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Overexpression of neuromedin U is correlated with regional metastasis of head and neck squamous cell carcinoma.

Wang L, Chen C, Li F, Hua QQ, Chen S, Xiao B, Dai M, Li M, Zheng A, Yu D, Hu ZW, Tao Z - Mol Med Rep (2016)

Bottom Line: The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis.There was increased protein expression of Nmu in the advanced tumor tissues.The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Regional metastasis is an important prognostic factor for patients with head and neck squamous cell carcinoma (HNSCC). Neuromedin U (Nmu) is a secreted neuropeptide, named due to its potent uterine contraction‑inducing activity. The aim of the present study was to analyze the significance of Nmu in the regional metastasis of HNSCC. The characteristics of 240 patients recruited from the Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University (Wuhan, China) were summarized retrospectively. The positive rate of neck dissection was analyzed according to the material. The expression levels of Nmu in human tumor samples were analyzed using immunohistochemistry. Subsequently, the expression of Nmu was investigated using a tissue microassay to analyze the association between Nmu protein expression and Tumor Node Metastasis (TNM) status. The positive rate of neck dissection was 51.4% in the study sample. The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis. There was increased protein expression of Nmu in the advanced tumor tissues. The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status. Overexpression of Nmu may be involved in the process of regional metastasis of HNSCC, and may serve as a novel and valuable biomarker for predicting regional metastasis in patients with HNSCC.

No MeSH data available.


Related in: MedlinePlus

(A) Representative images of IHC staining for Nmu protien in human primary tumors with metastasis and without metastasis (Magnification, ×200). (B) IHC index (intensity x percentage of tumor cells) showed that the expression of Nmu in the primary tumor tissues with metastasis was higher, compared with the tissues without metastasis (**P<0.01). Nmu, neuromedin U IHC, immunohistochemical.
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f1-mmr-14-02-1075: (A) Representative images of IHC staining for Nmu protien in human primary tumors with metastasis and without metastasis (Magnification, ×200). (B) IHC index (intensity x percentage of tumor cells) showed that the expression of Nmu in the primary tumor tissues with metastasis was higher, compared with the tissues without metastasis (**P<0.01). Nmu, neuromedin U IHC, immunohistochemical.

Mentions: The present study first identified the expression of Nmu in the clinical tissue sections of HNSCC. The frequency and intensity of the immunohistochemical staining in all tissue sections were analyzed. Representative images of the expression of Nmu in the tissues are shown in Fig. 1A. Nmu was expressed in the cytoplasmic membrane. The expression level of Nmu was transformed into a single numerical measurement, CES. A significant increase in CES was noted in the primary tumor with metastasis group, compared with the group without metastasis (P<0.01; Fig. 1B). From the images, it appeared that the Nmu protein was expressed predominantly among the carcinoma nests in primary tumors with metastasis. These data suggested that Nmu may be involved in the process of metastasis in HNSCC.


Overexpression of neuromedin U is correlated with regional metastasis of head and neck squamous cell carcinoma.

Wang L, Chen C, Li F, Hua QQ, Chen S, Xiao B, Dai M, Li M, Zheng A, Yu D, Hu ZW, Tao Z - Mol Med Rep (2016)

(A) Representative images of IHC staining for Nmu protien in human primary tumors with metastasis and without metastasis (Magnification, ×200). (B) IHC index (intensity x percentage of tumor cells) showed that the expression of Nmu in the primary tumor tissues with metastasis was higher, compared with the tissues without metastasis (**P<0.01). Nmu, neuromedin U IHC, immunohistochemical.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940074&req=5

f1-mmr-14-02-1075: (A) Representative images of IHC staining for Nmu protien in human primary tumors with metastasis and without metastasis (Magnification, ×200). (B) IHC index (intensity x percentage of tumor cells) showed that the expression of Nmu in the primary tumor tissues with metastasis was higher, compared with the tissues without metastasis (**P<0.01). Nmu, neuromedin U IHC, immunohistochemical.
Mentions: The present study first identified the expression of Nmu in the clinical tissue sections of HNSCC. The frequency and intensity of the immunohistochemical staining in all tissue sections were analyzed. Representative images of the expression of Nmu in the tissues are shown in Fig. 1A. Nmu was expressed in the cytoplasmic membrane. The expression level of Nmu was transformed into a single numerical measurement, CES. A significant increase in CES was noted in the primary tumor with metastasis group, compared with the group without metastasis (P<0.01; Fig. 1B). From the images, it appeared that the Nmu protein was expressed predominantly among the carcinoma nests in primary tumors with metastasis. These data suggested that Nmu may be involved in the process of metastasis in HNSCC.

Bottom Line: The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis.There was increased protein expression of Nmu in the advanced tumor tissues.The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Regional metastasis is an important prognostic factor for patients with head and neck squamous cell carcinoma (HNSCC). Neuromedin U (Nmu) is a secreted neuropeptide, named due to its potent uterine contraction‑inducing activity. The aim of the present study was to analyze the significance of Nmu in the regional metastasis of HNSCC. The characteristics of 240 patients recruited from the Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University (Wuhan, China) were summarized retrospectively. The positive rate of neck dissection was analyzed according to the material. The expression levels of Nmu in human tumor samples were analyzed using immunohistochemistry. Subsequently, the expression of Nmu was investigated using a tissue microassay to analyze the association between Nmu protein expression and Tumor Node Metastasis (TNM) status. The positive rate of neck dissection was 51.4% in the study sample. The expression levels of Nmu in primary tumors with regional metastasis were higher, compared with those without metastasis. There was increased protein expression of Nmu in the advanced tumor tissues. The data obtained in the present study demonstrated that the expression of Nmu was correlated with regional metastasis and TNM status. Overexpression of Nmu may be involved in the process of regional metastasis of HNSCC, and may serve as a novel and valuable biomarker for predicting regional metastasis in patients with HNSCC.

No MeSH data available.


Related in: MedlinePlus