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Flowers of Camellia nitidissima cause growth inhibition, cell-cycle dysregulation and apoptosis in a human esophageal squamous cell carcinoma cell line.

Dai L, Li JL, Liang XQ, Li L, Feng Y, Liu HZ, Wei WE, Ning SF, Zhang LT - Mol Med Rep (2016)

Bottom Line: The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay.CNFE also caused dose‑ and time‑dependent apoptosis of these cells.Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle.

View Article: PubMed Central - PubMed

Affiliation: Research Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

ABSTRACT
The present study aimed to investigate the chemopreventive effect of Camellia nitidissima flowers water extract (CNFE) on the Eca109 human esophageal squamous cell carcinoma (ESCC) cell line. The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay. The effects of CNFE on apoptosis and cell cycle arrest were investigated by flow cytometry. CNFE inhibited cell growth in both a dose‑ and time‑dependent manner in Eca109 cells. CNFE also caused dose‑ and time‑dependent apoptosis of these cells. Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle. The data demonstrated that CNFE serves antiproliferative effects against human ESCC Eca109 cells by inducing apoptosis and interrupting the cell cycle. These results suggested that CNFE has the potential to be a chemoprotective agent for ESCC.

No MeSH data available.


Related in: MedlinePlus

Viability of Eca109 cells following treatment with CNFE. Eca109 cells were exposed to different concentrations of CNFE for up to 72 h. The percentage of dead cells was determined using a trypan blue exclusion assay. Each experiment was performed independently three times. The data are presented as the mean ± standard deviation (**P<0.001, compared with the control at 24, 48 and 72 h. CNFE, Camellia nitidissima flowers water extract; h, hours.
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f1-mmr-14-02-1117: Viability of Eca109 cells following treatment with CNFE. Eca109 cells were exposed to different concentrations of CNFE for up to 72 h. The percentage of dead cells was determined using a trypan blue exclusion assay. Each experiment was performed independently three times. The data are presented as the mean ± standard deviation (**P<0.001, compared with the control at 24, 48 and 72 h. CNFE, Camellia nitidissima flowers water extract; h, hours.

Mentions: Firstly, the present study investigated the effects of CNFE on the viability of Eca109 cells by trypan blue exclusion assay (Fig. 1). As shown in Fig. 1, the percentage of dead cells increased both in a time- and dose-dependent manner. Compared with the non-treated control, the percentage of dead cells when Eca109 cells were treated with 300 µg/ml of CNFE for 24 h was significantly increased (34.43 vs. 6.43%). The cell death of Eca109 cells increased gradually when treated with CNFE at 300, 400 and 500 µg/ml for 24 h (P<0.001, compared with the control; Fig. 1). The percentage of dead cells when Eca109 cells were treated with CNFE (300, 400 or 500 µg/ml) for 24 h was 34.42, 37.30 and 39.43%, respectively. Treatment with 200 µg/ml CNFE for 48 or 72 h significantly reduced the cell viability (P<0.001, compared with control; Fig. 1). The antiproliferative effect of CNFE was also time-dependent. The IC50 of CNFE on Eca109 cells, calculated accordingly at 24, 48 and 72 h, was 513.64, 326.88 and 217.31 µg/ml, respectively (Fig. 1).


Flowers of Camellia nitidissima cause growth inhibition, cell-cycle dysregulation and apoptosis in a human esophageal squamous cell carcinoma cell line.

Dai L, Li JL, Liang XQ, Li L, Feng Y, Liu HZ, Wei WE, Ning SF, Zhang LT - Mol Med Rep (2016)

Viability of Eca109 cells following treatment with CNFE. Eca109 cells were exposed to different concentrations of CNFE for up to 72 h. The percentage of dead cells was determined using a trypan blue exclusion assay. Each experiment was performed independently three times. The data are presented as the mean ± standard deviation (**P<0.001, compared with the control at 24, 48 and 72 h. CNFE, Camellia nitidissima flowers water extract; h, hours.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940084&req=5

f1-mmr-14-02-1117: Viability of Eca109 cells following treatment with CNFE. Eca109 cells were exposed to different concentrations of CNFE for up to 72 h. The percentage of dead cells was determined using a trypan blue exclusion assay. Each experiment was performed independently three times. The data are presented as the mean ± standard deviation (**P<0.001, compared with the control at 24, 48 and 72 h. CNFE, Camellia nitidissima flowers water extract; h, hours.
Mentions: Firstly, the present study investigated the effects of CNFE on the viability of Eca109 cells by trypan blue exclusion assay (Fig. 1). As shown in Fig. 1, the percentage of dead cells increased both in a time- and dose-dependent manner. Compared with the non-treated control, the percentage of dead cells when Eca109 cells were treated with 300 µg/ml of CNFE for 24 h was significantly increased (34.43 vs. 6.43%). The cell death of Eca109 cells increased gradually when treated with CNFE at 300, 400 and 500 µg/ml for 24 h (P<0.001, compared with the control; Fig. 1). The percentage of dead cells when Eca109 cells were treated with CNFE (300, 400 or 500 µg/ml) for 24 h was 34.42, 37.30 and 39.43%, respectively. Treatment with 200 µg/ml CNFE for 48 or 72 h significantly reduced the cell viability (P<0.001, compared with control; Fig. 1). The antiproliferative effect of CNFE was also time-dependent. The IC50 of CNFE on Eca109 cells, calculated accordingly at 24, 48 and 72 h, was 513.64, 326.88 and 217.31 µg/ml, respectively (Fig. 1).

Bottom Line: The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay.CNFE also caused dose‑ and time‑dependent apoptosis of these cells.Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle.

View Article: PubMed Central - PubMed

Affiliation: Research Department, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.

ABSTRACT
The present study aimed to investigate the chemopreventive effect of Camellia nitidissima flowers water extract (CNFE) on the Eca109 human esophageal squamous cell carcinoma (ESCC) cell line. The antiproliferative effect on Eca109 cells was determined using the trypan blue exclusion assay. The effects of CNFE on apoptosis and cell cycle arrest were investigated by flow cytometry. CNFE inhibited cell growth in both a dose‑ and time‑dependent manner in Eca109 cells. CNFE also caused dose‑ and time‑dependent apoptosis of these cells. Treatment of cells with CNFE resulted in dose‑dependent G0/G1 phase arrest of the cell cycle. The data demonstrated that CNFE serves antiproliferative effects against human ESCC Eca109 cells by inducing apoptosis and interrupting the cell cycle. These results suggested that CNFE has the potential to be a chemoprotective agent for ESCC.

No MeSH data available.


Related in: MedlinePlus