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Overexpression of neuromedin U is correlated with regional metastasis of head and neck squamous cell carcinoma.

Wang L, Chen C, Li F, Hua QQ, Chen S, Xiao B, Dai M, Li M, Zheng A, Yu D, Hu ZW, Tao Z - Mol Med Rep (2016)

Bottom Line: The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis.There was increased protein expression of Nmu in the advanced tumor tissues.The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Regional metastasis is an important prognostic factor for patients with head and neck squamous cell carcinoma (HNSCC). Neuromedin U (Nmu) is a secreted neuropeptide, named due to its potent uterine contraction‑inducing activity. The aim of the present study was to analyze the significance of Nmu in the regional metastasis of HNSCC. The characteristics of 240 patients recruited from the Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University (Wuhan, China) were summarized retrospectively. The positive rate of neck dissection was analyzed according to the material. The expression levels of Nmu in human tumor samples were analyzed using immunohistochemistry. Subsequently, the expression of Nmu was investigated using a tissue microassay to analyze the association between Nmu protein expression and Tumor Node Metastasis (TNM) status. The positive rate of neck dissection was 51.4% in the study sample. The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis. There was increased protein expression of Nmu in the advanced tumor tissues. The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status. Overexpression of Nmu may be involved in the process of regional metastasis of HNSCC, and may serve as a novel and valuable biomarker for predicting regional metastasis in patients with HNSCC.

No MeSH data available.


Related in: MedlinePlus

Representative images of IHC staining for Nmu protein in a tissue microassay of tissues at different T-stages. Normal tissue stained negative for Nmu protein. T1 showed weak positive staining, T2 stained positively, T3 and T4 showed strongly positive staining. (Image magnification, ×100; enlarged area in top right corner, magnification, ×200) IHC index (intensity x percentage of tumor cells) showed a statistically significant difference between T1 and normal tissues, and between T2 and T1 tissues. Data are expressed as the mean ± standard deviation (*P<0.05, **P<0.01). Nmu, neuromedin U; IHC, immunohistochemistry.
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f3-mmr-14-02-1075: Representative images of IHC staining for Nmu protein in a tissue microassay of tissues at different T-stages. Normal tissue stained negative for Nmu protein. T1 showed weak positive staining, T2 stained positively, T3 and T4 showed strongly positive staining. (Image magnification, ×100; enlarged area in top right corner, magnification, ×200) IHC index (intensity x percentage of tumor cells) showed a statistically significant difference between T1 and normal tissues, and between T2 and T1 tissues. Data are expressed as the mean ± standard deviation (*P<0.05, **P<0.01). Nmu, neuromedin U; IHC, immunohistochemistry.

Mentions: For the T-stage, representative images of the expression of Nmu are shown in Fig. 3. The protein expression of Nmu had a significantly increased CES in T1 tissues, compared with the normal tissues (P<0.05), and the CES in the T2, T3 and T4 tissues was markedly increased, compared with T1 tissues (P<0.01). There was a statistically significant difference between T3 and T2 tissues, however, no statistically significant differences were observed between T2 and T1 tissues or between T3 and T4 tissues, These data suggested that the expression of Nmu was correlated with T-stage, and an increase in the expression of Nmu was found in HNSCC at a high T-stage.


Overexpression of neuromedin U is correlated with regional metastasis of head and neck squamous cell carcinoma.

Wang L, Chen C, Li F, Hua QQ, Chen S, Xiao B, Dai M, Li M, Zheng A, Yu D, Hu ZW, Tao Z - Mol Med Rep (2016)

Representative images of IHC staining for Nmu protein in a tissue microassay of tissues at different T-stages. Normal tissue stained negative for Nmu protein. T1 showed weak positive staining, T2 stained positively, T3 and T4 showed strongly positive staining. (Image magnification, ×100; enlarged area in top right corner, magnification, ×200) IHC index (intensity x percentage of tumor cells) showed a statistically significant difference between T1 and normal tissues, and between T2 and T1 tissues. Data are expressed as the mean ± standard deviation (*P<0.05, **P<0.01). Nmu, neuromedin U; IHC, immunohistochemistry.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940074&req=5

f3-mmr-14-02-1075: Representative images of IHC staining for Nmu protein in a tissue microassay of tissues at different T-stages. Normal tissue stained negative for Nmu protein. T1 showed weak positive staining, T2 stained positively, T3 and T4 showed strongly positive staining. (Image magnification, ×100; enlarged area in top right corner, magnification, ×200) IHC index (intensity x percentage of tumor cells) showed a statistically significant difference between T1 and normal tissues, and between T2 and T1 tissues. Data are expressed as the mean ± standard deviation (*P<0.05, **P<0.01). Nmu, neuromedin U; IHC, immunohistochemistry.
Mentions: For the T-stage, representative images of the expression of Nmu are shown in Fig. 3. The protein expression of Nmu had a significantly increased CES in T1 tissues, compared with the normal tissues (P<0.05), and the CES in the T2, T3 and T4 tissues was markedly increased, compared with T1 tissues (P<0.01). There was a statistically significant difference between T3 and T2 tissues, however, no statistically significant differences were observed between T2 and T1 tissues or between T3 and T4 tissues, These data suggested that the expression of Nmu was correlated with T-stage, and an increase in the expression of Nmu was found in HNSCC at a high T-stage.

Bottom Line: The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis.There was increased protein expression of Nmu in the advanced tumor tissues.The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Regional metastasis is an important prognostic factor for patients with head and neck squamous cell carcinoma (HNSCC). Neuromedin U (Nmu) is a secreted neuropeptide, named due to its potent uterine contraction‑inducing activity. The aim of the present study was to analyze the significance of Nmu in the regional metastasis of HNSCC. The characteristics of 240 patients recruited from the Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University (Wuhan, China) were summarized retrospectively. The positive rate of neck dissection was analyzed according to the material. The expression levels of Nmu in human tumor samples were analyzed using immunohistochemistry. Subsequently, the expression of Nmu was investigated using a tissue microassay to analyze the association between Nmu protein expression and Tumor Node Metastasis (TNM) status. The positive rate of neck dissection was 51.4% in the study sample. The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis. There was increased protein expression of Nmu in the advanced tumor tissues. The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status. Overexpression of Nmu may be involved in the process of regional metastasis of HNSCC, and may serve as a novel and valuable biomarker for predicting regional metastasis in patients with HNSCC.

No MeSH data available.


Related in: MedlinePlus