Limits...
Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

Gu N, You L, Shi C, Yang L, Pang L, Cui X, Ji C, Zheng W, Guo X - Mol Med Rep (2016)

Bottom Line: MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM.The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes.FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated With Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.

ABSTRACT
Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

No MeSH data available.


Related in: MedlinePlus

Prior to and following differentiation of human pre-adipocytes. Representative micrograph of human pre-adipocytes on (A) day 0, and oil red O staining of lipid droplets accumulation in differentiated adipocytes on (B) day 4 and (C) day 15.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940088&req=5

f1-mmr-14-02-1180: Prior to and following differentiation of human pre-adipocytes. Representative micrograph of human pre-adipocytes on (A) day 0, and oil red O staining of lipid droplets accumulation in differentiated adipocytes on (B) day 4 and (C) day 15.

Mentions: For adipocyte differentiation, cells were grown to confluence as day 0 (Fig. 1A). Adipocytes differentiated for 4 and 15 days exhibited oil red O staining (Fig. 1B and C). After 15 days induction of differentiation, >80% of the adipocytes were observed with lipid droplet accumulation. miR-199a-3p expression between pre-adipocytes (day 0) and mature adipocytes (day 15) was evaluated by RT-qPCR. It was demonstrated the expression level of miR-199a-3p in mature adipocytes increased ~3.4 fold change compared with that in human pre-adipocytes (Fig. 2A; P<0.05).


Expression of miR-199a-3p in human adipocytes is regulated by free fatty acids and adipokines.

Gu N, You L, Shi C, Yang L, Pang L, Cui X, Ji C, Zheng W, Guo X - Mol Med Rep (2016)

Prior to and following differentiation of human pre-adipocytes. Representative micrograph of human pre-adipocytes on (A) day 0, and oil red O staining of lipid droplets accumulation in differentiated adipocytes on (B) day 4 and (C) day 15.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940088&req=5

f1-mmr-14-02-1180: Prior to and following differentiation of human pre-adipocytes. Representative micrograph of human pre-adipocytes on (A) day 0, and oil red O staining of lipid droplets accumulation in differentiated adipocytes on (B) day 4 and (C) day 15.
Mentions: For adipocyte differentiation, cells were grown to confluence as day 0 (Fig. 1A). Adipocytes differentiated for 4 and 15 days exhibited oil red O staining (Fig. 1B and C). After 15 days induction of differentiation, >80% of the adipocytes were observed with lipid droplet accumulation. miR-199a-3p expression between pre-adipocytes (day 0) and mature adipocytes (day 15) was evaluated by RT-qPCR. It was demonstrated the expression level of miR-199a-3p in mature adipocytes increased ~3.4 fold change compared with that in human pre-adipocytes (Fig. 2A; P<0.05).

Bottom Line: MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM.The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes.FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital Affiliated With Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China.

ABSTRACT
Obesity is associated with a notable risk for disease, including risk of cardiovascular disorders, type 2 diabetes mellitus (T2DM) and hypertension. Adipose tissue modulates the metabolism by releasing free fatty acids (FFAs) and adipokines, including leptin, resistin, tumor necrosis factor-α (TNF-α) and interleukin 6 (IL‑6). Altered secretion patterns of FFAs and adipokines have been demonstrated to result in obesity‑associated insulin resistance (IR) and inflammatory responses. MicroRNA-199a-3p (miR)-199a-3p expression is significantly induced in differentiated human adipose-derived mesenchymal stem cells and indicates the association with T2DM. However, the association between miR-199a-3p levels in adipocytes and obesity‑associated IR, as well as inflammatory responses remains to be elucidated. The present study observed an elevation of miR‑199a‑3p expression level in mature human adipocytes (visceral) compared with pre-adipocytes. In addition, miR‑199a‑3p expression was higher in visceral adipose deposits from obese subjects. FFA, TNF-α, IL‑6 and leptin significantly induced miR‑199a‑3p expression in mature human adipocytes, while resistin had the opposite effect. miR‑199a‑3p may represent a factor in the modulation of obesity‑associated IR and inflammatory responses.

No MeSH data available.


Related in: MedlinePlus