and 13 (ratio=0.55) (Table I). The gene ontology analysis demonstrated that proteins corresponding to these genes were primarily involved in functions of metabolism (22%), structure (16%), protease/protease inhibitor (16%), as well as the major functions of transcription (11%) and signal transduction (10%) (Fig. 2A). Over-representative analysis indicated that these proteins were significantly involved in the biological processes of cell cycle, mitosis and spermatogenesis (Table II).


Screening targeted testis‑specific genes for molecular assessment of aberrant sperm quality.

Liu XX, Shen XF, Liu FJ - Mol Med Rep (2016)

Broad functional analysis of proteins corresponding to (A) testis/epididymis-specific genes, (B) teratospermia-associated genes and (C) key targeted testis-specific teratospermia-associated genes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940090&req=5

f2-mmr-14-02-1594: Broad functional analysis of proteins corresponding to (A) testis/epididymis-specific genes, (B) teratospermia-associated genes and (C) key targeted testis-specific teratospermia-associated genes.
Mentions: Chromosomal distribution analysis of the 1,085 identified genes highlighted that the genes were mainly expressed on chromosomes 17 (ratio=2.19) and 19 (ratio=2.67), whereas less were expressed on chromosomes Y (ratio=0.44) and 13 (ratio=0.55) (Table I). The gene ontology analysis demonstrated that proteins corresponding to these genes were primarily involved in functions of metabolism (22%), structure (16%), protease/protease inhibitor (16%), as well as the major functions of transcription (11%) and signal transduction (10%) (Fig. 2A). Over-representative analysis indicated that these proteins were significantly involved in the biological processes of cell cycle, mitosis and spermatogenesis (Table II).

Bottom Line: Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility.These genes were functionally associated with the reproduction process.Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Central Laboratory, Yantai Yu Huang Ding Hospital, Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility. Genes and gene products associated with teratospermia may serve as targeted biomarkers that help understand the underlying mechanisms of male infertility; however, systematic information on the subject remains to be elucidated. The present study performed a comparative bioinformatics analysis to identify biomarkers associated with sperm quality, particular focusing on testis‑specific biomarkers. A stepwise screening approach identified 1,085 testis/epididymis‑specific genes and 3,406 teratospermia‑associated genes, resulting in 348 testis‑specific genes associated with aberrant sperm quality. These genes were functionally associated with the reproduction process. Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa. HSPA4L expression in sperm was revealed to be associated with sperm quality. The present study provided a novel insight into the understanding of sperm quality, and a potential method for the diagnosis and assessment of sperm quality in the event of male infertility.

No MeSH data available.


Related in: MedlinePlus