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Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption.

Wang X, Fan F, Cao Q - Mol Med Rep (2016)

Bottom Line: Assessment of clinical colitis and histological examination found that the administration of modified Pulsatilla decoction attenuated the severity of oxazolone‑induced colitis in mice.Measurement of cytokine concentration, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated modified Pulsatilla decoction treatment significantly reduced the secretion of pro‑inflammatory cytokines and restored alterations in tight junction proteins in the colon tissues.Thus, the findings of the present study demonstrated that modified Pulsatilla decoction offers an effective therapeutic approach for the treatment of IBD and revealed the underlying mechanisms of action offered by modified Pulsatilla decoction.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China.

ABSTRACT
Inflammatory bowel diseases (IBDs) are chronic inflammatory gastrointestinal disorders caused by a dysregulated mucosal immune response and epithelial barrier disruption. Conventional treatment of IBD is currently limited to overcoming patient symptoms and is often associated with severe adverse effects from the drugs used. Modified Pulsatilla decoction has been used previously to treat ulcerative colitis (UC) in clinical practice in China, however, the underlying mechanism in the treatment of UC remains to be elucidated. In the present study, the efficiency and mechanisms of modified Pulsatilla decoction in the treatment of oxazolone‑induced colitis were investigated. Assessment of clinical colitis and histological examination found that the administration of modified Pulsatilla decoction attenuated the severity of oxazolone‑induced colitis in mice. Measurement of cytokine concentration, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated modified Pulsatilla decoction treatment significantly reduced the secretion of pro‑inflammatory cytokines and restored alterations in tight junction proteins in the colon tissues. In addition, modified Pulsatilla decoction suppressed the activation of the nuclear factor‑κB signaling pathway. Thus, the findings of the present study demonstrated that modified Pulsatilla decoction offers an effective therapeutic approach for the treatment of IBD and revealed the underlying mechanisms of action offered by modified Pulsatilla decoction.

No MeSH data available.


Related in: MedlinePlus

Modified Pulsatilla decoction treatment significantly reduces the secretion of pro-inflammatory cytokines. (A) mRNA expression levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using reverse transcription-quantitative polymerase chain reaction analysis. (B) Protein levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using ELISA. Data are expressed as the mean ± standard deviation and are representative of three independent experiments. *P<0.05, **P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; IL, interleukin.
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f2-mmr-14-02-1173: Modified Pulsatilla decoction treatment significantly reduces the secretion of pro-inflammatory cytokines. (A) mRNA expression levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using reverse transcription-quantitative polymerase chain reaction analysis. (B) Protein levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using ELISA. Data are expressed as the mean ± standard deviation and are representative of three independent experiments. *P<0.05, **P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; IL, interleukin.

Mentions: As the excessive production of pro-inflammatory cytokines is closely correlated with tissue damage and is critical in the pathogenesis of IBD (24), the present study characterized the expression levels of inflammatory cytokines in the colonic mucosal tissues. As shown in Fig. 2A and B, the expression levels of IL-5 and IL-13 were low in the colon tissues of the normal mice, and markedly upregulated in the colon tissues of the oxazolone-induced colitis mice. Following the administration of modified Pulsatilla decoction or SASP, the elevated expression levels of colonic IL-5 and IL-13 were significantly attenuated. However, no significant differences were detected in the expression levels of colonic IL-5 and IL-13 between the modified Pulsatilla decoction and SASP-treated mice. Therefore, these results suggested that Pulsatilla decoction exerted anti-inflammatory effects in oxazolone-induced colitis.


Modified Pulsatilla decoction attenuates oxazolone-induced colitis in mice through suppression of inflammation and epithelial barrier disruption.

Wang X, Fan F, Cao Q - Mol Med Rep (2016)

Modified Pulsatilla decoction treatment significantly reduces the secretion of pro-inflammatory cytokines. (A) mRNA expression levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using reverse transcription-quantitative polymerase chain reaction analysis. (B) Protein levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using ELISA. Data are expressed as the mean ± standard deviation and are representative of three independent experiments. *P<0.05, **P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; IL, interleukin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940073&req=5

f2-mmr-14-02-1173: Modified Pulsatilla decoction treatment significantly reduces the secretion of pro-inflammatory cytokines. (A) mRNA expression levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using reverse transcription-quantitative polymerase chain reaction analysis. (B) Protein levels of IL-5 and IL-13 in colon tissues from oxazolone-induced colitis mice, following treatment with or without modified Pulsatilla decoction or SASP, were determined using ELISA. Data are expressed as the mean ± standard deviation and are representative of three independent experiments. *P<0.05, **P<0.01 vs. the control group. PBS, phosphate-buffered saline; SASP, sulfasalazine; IL, interleukin.
Mentions: As the excessive production of pro-inflammatory cytokines is closely correlated with tissue damage and is critical in the pathogenesis of IBD (24), the present study characterized the expression levels of inflammatory cytokines in the colonic mucosal tissues. As shown in Fig. 2A and B, the expression levels of IL-5 and IL-13 were low in the colon tissues of the normal mice, and markedly upregulated in the colon tissues of the oxazolone-induced colitis mice. Following the administration of modified Pulsatilla decoction or SASP, the elevated expression levels of colonic IL-5 and IL-13 were significantly attenuated. However, no significant differences were detected in the expression levels of colonic IL-5 and IL-13 between the modified Pulsatilla decoction and SASP-treated mice. Therefore, these results suggested that Pulsatilla decoction exerted anti-inflammatory effects in oxazolone-induced colitis.

Bottom Line: Assessment of clinical colitis and histological examination found that the administration of modified Pulsatilla decoction attenuated the severity of oxazolone‑induced colitis in mice.Measurement of cytokine concentration, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated modified Pulsatilla decoction treatment significantly reduced the secretion of pro‑inflammatory cytokines and restored alterations in tight junction proteins in the colon tissues.Thus, the findings of the present study demonstrated that modified Pulsatilla decoction offers an effective therapeutic approach for the treatment of IBD and revealed the underlying mechanisms of action offered by modified Pulsatilla decoction.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China.

ABSTRACT
Inflammatory bowel diseases (IBDs) are chronic inflammatory gastrointestinal disorders caused by a dysregulated mucosal immune response and epithelial barrier disruption. Conventional treatment of IBD is currently limited to overcoming patient symptoms and is often associated with severe adverse effects from the drugs used. Modified Pulsatilla decoction has been used previously to treat ulcerative colitis (UC) in clinical practice in China, however, the underlying mechanism in the treatment of UC remains to be elucidated. In the present study, the efficiency and mechanisms of modified Pulsatilla decoction in the treatment of oxazolone‑induced colitis were investigated. Assessment of clinical colitis and histological examination found that the administration of modified Pulsatilla decoction attenuated the severity of oxazolone‑induced colitis in mice. Measurement of cytokine concentration, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated modified Pulsatilla decoction treatment significantly reduced the secretion of pro‑inflammatory cytokines and restored alterations in tight junction proteins in the colon tissues. In addition, modified Pulsatilla decoction suppressed the activation of the nuclear factor‑κB signaling pathway. Thus, the findings of the present study demonstrated that modified Pulsatilla decoction offers an effective therapeutic approach for the treatment of IBD and revealed the underlying mechanisms of action offered by modified Pulsatilla decoction.

No MeSH data available.


Related in: MedlinePlus