Limits...
ATunable Scaffold of Microtubular Graphite for 3D Cell Growth

View Article: PubMed Central - PubMed

ABSTRACT

Aerographite(AG) is a novel carbon-based material that exists as a self-supportive3D network of interconnected hollow microtubules. It can be synthesizedin a variety of architectures tailored by the growth conditions. Thisflexibility in creating structures presents interesting bioengineeringpossibilities such as the generation of an artificial extracellularmatrix. Here we have explored the feasibility and potential of AGas a scaffold for 3D cell growth employing cyclic RGD (cRGD) peptidescoupled to poly(ethylene glycol) (PEG) conjugated phospholipids forsurface functionalization to promote specific adhesion of fibroblastcells. Successful growth and invasion of the bulk material was followedover a period of 4 days.

No MeSH data available.


PEG-lipid functionalized AG was subjected tosupercritical point drying followed by deposition of a thin layerof gold. (A–C) Gradual zoom-in reveals the adsorbed PEG-lipidmolecules at high magnification. (D) A 4:1 mixture of amine terminated(top) and cyclic RGD peptide (cRGD) functionalized PEG-lipids (bottom)was used to promote cell attachment by integrin-mediated binding tocRGD. (E) Gold-coated pristine AG exhibits a smooth surface.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940076&req=5

fig2: PEG-lipid functionalized AG was subjected tosupercritical point drying followed by deposition of a thin layerof gold. (A–C) Gradual zoom-in reveals the adsorbed PEG-lipidmolecules at high magnification. (D) A 4:1 mixture of amine terminated(top) and cyclic RGD peptide (cRGD) functionalized PEG-lipids (bottom)was used to promote cell attachment by integrin-mediated binding tocRGD. (E) Gold-coated pristine AG exhibits a smooth surface.

Mentions: Scanning electron microscopy (SEM) was performed to verify adsorptionof PEG-lipids on the outer surface of the filaments (Figure 2). AG samples were dehydratedby super critical point drying (CPD) to avoid the destructive effectof surface tension on the network during evaporation of the liquidand a thin layer of gold was applied to improve visualization of biomoleculeson the graphitic filaments. The PEG-lipids became visible at highmagnification as bright dots (Figure 2B, C), and were found to decorate the surface as adense and homogeneous monolayer of individual molecules. Control experimentswith gold-coated pristine AG confirmed that the observed nanostructureswere not artifacts of the coating procedure (Figure 2E).


ATunable Scaffold of Microtubular Graphite for 3D Cell Growth
PEG-lipid functionalized AG was subjected tosupercritical point drying followed by deposition of a thin layerof gold. (A–C) Gradual zoom-in reveals the adsorbed PEG-lipidmolecules at high magnification. (D) A 4:1 mixture of amine terminated(top) and cyclic RGD peptide (cRGD) functionalized PEG-lipids (bottom)was used to promote cell attachment by integrin-mediated binding tocRGD. (E) Gold-coated pristine AG exhibits a smooth surface.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940076&req=5

fig2: PEG-lipid functionalized AG was subjected tosupercritical point drying followed by deposition of a thin layerof gold. (A–C) Gradual zoom-in reveals the adsorbed PEG-lipidmolecules at high magnification. (D) A 4:1 mixture of amine terminated(top) and cyclic RGD peptide (cRGD) functionalized PEG-lipids (bottom)was used to promote cell attachment by integrin-mediated binding tocRGD. (E) Gold-coated pristine AG exhibits a smooth surface.
Mentions: Scanning electron microscopy (SEM) was performed to verify adsorptionof PEG-lipids on the outer surface of the filaments (Figure 2). AG samples were dehydratedby super critical point drying (CPD) to avoid the destructive effectof surface tension on the network during evaporation of the liquidand a thin layer of gold was applied to improve visualization of biomoleculeson the graphitic filaments. The PEG-lipids became visible at highmagnification as bright dots (Figure 2B, C), and were found to decorate the surface as adense and homogeneous monolayer of individual molecules. Control experimentswith gold-coated pristine AG confirmed that the observed nanostructureswere not artifacts of the coating procedure (Figure 2E).

View Article: PubMed Central - PubMed

ABSTRACT

Aerographite(AG) is a novel carbon-based material that exists as a self-supportive3D network of interconnected hollow microtubules. It can be synthesizedin a variety of architectures tailored by the growth conditions. Thisflexibility in creating structures presents interesting bioengineeringpossibilities such as the generation of an artificial extracellularmatrix. Here we have explored the feasibility and potential of AGas a scaffold for 3D cell growth employing cyclic RGD (cRGD) peptidescoupled to poly(ethylene glycol) (PEG) conjugated phospholipids forsurface functionalization to promote specific adhesion of fibroblastcells. Successful growth and invasion of the bulk material was followedover a period of 4 days.

No MeSH data available.