Limits...
Oncogenic role of microRNA‑20a in human uveal melanoma.

Zhou J, Jiang J, Wang S, Xia X - Mol Med Rep (2016)

Bottom Line: The expression levels of miR‑20a were significantly increased in the UM cells and tissues (P<0.05).By contrast, miR‑20a inhibition markedly suppressed the viability and motility of UM cells in vitro.These data provided convincing evidence that miR‑20a may function as an oncogenic miRNA, and may be involved in promoting cell growth and motility in the molecular etiology of UM, suggesting its potential as a candidate therapeutic target for the treatment of patients with UM.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.

ABSTRACT
As a member of the microRNA (miR)-17-92 cluster, miR‑20a has been indicated to be involved in the regulation of the proliferation and invasion of various cancer cells. Previous studies have observed elevated plasma levels of miR‑20a in patients with uveal melanoma (UM), compared with normal controls. In the present study, the potential function of miR‑20a in UM was investigated. Reverse transcription‑quantitative polymerase chain reaction analysis was performed to detect the expression levels of miR‑20a in UM cells and tissues. The functions of miR‑20a on cell proliferation, migration and invasion were determined in vitro using 3‑(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays, respectively. The expression levels of miR‑20a were significantly increased in the UM cells and tissues (P<0.05). Subsequently, miR‑20a mimics were transfected into UM cells, which led to increases in cell growth, migration and invasion activities. By contrast, miR‑20a inhibition markedly suppressed the viability and motility of UM cells in vitro. These data provided convincing evidence that miR‑20a may function as an oncogenic miRNA, and may be involved in promoting cell growth and motility in the molecular etiology of UM, suggesting its potential as a candidate therapeutic target for the treatment of patients with UM.

No MeSH data available.


Related in: MedlinePlus

miR-20a is upregulated in human UM tissues and cells. (A) In the human UM tissues, the mean expression level of miR-20a was 5.29 (range, 3.16–8.61). In the normal uveal tissues, the mean expression level of miR-20a was 2.39 (range, 0.68–4.47). Statistical analysis showed that the expression level of miR-20a in the UM tissues was significantly higher, compared with that in normal uveal tissues (P=0.001). (B) Expression levels of miR-20a were significantly increased in the MUM-2B and MUM-2C UM cell lines, compared with the D78 normal human melanocyte cell line (P=0.01). Data are presented as the mean ± standard deviation. UM, uveal melanoma; miR, microRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4940053&req=5

f1-mmr-14-02-1560: miR-20a is upregulated in human UM tissues and cells. (A) In the human UM tissues, the mean expression level of miR-20a was 5.29 (range, 3.16–8.61). In the normal uveal tissues, the mean expression level of miR-20a was 2.39 (range, 0.68–4.47). Statistical analysis showed that the expression level of miR-20a in the UM tissues was significantly higher, compared with that in normal uveal tissues (P=0.001). (B) Expression levels of miR-20a were significantly increased in the MUM-2B and MUM-2C UM cell lines, compared with the D78 normal human melanocyte cell line (P=0.01). Data are presented as the mean ± standard deviation. UM, uveal melanoma; miR, microRNA.

Mentions: In the human UM tissue samples, the mean expression level of miR-20a was 5.29 (range, 3.16–8.61). For the normal uveal tissues, the mean expression level of miR-20a was 2.39 (range, 0.68–4.47). Statistical analysis showed that the expression level of miR-20a in the UM tissues was significantly higher, compared with that in the normal uveal tissues (P=0.001; Fig. 1A). Similarly, the expression of miR-20a was also mark edly increased in the UM cell lines (MUM-2B and MUM-2C), compared with the normal human melanocyte D78 cell line (P= 0.01; Fig. 1B).


Oncogenic role of microRNA‑20a in human uveal melanoma.

Zhou J, Jiang J, Wang S, Xia X - Mol Med Rep (2016)

miR-20a is upregulated in human UM tissues and cells. (A) In the human UM tissues, the mean expression level of miR-20a was 5.29 (range, 3.16–8.61). In the normal uveal tissues, the mean expression level of miR-20a was 2.39 (range, 0.68–4.47). Statistical analysis showed that the expression level of miR-20a in the UM tissues was significantly higher, compared with that in normal uveal tissues (P=0.001). (B) Expression levels of miR-20a were significantly increased in the MUM-2B and MUM-2C UM cell lines, compared with the D78 normal human melanocyte cell line (P=0.01). Data are presented as the mean ± standard deviation. UM, uveal melanoma; miR, microRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4940053&req=5

f1-mmr-14-02-1560: miR-20a is upregulated in human UM tissues and cells. (A) In the human UM tissues, the mean expression level of miR-20a was 5.29 (range, 3.16–8.61). In the normal uveal tissues, the mean expression level of miR-20a was 2.39 (range, 0.68–4.47). Statistical analysis showed that the expression level of miR-20a in the UM tissues was significantly higher, compared with that in normal uveal tissues (P=0.001). (B) Expression levels of miR-20a were significantly increased in the MUM-2B and MUM-2C UM cell lines, compared with the D78 normal human melanocyte cell line (P=0.01). Data are presented as the mean ± standard deviation. UM, uveal melanoma; miR, microRNA.
Mentions: In the human UM tissue samples, the mean expression level of miR-20a was 5.29 (range, 3.16–8.61). For the normal uveal tissues, the mean expression level of miR-20a was 2.39 (range, 0.68–4.47). Statistical analysis showed that the expression level of miR-20a in the UM tissues was significantly higher, compared with that in the normal uveal tissues (P=0.001; Fig. 1A). Similarly, the expression of miR-20a was also mark edly increased in the UM cell lines (MUM-2B and MUM-2C), compared with the normal human melanocyte D78 cell line (P= 0.01; Fig. 1B).

Bottom Line: The expression levels of miR‑20a were significantly increased in the UM cells and tissues (P<0.05).By contrast, miR‑20a inhibition markedly suppressed the viability and motility of UM cells in vitro.These data provided convincing evidence that miR‑20a may function as an oncogenic miRNA, and may be involved in promoting cell growth and motility in the molecular etiology of UM, suggesting its potential as a candidate therapeutic target for the treatment of patients with UM.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.

ABSTRACT
As a member of the microRNA (miR)-17-92 cluster, miR‑20a has been indicated to be involved in the regulation of the proliferation and invasion of various cancer cells. Previous studies have observed elevated plasma levels of miR‑20a in patients with uveal melanoma (UM), compared with normal controls. In the present study, the potential function of miR‑20a in UM was investigated. Reverse transcription‑quantitative polymerase chain reaction analysis was performed to detect the expression levels of miR‑20a in UM cells and tissues. The functions of miR‑20a on cell proliferation, migration and invasion were determined in vitro using 3‑(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays, respectively. The expression levels of miR‑20a were significantly increased in the UM cells and tissues (P<0.05). Subsequently, miR‑20a mimics were transfected into UM cells, which led to increases in cell growth, migration and invasion activities. By contrast, miR‑20a inhibition markedly suppressed the viability and motility of UM cells in vitro. These data provided convincing evidence that miR‑20a may function as an oncogenic miRNA, and may be involved in promoting cell growth and motility in the molecular etiology of UM, suggesting its potential as a candidate therapeutic target for the treatment of patients with UM.

No MeSH data available.


Related in: MedlinePlus