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Flowers of Camellia nitidissima cause growth inhibition, cell-cycle dysregulation and apoptosis in a human esophageal squamous cell carcinoma cell line.

Dai L, Li JL, Liang XQ, Li L, Feng Y, Liu HZ, Wei WE, Ning SF, Zhang LT - Mol Med Rep (2016)

Bottom Line: The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay.CNFE also caused dose‑ and time‑dependent apoptosis of these cells.Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle.

View Article: PubMed Central - PubMed

Affiliation: Research Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

ABSTRACT
The present study aimed to investigate the chemopreventive effect of Camellia nitidissima flowers water extract (CNFE) on the Eca109 human esophageal squamous cell carcinoma (ESCC) cell line. The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay. The effects of CNFE on apoptosis and cell cycle arrest were investigated by flow cytometry. CNFE inhibited cell growth in both a dose‑ and time‑dependent manner in Eca109 cells. CNFE also caused dose‑ and time‑dependent apoptosis of these cells. Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle. The data demonstrated that CNFE serves antiproliferative effects against human ESCC Eca109 cells by inducing apoptosis and interrupting the cell cycle. These results suggested that CNFE has the potential to be a chemoprotective agent for ESCC.

No MeSH data available.


Related in: MedlinePlus

Effect of CNFE on cell-cycle perturbation at different time points. (A) Eca109 cells were treated with vehicle or CNFE (200 µg/ml) for 12, 36 and 48 h, labeled with PI and analyzed by flow cytometry. The data shown are from a representative experiment repeated three times with similar results. (B) The histograms were obtained by PI staining. CNFE, Camellia nitidissima flowers water extract; PI, propidium iodide.
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f5-mmr-14-02-1117: Effect of CNFE on cell-cycle perturbation at different time points. (A) Eca109 cells were treated with vehicle or CNFE (200 µg/ml) for 12, 36 and 48 h, labeled with PI and analyzed by flow cytometry. The data shown are from a representative experiment repeated three times with similar results. (B) The histograms were obtained by PI staining. CNFE, Camellia nitidissima flowers water extract; PI, propidium iodide.

Mentions: To study whether CNFE can interrupt the cell cycle, flow cytometric analysis with PI staining was used to analyze the distribution of the cell cycle. After treatment with CNFE at 200 µg/ml for 24 h, the number of cells in G0/G1 phase was 61.67%, which was significantly more compared with the 51.66% observed in control cells (P<0.001; Fig. 4). The S phase cell population and G2/M phase cell population were significantly reduced to 34.30 and 4.04%, compared with the 36.44 and 11.91% observed in control cells (P<0.001; Fig. 4), respectively. The G0/G1 arrest effect of CNFE on Eca109 cells in G0/G1 phase was dose-dependent (P<0.001; Fig. 4). However, this effect appeared not to be time-dependent. Even when the incubation time was extended to 48 h, the cell cycle distribution revealed no significant change when compared with that at 12 or 36 h (Fig. 5).


Flowers of Camellia nitidissima cause growth inhibition, cell-cycle dysregulation and apoptosis in a human esophageal squamous cell carcinoma cell line.

Dai L, Li JL, Liang XQ, Li L, Feng Y, Liu HZ, Wei WE, Ning SF, Zhang LT - Mol Med Rep (2016)

Effect of CNFE on cell-cycle perturbation at different time points. (A) Eca109 cells were treated with vehicle or CNFE (200 µg/ml) for 12, 36 and 48 h, labeled with PI and analyzed by flow cytometry. The data shown are from a representative experiment repeated three times with similar results. (B) The histograms were obtained by PI staining. CNFE, Camellia nitidissima flowers water extract; PI, propidium iodide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940084&req=5

f5-mmr-14-02-1117: Effect of CNFE on cell-cycle perturbation at different time points. (A) Eca109 cells were treated with vehicle or CNFE (200 µg/ml) for 12, 36 and 48 h, labeled with PI and analyzed by flow cytometry. The data shown are from a representative experiment repeated three times with similar results. (B) The histograms were obtained by PI staining. CNFE, Camellia nitidissima flowers water extract; PI, propidium iodide.
Mentions: To study whether CNFE can interrupt the cell cycle, flow cytometric analysis with PI staining was used to analyze the distribution of the cell cycle. After treatment with CNFE at 200 µg/ml for 24 h, the number of cells in G0/G1 phase was 61.67%, which was significantly more compared with the 51.66% observed in control cells (P<0.001; Fig. 4). The S phase cell population and G2/M phase cell population were significantly reduced to 34.30 and 4.04%, compared with the 36.44 and 11.91% observed in control cells (P<0.001; Fig. 4), respectively. The G0/G1 arrest effect of CNFE on Eca109 cells in G0/G1 phase was dose-dependent (P<0.001; Fig. 4). However, this effect appeared not to be time-dependent. Even when the incubation time was extended to 48 h, the cell cycle distribution revealed no significant change when compared with that at 12 or 36 h (Fig. 5).

Bottom Line: The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay.CNFE also caused dose‑ and time‑dependent apoptosis of these cells.Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle.

View Article: PubMed Central - PubMed

Affiliation: Research Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

ABSTRACT
The present study aimed to investigate the chemopreventive effect of Camellia nitidissima flowers water extract (CNFE) on the Eca109 human esophageal squamous cell carcinoma (ESCC) cell line. The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay. The effects of CNFE on apoptosis and cell cycle arrest were investigated by flow cytometry. CNFE inhibited cell growth in both a dose‑ and time‑dependent manner in Eca109 cells. CNFE also caused dose‑ and time‑dependent apoptosis of these cells. Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle. The data demonstrated that CNFE serves antiproliferative effects against human ESCC Eca109 cells by inducing apoptosis and interrupting the cell cycle. These results suggested that CNFE has the potential to be a chemoprotective agent for ESCC.

No MeSH data available.


Related in: MedlinePlus