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Histone acetylation is involved in TCDD‑induced cleft palate formation in fetal mice.

Yuan X, Qiu L, Pu Y, Liu C, Zhang X, Wang C, Pu W, Fu Y - Mol Med Rep (2016)

Bottom Line: On GD 13.5 and GD 14.5, TGF‑β3 mRNA expression, HAT activity and acetylated H3 levels were significantly increased in the TCDD group compared with the control.Methylated bands were not observed in the TCDD or control groups.In conclusion, at the critical period of palate fusion (GD 13.5‑14.5), TCDD significantly increased TGF‑β3 gene expression, HAT activity and H3 acetylation.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, P.R. China.

ABSTRACT
The aim of the present was to evaluate the effects of DNA methylation and histone acetylation on 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (TCDD)‑induced cleft palate in fetal mice. Pregnant mice (n=10) were randomly divided into two groups: i) TCDD group, mice were treated with 28 µg/kg TCDD on gestation day (GD) 10 by oral gavage; ii) control group, mice were treated with an equal volume of corn oil. On GD 16.5, the fetal mice were evaluated for the presence of a cleft palate. An additional 36 pregnant mice were divided into the control and TCDD groups, and palate samples were collected on GD 13.5, GD 14.5 and GD 15.5, respectively. Transforming growth factor‑β3 (TGF‑β3) mRNA expression, TGF‑β3 promoter methylation, histone acetyltransferase (HAT) activity and histone H3 (H3) acetylation in the palates were evaluated in the two groups. The incidence of a cleft palate in the TCDD group was 93.55%, and no cases of cleft palate were identified in the control group. On GD 13.5 and GD 14.5, TGF‑β3 mRNA expression, HAT activity and acetylated H3 levels were significantly increased in the TCDD group compared with the control. Methylated bands were not observed in the TCDD or control groups. In conclusion, at the critical period of palate fusion (GD 13.5‑14.5), TCDD significantly increased TGF‑β3 gene expression, HAT activity and H3 acetylation. Therefore, histone acetylation may be involved in TCDD‑induced cleft palate formation in fetal mice.

No MeSH data available.


Related in: MedlinePlus

Detection of Ac-H3 in the TCDD and control groups by western blotting. (A) Representative blot. (B) Statistical analysis of the acetylated histone H3 level. Data are presented as the mean ± standard deviation. *P<0.01 and #P<0.05 vs. the control group. N, control group, T, TCDD group. Ac-H3, acetylated histone 3; GD, gestation day; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.
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f6-mmr-14-02-1139: Detection of Ac-H3 in the TCDD and control groups by western blotting. (A) Representative blot. (B) Statistical analysis of the acetylated histone H3 level. Data are presented as the mean ± standard deviation. *P<0.01 and #P<0.05 vs. the control group. N, control group, T, TCDD group. Ac-H3, acetylated histone 3; GD, gestation day; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.

Mentions: The level of H3 acetylation in the TCDD group was significantly increased on GD 13.5 and GD 14.5 compared with the control group (P<0.01 and P<0.05, respectively; Fig. 6). On GD 15.5, it was significantly reduced (P<0.05; Fig. 6). H3 acetylation level in the control group was increased from GD 13.5 to GD 15.5; however, in the TCDD group, it was not significantly affected from GD 13.5 to GD 15.5 (Fig. 6).


Histone acetylation is involved in TCDD‑induced cleft palate formation in fetal mice.

Yuan X, Qiu L, Pu Y, Liu C, Zhang X, Wang C, Pu W, Fu Y - Mol Med Rep (2016)

Detection of Ac-H3 in the TCDD and control groups by western blotting. (A) Representative blot. (B) Statistical analysis of the acetylated histone H3 level. Data are presented as the mean ± standard deviation. *P<0.01 and #P<0.05 vs. the control group. N, control group, T, TCDD group. Ac-H3, acetylated histone 3; GD, gestation day; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940082&req=5

f6-mmr-14-02-1139: Detection of Ac-H3 in the TCDD and control groups by western blotting. (A) Representative blot. (B) Statistical analysis of the acetylated histone H3 level. Data are presented as the mean ± standard deviation. *P<0.01 and #P<0.05 vs. the control group. N, control group, T, TCDD group. Ac-H3, acetylated histone 3; GD, gestation day; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Mentions: The level of H3 acetylation in the TCDD group was significantly increased on GD 13.5 and GD 14.5 compared with the control group (P<0.01 and P<0.05, respectively; Fig. 6). On GD 15.5, it was significantly reduced (P<0.05; Fig. 6). H3 acetylation level in the control group was increased from GD 13.5 to GD 15.5; however, in the TCDD group, it was not significantly affected from GD 13.5 to GD 15.5 (Fig. 6).

Bottom Line: On GD 13.5 and GD 14.5, TGF‑β3 mRNA expression, HAT activity and acetylated H3 levels were significantly increased in the TCDD group compared with the control.Methylated bands were not observed in the TCDD or control groups.In conclusion, at the critical period of palate fusion (GD 13.5‑14.5), TCDD significantly increased TGF‑β3 gene expression, HAT activity and H3 acetylation.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, P.R. China.

ABSTRACT
The aim of the present was to evaluate the effects of DNA methylation and histone acetylation on 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (TCDD)‑induced cleft palate in fetal mice. Pregnant mice (n=10) were randomly divided into two groups: i) TCDD group, mice were treated with 28 µg/kg TCDD on gestation day (GD) 10 by oral gavage; ii) control group, mice were treated with an equal volume of corn oil. On GD 16.5, the fetal mice were evaluated for the presence of a cleft palate. An additional 36 pregnant mice were divided into the control and TCDD groups, and palate samples were collected on GD 13.5, GD 14.5 and GD 15.5, respectively. Transforming growth factor‑β3 (TGF‑β3) mRNA expression, TGF‑β3 promoter methylation, histone acetyltransferase (HAT) activity and histone H3 (H3) acetylation in the palates were evaluated in the two groups. The incidence of a cleft palate in the TCDD group was 93.55%, and no cases of cleft palate were identified in the control group. On GD 13.5 and GD 14.5, TGF‑β3 mRNA expression, HAT activity and acetylated H3 levels were significantly increased in the TCDD group compared with the control. Methylated bands were not observed in the TCDD or control groups. In conclusion, at the critical period of palate fusion (GD 13.5‑14.5), TCDD significantly increased TGF‑β3 gene expression, HAT activity and H3 acetylation. Therefore, histone acetylation may be involved in TCDD‑induced cleft palate formation in fetal mice.

No MeSH data available.


Related in: MedlinePlus