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Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

Gu N, You L, Shi C, Yang L, Pang L, Cui X, Ji C, Zheng W, Guo X - Mol Med Rep (2016)

Bottom Line: MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM.The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes.FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated With Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.

ABSTRACT
Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

No MeSH data available.


Related in: MedlinePlus

Inflammatory cytokines, TNF-α and IL-6, increased miR-199a-3p expression in human mature adipocytes. Reverse transcription-quantitative polymerase chain reaction was used to analyze miR-199a-3p expression in human mature adipocytes cultured with (A and B) 10 ng/ml TNF-α or (C and D) 30 ng/ml IL-6 at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). U6 and miR-103 expression served as internal controls for normalization. Values are presented as the mean ± standard deviation of three separate experiments. *P<0.05; **P<0.01; ***P<0.001 vs. the 0 h group. miR, microRNA; TNF-α, tumor necrosis factor-α; IL, interleukin.
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f4-mmr-14-02-1180: Inflammatory cytokines, TNF-α and IL-6, increased miR-199a-3p expression in human mature adipocytes. Reverse transcription-quantitative polymerase chain reaction was used to analyze miR-199a-3p expression in human mature adipocytes cultured with (A and B) 10 ng/ml TNF-α or (C and D) 30 ng/ml IL-6 at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). U6 and miR-103 expression served as internal controls for normalization. Values are presented as the mean ± standard deviation of three separate experiments. *P<0.05; **P<0.01; ***P<0.001 vs. the 0 h group. miR, microRNA; TNF-α, tumor necrosis factor-α; IL, interleukin.

Mentions: To investigate the response of miR-199-3p to inflammatory cytokines, including TNF-α and IL-β, mature human adipocytes that had been differentiated for 15 days were treated with 10 ng/ml TNF-α and 30 ng/ml IL-β and harvested at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). miR-103 and U6 expression were used as reference genes for normalization in order to obtain consistent results. RT-qPCR results indicated that miR-199a-3p expression was gradually increased when mature human adipocytes were exposed to 10 ng/ml TNF-α, reaching a maximum at 8 h (P<0.01). The upregulation was sustained to 24 h (P<0.05; Fig. 4A and B). IL-β treatment exhibited a similar trend in mir-199a-3p expression (P<0.01 at 4 h, P<0.01 when normalized to U6 and P<0.001 when normalized to miR-103 at 8 h, P<0.001 when normalized to U6 and P<0.05 when normalized to miR-103; Fig. 4C and D).


Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

Gu N, You L, Shi C, Yang L, Pang L, Cui X, Ji C, Zheng W, Guo X - Mol Med Rep (2016)

Inflammatory cytokines, TNF-α and IL-6, increased miR-199a-3p expression in human mature adipocytes. Reverse transcription-quantitative polymerase chain reaction was used to analyze miR-199a-3p expression in human mature adipocytes cultured with (A and B) 10 ng/ml TNF-α or (C and D) 30 ng/ml IL-6 at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). U6 and miR-103 expression served as internal controls for normalization. Values are presented as the mean ± standard deviation of three separate experiments. *P<0.05; **P<0.01; ***P<0.001 vs. the 0 h group. miR, microRNA; TNF-α, tumor necrosis factor-α; IL, interleukin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940088&req=5

f4-mmr-14-02-1180: Inflammatory cytokines, TNF-α and IL-6, increased miR-199a-3p expression in human mature adipocytes. Reverse transcription-quantitative polymerase chain reaction was used to analyze miR-199a-3p expression in human mature adipocytes cultured with (A and B) 10 ng/ml TNF-α or (C and D) 30 ng/ml IL-6 at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). U6 and miR-103 expression served as internal controls for normalization. Values are presented as the mean ± standard deviation of three separate experiments. *P<0.05; **P<0.01; ***P<0.001 vs. the 0 h group. miR, microRNA; TNF-α, tumor necrosis factor-α; IL, interleukin.
Mentions: To investigate the response of miR-199-3p to inflammatory cytokines, including TNF-α and IL-β, mature human adipocytes that had been differentiated for 15 days were treated with 10 ng/ml TNF-α and 30 ng/ml IL-β and harvested at different time points (4, 8 and 24 h). Cells without treatment served as the control group (defined as 0 h). miR-103 and U6 expression were used as reference genes for normalization in order to obtain consistent results. RT-qPCR results indicated that miR-199a-3p expression was gradually increased when mature human adipocytes were exposed to 10 ng/ml TNF-α, reaching a maximum at 8 h (P<0.01). The upregulation was sustained to 24 h (P<0.05; Fig. 4A and B). IL-β treatment exhibited a similar trend in mir-199a-3p expression (P<0.01 at 4 h, P<0.01 when normalized to U6 and P<0.001 when normalized to miR-103 at 8 h, P<0.001 when normalized to U6 and P<0.05 when normalized to miR-103; Fig. 4C and D).

Bottom Line: MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM.The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes.FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated With Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.

ABSTRACT
Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

No MeSH data available.


Related in: MedlinePlus