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Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

Gu N, You L, Shi C, Yang L, Pang L, Cui X, Ji C, Zheng W, Guo X - Mol Med Rep (2016)

Bottom Line: MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM.The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes.FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated With Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.

ABSTRACT
Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

No MeSH data available.


Related in: MedlinePlus

Expression levels of miR-199a-3p are differentially regulated by leptin and resistin exposure. Following exposure of human mature adipocytes to (A and B) 100 ng/ml leptin or (C and D) 60 ng/ml resistin at different time points (4, 8 and 24 h), reverse transcription-quantitative polymerase chain reaction was adopted for miR-199a-3p expression. Cells without treatment were served as the control group (defined as 0 h). U6 and miR-103 expression served as internal controls for normalization. Values are presented as the mean ± standard deviation of three separate experiments. *P<0.05; **P<0.01 vs. the 0 h group. miR, microRNA.
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f3-mmr-14-02-1180: Expression levels of miR-199a-3p are differentially regulated by leptin and resistin exposure. Following exposure of human mature adipocytes to (A and B) 100 ng/ml leptin or (C and D) 60 ng/ml resistin at different time points (4, 8 and 24 h), reverse transcription-quantitative polymerase chain reaction was adopted for miR-199a-3p expression. Cells without treatment were served as the control group (defined as 0 h). U6 and miR-103 expression served as internal controls for normalization. Values are presented as the mean ± standard deviation of three separate experiments. *P<0.05; **P<0.01 vs. the 0 h group. miR, microRNA.

Mentions: To investigate the effect of leptin and resistin on miR-199a-3p, mature adipocytes, following 15 days induced differentiation, were treated with 100 ng/ml leptin and collected at 4, 8 and 24 h. Cells without treatment served as the control group (defined as 0 h). miR-103 and U6 served as reference genes for normalization in order to obtain consistent results. It was observed that leptin significantly stimulated miR-199a-3p expression at 4 h (P<0.01) but this effect decreased over time (Fig. 3A and B). By contrast, exposure of mature adipocytes to 60 ng/ml resistin resulted in a significant decrease in miR-199a-3p expression at 8 h (P<0.05 when normalized to U6, P<0.01 when normalized to miR-103; Fig. 3C and D).


Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

Gu N, You L, Shi C, Yang L, Pang L, Cui X, Ji C, Zheng W, Guo X - Mol Med Rep (2016)

Expression levels of miR-199a-3p are differentially regulated by leptin and resistin exposure. Following exposure of human mature adipocytes to (A and B) 100 ng/ml leptin or (C and D) 60 ng/ml resistin at different time points (4, 8 and 24 h), reverse transcription-quantitative polymerase chain reaction was adopted for miR-199a-3p expression. Cells without treatment were served as the control group (defined as 0 h). U6 and miR-103 expression served as internal controls for normalization. Values are presented as the mean ± standard deviation of three separate experiments. *P<0.05; **P<0.01 vs. the 0 h group. miR, microRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940088&req=5

f3-mmr-14-02-1180: Expression levels of miR-199a-3p are differentially regulated by leptin and resistin exposure. Following exposure of human mature adipocytes to (A and B) 100 ng/ml leptin or (C and D) 60 ng/ml resistin at different time points (4, 8 and 24 h), reverse transcription-quantitative polymerase chain reaction was adopted for miR-199a-3p expression. Cells without treatment were served as the control group (defined as 0 h). U6 and miR-103 expression served as internal controls for normalization. Values are presented as the mean ± standard deviation of three separate experiments. *P<0.05; **P<0.01 vs. the 0 h group. miR, microRNA.
Mentions: To investigate the effect of leptin and resistin on miR-199a-3p, mature adipocytes, following 15 days induced differentiation, were treated with 100 ng/ml leptin and collected at 4, 8 and 24 h. Cells without treatment served as the control group (defined as 0 h). miR-103 and U6 served as reference genes for normalization in order to obtain consistent results. It was observed that leptin significantly stimulated miR-199a-3p expression at 4 h (P<0.01) but this effect decreased over time (Fig. 3A and B). By contrast, exposure of mature adipocytes to 60 ng/ml resistin resulted in a significant decrease in miR-199a-3p expression at 8 h (P<0.05 when normalized to U6, P<0.01 when normalized to miR-103; Fig. 3C and D).

Bottom Line: MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM.The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes.FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated With Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.

ABSTRACT
Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

No MeSH data available.


Related in: MedlinePlus