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Overexpression of neuromedin U is correlated with regional metastasis of head and neck squamous cell carcinoma.

Wang L, Chen C, Li F, Hua QQ, Chen S, Xiao B, Dai M, Li M, Zheng A, Yu D, Hu ZW, Tao Z - Mol Med Rep (2016)

Bottom Line: The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis.There was increased protein expression of Nmu in the advanced tumor tissues.The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Regional metastasis is an important prognostic factor for patients with head and neck squamous cell carcinoma (HNSCC). Neuromedin U (Nmu) is a secreted neuropeptide, named due to its potent uterine contraction‑inducing activity. The aim of the present study was to analyze the significance of Nmu in the regional metastasis of HNSCC. The characteristics of 240 patients recruited from the Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University (Wuhan, China) were summarized retrospectively. The positive rate of neck dissection was analyzed according to the material. The expression levels of Nmu in human tumor samples were analyzed using immunohistochemistry. Subsequently, the expression of Nmu was investigated using a tissue microassay to analyze the association between Nmu protein expression and Tumor Node Metastasis (TNM) status. The positive rate of neck dissection was 51.4% in the study sample. The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis. There was increased protein expression of Nmu in the advanced tumor tissues. The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status. Overexpression of Nmu may be involved in the process of regional metastasis of HNSCC, and may serve as a novel and valuable biomarker for predicting regional metastasis in patients with HNSCC.

No MeSH data available.


Related in: MedlinePlus

(A) Representative images of IHC staining for Nmu protien in a tissue microassay of tumor tissues of different N-stages. Normal tissue stained negative for Nmu protein. N0, N1 and N2 stages showed weak positive, positive and strongly positive staining, respectively. (Images, magnification, ×100; enlarged area of image in top right corner, magnification, ×200). (B) IHC index (intensity x percentage of tumor cells). Expression levels of Nmu in the N0 tissues were significantly higher, compared with those in normal tissues. There was a statistically significant difference between N1 and N0, and the N2 index was significantly higher, compared with the N1 index Data are expressed as the mean ± standard deviation (*P<0.05 and **P<0.01). Nmu, neuromedin U; IHC, immunohistochemistry.
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f4-mmr-14-02-1075: (A) Representative images of IHC staining for Nmu protien in a tissue microassay of tumor tissues of different N-stages. Normal tissue stained negative for Nmu protein. N0, N1 and N2 stages showed weak positive, positive and strongly positive staining, respectively. (Images, magnification, ×100; enlarged area of image in top right corner, magnification, ×200). (B) IHC index (intensity x percentage of tumor cells). Expression levels of Nmu in the N0 tissues were significantly higher, compared with those in normal tissues. There was a statistically significant difference between N1 and N0, and the N2 index was significantly higher, compared with the N1 index Data are expressed as the mean ± standard deviation (*P<0.05 and **P<0.01). Nmu, neuromedin U; IHC, immunohistochemistry.

Mentions: The N-stage represents the lymph node metastasis. The present study analyzed the correlation between the N-stage and the expression levels of Nmu, for which representative images are shown in Fig. 4A. The CES of the Nmu protein showed that there was a statistically significant difference in the expression of Nmu in the N0 tissues, compared with normal tissues (P<0.05). There was also a statistically significant difference between the N1 and N0 tissues (P<0.01). In addition, a statistically significant difference in the expression of Nmu was observed between the N2 and N1 tissues (P<0.01; Fig. 4B).


Overexpression of neuromedin U is correlated with regional metastasis of head and neck squamous cell carcinoma.

Wang L, Chen C, Li F, Hua QQ, Chen S, Xiao B, Dai M, Li M, Zheng A, Yu D, Hu ZW, Tao Z - Mol Med Rep (2016)

(A) Representative images of IHC staining for Nmu protien in a tissue microassay of tumor tissues of different N-stages. Normal tissue stained negative for Nmu protein. N0, N1 and N2 stages showed weak positive, positive and strongly positive staining, respectively. (Images, magnification, ×100; enlarged area of image in top right corner, magnification, ×200). (B) IHC index (intensity x percentage of tumor cells). Expression levels of Nmu in the N0 tissues were significantly higher, compared with those in normal tissues. There was a statistically significant difference between N1 and N0, and the N2 index was significantly higher, compared with the N1 index Data are expressed as the mean ± standard deviation (*P<0.05 and **P<0.01). Nmu, neuromedin U; IHC, immunohistochemistry.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940074&req=5

f4-mmr-14-02-1075: (A) Representative images of IHC staining for Nmu protien in a tissue microassay of tumor tissues of different N-stages. Normal tissue stained negative for Nmu protein. N0, N1 and N2 stages showed weak positive, positive and strongly positive staining, respectively. (Images, magnification, ×100; enlarged area of image in top right corner, magnification, ×200). (B) IHC index (intensity x percentage of tumor cells). Expression levels of Nmu in the N0 tissues were significantly higher, compared with those in normal tissues. There was a statistically significant difference between N1 and N0, and the N2 index was significantly higher, compared with the N1 index Data are expressed as the mean ± standard deviation (*P<0.05 and **P<0.01). Nmu, neuromedin U; IHC, immunohistochemistry.
Mentions: The N-stage represents the lymph node metastasis. The present study analyzed the correlation between the N-stage and the expression levels of Nmu, for which representative images are shown in Fig. 4A. The CES of the Nmu protein showed that there was a statistically significant difference in the expression of Nmu in the N0 tissues, compared with normal tissues (P<0.05). There was also a statistically significant difference between the N1 and N0 tissues (P<0.01). In addition, a statistically significant difference in the expression of Nmu was observed between the N2 and N1 tissues (P<0.01; Fig. 4B).

Bottom Line: The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis.There was increased protein expression of Nmu in the advanced tumor tissues.The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Regional metastasis is an important prognostic factor for patients with head and neck squamous cell carcinoma (HNSCC). Neuromedin U (Nmu) is a secreted neuropeptide, named due to its potent uterine contraction‑inducing activity. The aim of the present study was to analyze the significance of Nmu in the regional metastasis of HNSCC. The characteristics of 240 patients recruited from the Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University (Wuhan, China) were summarized retrospectively. The positive rate of neck dissection was analyzed according to the material. The expression levels of Nmu in human tumor samples were analyzed using immunohistochemistry. Subsequently, the expression of Nmu was investigated using a tissue microassay to analyze the association between Nmu protein expression and Tumor Node Metastasis (TNM) status. The positive rate of neck dissection was 51.4% in the study sample. The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis. There was increased protein expression of Nmu in the advanced tumor tissues. The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status. Overexpression of Nmu may be involved in the process of regional metastasis of HNSCC, and may serve as a novel and valuable biomarker for predicting regional metastasis in patients with HNSCC.

No MeSH data available.


Related in: MedlinePlus