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Screening targeted testis‑specific genes for molecular assessment of aberrant sperm quality.

Liu XX, Shen XF, Liu FJ - Mol Med Rep (2016)

Bottom Line: Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility.These genes were functionally associated with the reproduction process.Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Central Laboratory, Yantai Yu Huang Ding Hospital, Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility. Genes and gene products associated with teratospermia may serve as targeted biomarkers that help understand the underlying mechanisms of male infertility; however, systematic information on the subject remains to be elucidated. The present study performed a comparative bioinformatics analysis to identify biomarkers associated with sperm quality, particular focusing on testis‑specific biomarkers. A stepwise screening approach identified 1,085 testis/epididymis‑specific genes and 3,406 teratospermia‑associated genes, resulting in 348 testis‑specific genes associated with aberrant sperm quality. These genes were functionally associated with the reproduction process. Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa. HSPA4L expression in sperm was revealed to be associated with sperm quality. The present study provided a novel insight into the understanding of sperm quality, and a potential method for the diagnosis and assessment of sperm quality in the event of male infertility.

No MeSH data available.


Related in: MedlinePlus

Flowchart of identification of key teratospermia-associated genes.
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f1-mmr-14-02-1594: Flowchart of identification of key teratospermia-associated genes.

Mentions: By comparing gene expression data sets from 11 normal tissues with that of the testis and epididymis, a total of 1,085 significantly expressed genes were identified in the testis and epididymis. From this total, 770 genes exhibited greater expression in the testis, and 97 genes in the epididymis. These 867 genes were used as background data for subsequent screening (Fig. 1).


Screening targeted testis‑specific genes for molecular assessment of aberrant sperm quality.

Liu XX, Shen XF, Liu FJ - Mol Med Rep (2016)

Flowchart of identification of key teratospermia-associated genes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940090&req=5

f1-mmr-14-02-1594: Flowchart of identification of key teratospermia-associated genes.
Mentions: By comparing gene expression data sets from 11 normal tissues with that of the testis and epididymis, a total of 1,085 significantly expressed genes were identified in the testis and epididymis. From this total, 770 genes exhibited greater expression in the testis, and 97 genes in the epididymis. These 867 genes were used as background data for subsequent screening (Fig. 1).

Bottom Line: Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility.These genes were functionally associated with the reproduction process.Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa.

View Article: PubMed Central - PubMed

Affiliation: Central Laboratory, Yantai Yu Huang Ding Hospital, Qingdao University, Yantai, Shandong 264000, P.R. China.

ABSTRACT
Teratospermia is a heterogeneous and complex disorder, which is closely associated with male fertility. Genes and gene products associated with teratospermia may serve as targeted biomarkers that help understand the underlying mechanisms of male infertility; however, systematic information on the subject remains to be elucidated. The present study performed a comparative bioinformatics analysis to identify biomarkers associated with sperm quality, particular focusing on testis‑specific biomarkers. A stepwise screening approach identified 1,085 testis/epididymis‑specific genes and 3,406 teratospermia‑associated genes, resulting in 348 testis‑specific genes associated with aberrant sperm quality. These genes were functionally associated with the reproduction process. Gene products corresponding to heat shock protein family A (Hsp70) member 4 like (HSPA4L) and phosphoglycerate kinase 2 were characterized at the cellular level in human testes and ejaculated spermatozoa. HSPA4L expression in sperm was revealed to be associated with sperm quality. The present study provided a novel insight into the understanding of sperm quality, and a potential method for the diagnosis and assessment of sperm quality in the event of male infertility.

No MeSH data available.


Related in: MedlinePlus