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Flowers of Camellia nitidissima cause growth inhibition, cell-cycle dysregulation and apoptosis in a human esophageal squamous cell carcinoma cell line.

Dai L, Li JL, Liang XQ, Li L, Feng Y, Liu HZ, Wei WE, Ning SF, Zhang LT - Mol Med Rep (2016)

Bottom Line: The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay.CNFE also caused dose‑ and time‑dependent apoptosis of these cells.Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle.

View Article: PubMed Central - PubMed

Affiliation: Research Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

ABSTRACT
The present study aimed to investigate the chemopreventive effect of Camellia nitidissima flowers water extract (CNFE) on the Eca109 human esophageal squamous cell carcinoma (ESCC) cell line. The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay. The effects of CNFE on apoptosis and cell cycle arrest were investigated by flow cytometry. CNFE inhibited cell growth in both a dose‑ and time‑dependent manner in Eca109 cells. CNFE also caused dose‑ and time‑dependent apoptosis of these cells. Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle. The data demonstrated that CNFE serves antiproliferative effects against human ESCC Eca109 cells by inducing apoptosis and interrupting the cell cycle. These results suggested that CNFE has the potential to be a chemoprotective agent for ESCC.

No MeSH data available.


Related in: MedlinePlus

Apoptosis of Eca109 cells induced by CNFE at different time points. Eca109 cells were treated with vehicle or CNFE (300 µg/ml) for 24, 48 and 72 h, labeled with fluorescein isothiocyanate-Annexin V and PI, and analyzed by flow cytometry. Representative data are from three independent experiments. **P<0.01 vs. the control. CNFE, Camellia nitidissima flowers water extract; PI, propidium iodide; h, hours.
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f3-mmr-14-02-1117: Apoptosis of Eca109 cells induced by CNFE at different time points. Eca109 cells were treated with vehicle or CNFE (300 µg/ml) for 24, 48 and 72 h, labeled with fluorescein isothiocyanate-Annexin V and PI, and analyzed by flow cytometry. Representative data are from three independent experiments. **P<0.01 vs. the control. CNFE, Camellia nitidissima flowers water extract; PI, propidium iodide; h, hours.

Mentions: The apoptosis of Eca109 cells was further analyzed by flow cytometry using FITC-Annexin V and PI (Fig. 2). In control cells, the percentage of early apoptotic cells was 3.01% and the percentage of late apoptotic/dead cells was 5.91% (Fig. 2). Following incubation with 100 µg/ml CNFE for 48 h, the percentages of early apoptotic cells and late apoptotic cells increased to 14.16 and 7.27%, respectively (P<0.01; Fig. 2). The apoptosis of Eca109 cells after treatment with 100–500 µg/ml CNFE for 48 h was significantly increased in a dose-dependent manner (Fig. 2). The percentage of apoptotic cells (early and late apoptosis in total) gradually increased as the incubating time was extended. The percentage of apoptotic Eca109 cells following treatment with 300 µg/ml CNFE for 24, 48 and 72 h were 16.55, 25.14 and 30.72% respectively, each significantly increased compared with that of the control cells (9.09, 8.92 and 15.07%, respectively; P<0.001; Fig. 3).


Flowers of Camellia nitidissima cause growth inhibition, cell-cycle dysregulation and apoptosis in a human esophageal squamous cell carcinoma cell line.

Dai L, Li JL, Liang XQ, Li L, Feng Y, Liu HZ, Wei WE, Ning SF, Zhang LT - Mol Med Rep (2016)

Apoptosis of Eca109 cells induced by CNFE at different time points. Eca109 cells were treated with vehicle or CNFE (300 µg/ml) for 24, 48 and 72 h, labeled with fluorescein isothiocyanate-Annexin V and PI, and analyzed by flow cytometry. Representative data are from three independent experiments. **P<0.01 vs. the control. CNFE, Camellia nitidissima flowers water extract; PI, propidium iodide; h, hours.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940084&req=5

f3-mmr-14-02-1117: Apoptosis of Eca109 cells induced by CNFE at different time points. Eca109 cells were treated with vehicle or CNFE (300 µg/ml) for 24, 48 and 72 h, labeled with fluorescein isothiocyanate-Annexin V and PI, and analyzed by flow cytometry. Representative data are from three independent experiments. **P<0.01 vs. the control. CNFE, Camellia nitidissima flowers water extract; PI, propidium iodide; h, hours.
Mentions: The apoptosis of Eca109 cells was further analyzed by flow cytometry using FITC-Annexin V and PI (Fig. 2). In control cells, the percentage of early apoptotic cells was 3.01% and the percentage of late apoptotic/dead cells was 5.91% (Fig. 2). Following incubation with 100 µg/ml CNFE for 48 h, the percentages of early apoptotic cells and late apoptotic cells increased to 14.16 and 7.27%, respectively (P<0.01; Fig. 2). The apoptosis of Eca109 cells after treatment with 100–500 µg/ml CNFE for 48 h was significantly increased in a dose-dependent manner (Fig. 2). The percentage of apoptotic cells (early and late apoptosis in total) gradually increased as the incubating time was extended. The percentage of apoptotic Eca109 cells following treatment with 300 µg/ml CNFE for 24, 48 and 72 h were 16.55, 25.14 and 30.72% respectively, each significantly increased compared with that of the control cells (9.09, 8.92 and 15.07%, respectively; P<0.001; Fig. 3).

Bottom Line: The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay.CNFE also caused dose‑ and time‑dependent apoptosis of these cells.Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle.

View Article: PubMed Central - PubMed

Affiliation: Research Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

ABSTRACT
The present study aimed to investigate the chemopreventive effect of Camellia nitidissima flowers water extract (CNFE) on the Eca109 human esophageal squamous cell carcinoma (ESCC) cell line. The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay. The effects of CNFE on apoptosis and cell cycle arrest were investigated by flow cytometry. CNFE inhibited cell growth in both a dose‑ and time‑dependent manner in Eca109 cells. CNFE also caused dose‑ and time‑dependent apoptosis of these cells. Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle. The data demonstrated that CNFE serves antiproliferative effects against human ESCC Eca109 cells by inducing apoptosis and interrupting the cell cycle. These results suggested that CNFE has the potential to be a chemoprotective agent for ESCC.

No MeSH data available.


Related in: MedlinePlus