Limits...
Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

Gu N, You L, Shi C, Yang L, Pang L, Cui X, Ji C, Zheng W, Guo X - Mol Med Rep (2016)

Bottom Line: MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM.The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes.FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated With Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.

ABSTRACT
Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

No MeSH data available.


Related in: MedlinePlus

Effect of FFA on miR-199a-3p expression in human mature adipocytes. Reverse transcription-quantitative polymerase chain reaction was used to analyze miR-199a-3p expression in human mature adipocytes cultured with 1 mM FFAs at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). miR-199a-3p expression was normalized to (A) U6 or (B) miR-103 expression. Values are expressed as the mean ± standard deviation of three separate experiments. *P<0.05; ***P<0.001 vs. the 0 h group. FFA, free fatty acid; miR, microRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940088&req=5

f5-mmr-14-02-1180: Effect of FFA on miR-199a-3p expression in human mature adipocytes. Reverse transcription-quantitative polymerase chain reaction was used to analyze miR-199a-3p expression in human mature adipocytes cultured with 1 mM FFAs at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). miR-199a-3p expression was normalized to (A) U6 or (B) miR-103 expression. Values are expressed as the mean ± standard deviation of three separate experiments. *P<0.05; ***P<0.001 vs. the 0 h group. FFA, free fatty acid; miR, microRNA.

Mentions: To investigate the effect of FFAs on miR-199a-3p expression, mature human adipocytes following 15 days induced differentiation, were treated with 1 mM FFA at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). To confirm the regulation by FFAs, miR-103 and U6 expression were reference genes for normalization. RT-qPCR results indicated miR-199a-3p expression was significantly increased (P<0.001 when normalized to U6 and P<0.05 when normalized to miR-103 at 4 h) within 8 h, but decreased again over time (Fig. 5A and B).


Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

Gu N, You L, Shi C, Yang L, Pang L, Cui X, Ji C, Zheng W, Guo X - Mol Med Rep (2016)

Effect of FFA on miR-199a-3p expression in human mature adipocytes. Reverse transcription-quantitative polymerase chain reaction was used to analyze miR-199a-3p expression in human mature adipocytes cultured with 1 mM FFAs at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). miR-199a-3p expression was normalized to (A) U6 or (B) miR-103 expression. Values are expressed as the mean ± standard deviation of three separate experiments. *P<0.05; ***P<0.001 vs. the 0 h group. FFA, free fatty acid; miR, microRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940088&req=5

f5-mmr-14-02-1180: Effect of FFA on miR-199a-3p expression in human mature adipocytes. Reverse transcription-quantitative polymerase chain reaction was used to analyze miR-199a-3p expression in human mature adipocytes cultured with 1 mM FFAs at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). miR-199a-3p expression was normalized to (A) U6 or (B) miR-103 expression. Values are expressed as the mean ± standard deviation of three separate experiments. *P<0.05; ***P<0.001 vs. the 0 h group. FFA, free fatty acid; miR, microRNA.
Mentions: To investigate the effect of FFAs on miR-199a-3p expression, mature human adipocytes following 15 days induced differentiation, were treated with 1 mM FFA at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). To confirm the regulation by FFAs, miR-103 and U6 expression were reference genes for normalization. RT-qPCR results indicated miR-199a-3p expression was significantly increased (P<0.001 when normalized to U6 and P<0.05 when normalized to miR-103 at 4 h) within 8 h, but decreased again over time (Fig. 5A and B).

Bottom Line: MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM.The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes.FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated With Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.

ABSTRACT
Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

No MeSH data available.


Related in: MedlinePlus