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Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption.

Wang X, Fan F, Cao Q - Mol Med Rep (2016)

Bottom Line: Assessment of clinical colitis and histological examination found that the administration of modified Pulsatilla decoction attenuated the severity of oxazolone‑induced colitis in mice.Measurement of cytokine concentration, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated modified Pulsatilla decoction treatment significantly reduced the secretion of pro‑inflammatory cytokines and restored alterations in tight junction proteins in the colon tissues.Thus, the findings of the present study demonstrated that modified Pulsatilla decoction offers an effective therapeutic approach for the treatment of IBD and revealed the underlying mechanisms of action offered by modified Pulsatilla decoction.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China.

ABSTRACT
Inflammatory bowel diseases (IBDs) are chronic inflammatory gastrointestinal disorders caused by a dysregulated mucosal immune response and epithelial barrier disruption. Conventional treatment of IBD is currently limited to overcoming patient symptoms and is often associated with severe adverse effects from the drugs used. Modified Pulsatilla decoction has been used previously to treat ulcerative colitis (UC) in clinical practice in China, however, the underlying mechanism in the treatment of UC remains to be elucidated. In the present study, the efficiency and mechanisms of modified Pulsatilla decoction in the treatment of oxazolone‑induced colitis were investigated. Assessment of clinical colitis and histological examination found that the administration of modified Pulsatilla decoction attenuated the severity of oxazolone‑induced colitis in mice. Measurement of cytokine concentration, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated modified Pulsatilla decoction treatment significantly reduced the secretion of pro‑inflammatory cytokines and restored alterations in tight junction proteins in the colon tissues. In addition, modified Pulsatilla decoction suppressed the activation of the nuclear factor‑κB signaling pathway. Thus, the findings of the present study demonstrated that modified Pulsatilla decoction offers an effective therapeutic approach for the treatment of IBD and revealed the underlying mechanisms of action offered by modified Pulsatilla decoction.

No MeSH data available.


Related in: MedlinePlus

Modified Pulsatilla decoction treatment restores the alteration of tight junction proteins in colon epithelial cells. The expression levels of tight junction proteins, occludin, ZO-1 and claudin-2 in colonic epithelial cells of mice treated with or without modified Pulsatilla decoction or SASP were detected using western blot analysis. Data are expressed as the mean ± standard deviation. **P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; Con, control.
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f3-mmr-14-02-1173: Modified Pulsatilla decoction treatment restores the alteration of tight junction proteins in colon epithelial cells. The expression levels of tight junction proteins, occludin, ZO-1 and claudin-2 in colonic epithelial cells of mice treated with or without modified Pulsatilla decoction or SASP were detected using western blot analysis. Data are expressed as the mean ± standard deviation. **P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; Con, control.

Mentions: Dysregulated gut epithelial permeability, which is determined by the intercellular tight junctions in chronic intestinal inflammation, has been suggested as a primary defect in IBD. To determine whether modified Pulsatilla decoction treatment can protect the integrity of the intestinal barrier, the present study examined the expression levels of the tight junction proteins, occludin, ZO-1 and claudin-2 in the colonic epithelial cells of mice treated with or without modified Pulsatilla decoction or SASP. As expected, the intercellular tight junctions were disrupted in the colon epithelium of the oxazolone-induced colitis mice, which was demonstrated by decreased expression levels of the tight junction proteins, occludin and ZO-1, and increased expression of claudin-2. However, treatment with modified Pulsatilla decoction or SASP restored the altered expression levels of occludin, ZO-1 and claudin-2 in the colon epithelial cells of the oxazolone-induced colitis mice, almost to normal levels (Fig. 3). These results indicated that modified Pulsatilla decoction prevented the alteration of tight junction proteins caused by the inflammatory stimuli in colon epithelial cells.


Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption.

Wang X, Fan F, Cao Q - Mol Med Rep (2016)

Modified Pulsatilla decoction treatment restores the alteration of tight junction proteins in colon epithelial cells. The expression levels of tight junction proteins, occludin, ZO-1 and claudin-2 in colonic epithelial cells of mice treated with or without modified Pulsatilla decoction or SASP were detected using western blot analysis. Data are expressed as the mean ± standard deviation. **P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; Con, control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940073&req=5

f3-mmr-14-02-1173: Modified Pulsatilla decoction treatment restores the alteration of tight junction proteins in colon epithelial cells. The expression levels of tight junction proteins, occludin, ZO-1 and claudin-2 in colonic epithelial cells of mice treated with or without modified Pulsatilla decoction or SASP were detected using western blot analysis. Data are expressed as the mean ± standard deviation. **P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; Con, control.
Mentions: Dysregulated gut epithelial permeability, which is determined by the intercellular tight junctions in chronic intestinal inflammation, has been suggested as a primary defect in IBD. To determine whether modified Pulsatilla decoction treatment can protect the integrity of the intestinal barrier, the present study examined the expression levels of the tight junction proteins, occludin, ZO-1 and claudin-2 in the colonic epithelial cells of mice treated with or without modified Pulsatilla decoction or SASP. As expected, the intercellular tight junctions were disrupted in the colon epithelium of the oxazolone-induced colitis mice, which was demonstrated by decreased expression levels of the tight junction proteins, occludin and ZO-1, and increased expression of claudin-2. However, treatment with modified Pulsatilla decoction or SASP restored the altered expression levels of occludin, ZO-1 and claudin-2 in the colon epithelial cells of the oxazolone-induced colitis mice, almost to normal levels (Fig. 3). These results indicated that modified Pulsatilla decoction prevented the alteration of tight junction proteins caused by the inflammatory stimuli in colon epithelial cells.

Bottom Line: Assessment of clinical colitis and histological examination found that the administration of modified Pulsatilla decoction attenuated the severity of oxazolone‑induced colitis in mice.Measurement of cytokine concentration, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated modified Pulsatilla decoction treatment significantly reduced the secretion of pro‑inflammatory cytokines and restored alterations in tight junction proteins in the colon tissues.Thus, the findings of the present study demonstrated that modified Pulsatilla decoction offers an effective therapeutic approach for the treatment of IBD and revealed the underlying mechanisms of action offered by modified Pulsatilla decoction.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China.

ABSTRACT
Inflammatory bowel diseases (IBDs) are chronic inflammatory gastrointestinal disorders caused by a dysregulated mucosal immune response and epithelial barrier disruption. Conventional treatment of IBD is currently limited to overcoming patient symptoms and is often associated with severe adverse effects from the drugs used. Modified Pulsatilla decoction has been used previously to treat ulcerative colitis (UC) in clinical practice in China, however, the underlying mechanism in the treatment of UC remains to be elucidated. In the present study, the efficiency and mechanisms of modified Pulsatilla decoction in the treatment of oxazolone‑induced colitis were investigated. Assessment of clinical colitis and histological examination found that the administration of modified Pulsatilla decoction attenuated the severity of oxazolone‑induced colitis in mice. Measurement of cytokine concentration, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated modified Pulsatilla decoction treatment significantly reduced the secretion of pro‑inflammatory cytokines and restored alterations in tight junction proteins in the colon tissues. In addition, modified Pulsatilla decoction suppressed the activation of the nuclear factor‑κB signaling pathway. Thus, the findings of the present study demonstrated that modified Pulsatilla decoction offers an effective therapeutic approach for the treatment of IBD and revealed the underlying mechanisms of action offered by modified Pulsatilla decoction.

No MeSH data available.


Related in: MedlinePlus