Limits...
Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression.

Wei Z, Yan L, Chen Y, Bao C, Deng J, Deng J - Mol Med Rep (2016)

Bottom Line: Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves.The results of the present study demonstrated that mangiferin significantly inhibits LPS/IFN‑γ stimulation‑induced classical activation of macrophages in vitro and markedly decreases proinflammatory cytokine release.In addition, cellular IRF5 expression was markedly downregulated.

View Article: PubMed Central - PubMed

Affiliation: Guangxi Scientific Experimental Center of Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China.

ABSTRACT
Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves. For hundreds of years, Mangifera indica Linn. leaf has been used as an ingredient in numerous traditional Chinese medicine preparations for the treatment of bronchitis. However, the pharmacological mechanism of mangiferin in the treatment of bronchitis remains to be elucidated. Macrophage classical activation is important role in the process of bronchial airway inflammation, and interferon regulatory factor 5 (IRF5) has been identified as a key regulatory factor for macrophage classical activation. The present study used the THP‑1 human monocyte cell line to investigate whether mangiferin inhibits macrophage classical activation via suppressing IRF5 expression in vitro. THP‑1 cells were differentiated to macrophages by phorbol 12‑myristate 13‑acetate. Macrophages were polarized to M1 macrophages following stimulation with lipopolysaccharide (LPS)/interferon‑γ (IFN‑γ). Flow cytometric analysis was conducted to detect the M1 macrophages. Reverse transcription‑quantitative polymerase chain reaction was used to investigate cellular IRF5 gene expression. Levels of proinflammatory cytokines and IRF5 were assessed following cell culture and cellular homogenization using enzyme‑linked immunosorbent assay. IRF5 protein and nuclei co‑localization was performed in macrophages with laser scanning confocal microscope immunofluorescence analysis. The results of the present study demonstrated that mangiferin significantly inhibits LPS/IFN‑γ stimulation‑induced classical activation of macrophages in vitro and markedly decreases proinflammatory cytokine release. In addition, cellular IRF5 expression was markedly downregulated. These results suggest that the inhibitory effect of mangiferin on classical activation of macrophages may be exerted via downregulation of cellular IRF5 expression levels.

No MeSH data available.


Related in: MedlinePlus

High performance liquid chromatograms of (A) mangiferin standard substance and (B) sample. AU, arbitrary units.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940072&req=5

f2-mmr-14-02-1091: High performance liquid chromatograms of (A) mangiferin standard substance and (B) sample. AU, arbitrary units.

Mentions: Mangiferin was provided by the Key Laboratory of Traditional Chinese Medical Pharmacology, Guangxi University of Chinese Medicine (Nanning, China). It was isolated from leaves of Mangifera indica Linn. harvested in the Baise region of China. The preparation method was performed as described previously (19). At the end of the extraction process, a yellow powder with 97.5% purity was obtained (Fig. 1). Purity of the mangiferin powder was detected by high performance liquid chromatography (Fig. 2) (20). The HPLC measurement was performed on an Agilent 1100 LC system (Agilent, Santa Clara, CA, USA) and Elite Hypersil C18 column (5 µm, 4.6mmIDx250mm), with a gradient system of acetonitrile-0.1% phosphoric acid solution as a mobile phase at a flow rate of 1.0 ml/min. The detective wavelength was 258 nm, the column temperature was 30°C.


Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression.

Wei Z, Yan L, Chen Y, Bao C, Deng J, Deng J - Mol Med Rep (2016)

High performance liquid chromatograms of (A) mangiferin standard substance and (B) sample. AU, arbitrary units.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940072&req=5

f2-mmr-14-02-1091: High performance liquid chromatograms of (A) mangiferin standard substance and (B) sample. AU, arbitrary units.
Mentions: Mangiferin was provided by the Key Laboratory of Traditional Chinese Medical Pharmacology, Guangxi University of Chinese Medicine (Nanning, China). It was isolated from leaves of Mangifera indica Linn. harvested in the Baise region of China. The preparation method was performed as described previously (19). At the end of the extraction process, a yellow powder with 97.5% purity was obtained (Fig. 1). Purity of the mangiferin powder was detected by high performance liquid chromatography (Fig. 2) (20). The HPLC measurement was performed on an Agilent 1100 LC system (Agilent, Santa Clara, CA, USA) and Elite Hypersil C18 column (5 µm, 4.6mmIDx250mm), with a gradient system of acetonitrile-0.1% phosphoric acid solution as a mobile phase at a flow rate of 1.0 ml/min. The detective wavelength was 258 nm, the column temperature was 30°C.

Bottom Line: Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves.The results of the present study demonstrated that mangiferin significantly inhibits LPS/IFN‑γ stimulation‑induced classical activation of macrophages in vitro and markedly decreases proinflammatory cytokine release.In addition, cellular IRF5 expression was markedly downregulated.

View Article: PubMed Central - PubMed

Affiliation: Guangxi Scientific Experimental Center of Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China.

ABSTRACT
Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves. For hundreds of years, Mangifera indica Linn. leaf has been used as an ingredient in numerous traditional Chinese medicine preparations for the treatment of bronchitis. However, the pharmacological mechanism of mangiferin in the treatment of bronchitis remains to be elucidated. Macrophage classical activation is important role in the process of bronchial airway inflammation, and interferon regulatory factor 5 (IRF5) has been identified as a key regulatory factor for macrophage classical activation. The present study used the THP‑1 human monocyte cell line to investigate whether mangiferin inhibits macrophage classical activation via suppressing IRF5 expression in vitro. THP‑1 cells were differentiated to macrophages by phorbol 12‑myristate 13‑acetate. Macrophages were polarized to M1 macrophages following stimulation with lipopolysaccharide (LPS)/interferon‑γ (IFN‑γ). Flow cytometric analysis was conducted to detect the M1 macrophages. Reverse transcription‑quantitative polymerase chain reaction was used to investigate cellular IRF5 gene expression. Levels of proinflammatory cytokines and IRF5 were assessed following cell culture and cellular homogenization using enzyme‑linked immunosorbent assay. IRF5 protein and nuclei co‑localization was performed in macrophages with laser scanning confocal microscope immunofluorescence analysis. The results of the present study demonstrated that mangiferin significantly inhibits LPS/IFN‑γ stimulation‑induced classical activation of macrophages in vitro and markedly decreases proinflammatory cytokine release. In addition, cellular IRF5 expression was markedly downregulated. These results suggest that the inhibitory effect of mangiferin on classical activation of macrophages may be exerted via downregulation of cellular IRF5 expression levels.

No MeSH data available.


Related in: MedlinePlus