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Histone acetylation is involved in TCDD‑induced cleft palate formation in fetal mice.

Yuan X, Qiu L, Pu Y, Liu C, Zhang X, Wang C, Pu W, Fu Y - Mol Med Rep (2016)

Bottom Line: On GD 13.5 and GD 14.5, TGF‑β3 mRNA expression, HAT activity and acetylated H3 levels were significantly increased in the TCDD group compared with the control.Methylated bands were not observed in the TCDD or control groups.In conclusion, at the critical period of palate fusion (GD 13.5‑14.5), TCDD significantly increased TGF‑β3 gene expression, HAT activity and H3 acetylation.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, P.R. China.

ABSTRACT
The aim of the present was to evaluate the effects of DNA methylation and histone acetylation on 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (TCDD)‑induced cleft palate in fetal mice. Pregnant mice (n=10) were randomly divided into two groups: i) TCDD group, mice were treated with 28 µg/kg TCDD on gestation day (GD) 10 by oral gavage; ii) control group, mice were treated with an equal volume of corn oil. On GD 16.5, the fetal mice were evaluated for the presence of a cleft palate. An additional 36 pregnant mice were divided into the control and TCDD groups, and palate samples were collected on GD 13.5, GD 14.5 and GD 15.5, respectively. Transforming growth factor‑β3 (TGF‑β3) mRNA expression, TGF‑β3 promoter methylation, histone acetyltransferase (HAT) activity and histone H3 (H3) acetylation in the palates were evaluated in the two groups. The incidence of a cleft palate in the TCDD group was 93.55%, and no cases of cleft palate were identified in the control group. On GD 13.5 and GD 14.5, TGF‑β3 mRNA expression, HAT activity and acetylated H3 levels were significantly increased in the TCDD group compared with the control. Methylated bands were not observed in the TCDD or control groups. In conclusion, at the critical period of palate fusion (GD 13.5‑14.5), TCDD significantly increased TGF‑β3 gene expression, HAT activity and H3 acetylation. Therefore, histone acetylation may be involved in TCDD‑induced cleft palate formation in fetal mice.

No MeSH data available.


Related in: MedlinePlus

Histology of the palate on GD 16.5. (A) Control group and (B) TCDD group. Stained with hematoxylin and eosin. Magnification, ×40. GD, gestation day; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.
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f2-mmr-14-02-1139: Histology of the palate on GD 16.5. (A) Control group and (B) TCDD group. Stained with hematoxylin and eosin. Magnification, ×40. GD, gestation day; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.

Mentions: The appearance, feeding, drinking, and activity of the mice were not affected by TCDD treatment. There was no significant difference in the weight gain of pregnant mice, body weights of live fetal mice, or the numbers of live fetuses per litter in the TCDD group compared with the control group (P>0.05, Table II). No cleft palates were observed in fetal mice in the control group, while 93.55% of fetal mice in the TCDD group had a cleft palate. The incidence of a cleft palate was significantly different between the two groups (P<0.01, Table II). Additionally, on GD 16.5, the palatine process fused to the opposite side and formed a complete palate in the control group, whereas the palatine process in the TCDD group was not complete and a cleft was observed in the middle (Fig. 2).


Histone acetylation is involved in TCDD‑induced cleft palate formation in fetal mice.

Yuan X, Qiu L, Pu Y, Liu C, Zhang X, Wang C, Pu W, Fu Y - Mol Med Rep (2016)

Histology of the palate on GD 16.5. (A) Control group and (B) TCDD group. Stained with hematoxylin and eosin. Magnification, ×40. GD, gestation day; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940082&req=5

f2-mmr-14-02-1139: Histology of the palate on GD 16.5. (A) Control group and (B) TCDD group. Stained with hematoxylin and eosin. Magnification, ×40. GD, gestation day; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.
Mentions: The appearance, feeding, drinking, and activity of the mice were not affected by TCDD treatment. There was no significant difference in the weight gain of pregnant mice, body weights of live fetal mice, or the numbers of live fetuses per litter in the TCDD group compared with the control group (P>0.05, Table II). No cleft palates were observed in fetal mice in the control group, while 93.55% of fetal mice in the TCDD group had a cleft palate. The incidence of a cleft palate was significantly different between the two groups (P<0.01, Table II). Additionally, on GD 16.5, the palatine process fused to the opposite side and formed a complete palate in the control group, whereas the palatine process in the TCDD group was not complete and a cleft was observed in the middle (Fig. 2).

Bottom Line: On GD 13.5 and GD 14.5, TGF‑β3 mRNA expression, HAT activity and acetylated H3 levels were significantly increased in the TCDD group compared with the control.Methylated bands were not observed in the TCDD or control groups.In conclusion, at the critical period of palate fusion (GD 13.5‑14.5), TCDD significantly increased TGF‑β3 gene expression, HAT activity and H3 acetylation.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing 400014, P.R. China.

ABSTRACT
The aim of the present was to evaluate the effects of DNA methylation and histone acetylation on 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin (TCDD)‑induced cleft palate in fetal mice. Pregnant mice (n=10) were randomly divided into two groups: i) TCDD group, mice were treated with 28 µg/kg TCDD on gestation day (GD) 10 by oral gavage; ii) control group, mice were treated with an equal volume of corn oil. On GD 16.5, the fetal mice were evaluated for the presence of a cleft palate. An additional 36 pregnant mice were divided into the control and TCDD groups, and palate samples were collected on GD 13.5, GD 14.5 and GD 15.5, respectively. Transforming growth factor‑β3 (TGF‑β3) mRNA expression, TGF‑β3 promoter methylation, histone acetyltransferase (HAT) activity and histone H3 (H3) acetylation in the palates were evaluated in the two groups. The incidence of a cleft palate in the TCDD group was 93.55%, and no cases of cleft palate were identified in the control group. On GD 13.5 and GD 14.5, TGF‑β3 mRNA expression, HAT activity and acetylated H3 levels were significantly increased in the TCDD group compared with the control. Methylated bands were not observed in the TCDD or control groups. In conclusion, at the critical period of palate fusion (GD 13.5‑14.5), TCDD significantly increased TGF‑β3 gene expression, HAT activity and H3 acetylation. Therefore, histone acetylation may be involved in TCDD‑induced cleft palate formation in fetal mice.

No MeSH data available.


Related in: MedlinePlus