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Effect of evodiamine and berberine on miR-429 as an oncogene in human colorectal cancer.

Liu H, Huang C, Wu L, Wen B - Onco Targets Ther (2016)

Bottom Line: Our results suggested that E-cadherin and Par3 were remarkably decreased in tumor tissues compared with those in normal tissues, and miR-429 was upregulated in tumor tissues.After treatment of BER and EVO, the level of miR-429 was lower in tumor tissues than in normal tissues.The data suggested that BER and EVO can be potential therapeutic agents for CRC, as they downregulated the expression level of miR-429.

View Article: PubMed Central - PubMed

Affiliation: Institute of Spleen and Stomach, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT
Loss of epithelial organization and deregulated microRNAs are hallmarks of malignant carcinomas, but the relationship between them has been poorly understood. This study was designed to investigate the changes in the expression of E-cadherin, Par3, and miR-429 during the development of human colorectal cancer (CRC). E-cadherin and Par3 levels were quantitatively detected by immunohistochemistry and Western blotting. An in vitro culture of colorectal tissue was established to analyze the effect of berberine (BER) and evodiamine (EVO) on the level of miR-429. Our results suggested that E-cadherin and Par3 were remarkably decreased in tumor tissues compared with those in normal tissues, and miR-429 was upregulated in tumor tissues. After treatment of BER and EVO, the level of miR-429 was lower in tumor tissues than in normal tissues. This study investigated the potential relationship between miR-429, E-cadherin, and Par3 in CRC. The data suggested that BER and EVO can be potential therapeutic agents for CRC, as they downregulated the expression level of miR-429.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry, Western blotting and qRT-PCR of E-cadherin and Par3.Notes: (A1 and A2) H&E staining of normal and tumor tissue (×200). (B1 and B2) Immunohistochemical staining of E-cadherin between normal and tumor tissues (×400). (C1 and C2) Immunohistochemical staining of Par3 between normal and tumor tissues (×400). (D1 and D2) Calcein-AM/PI staining of cultured tissue for 3 and 25 days (×40). (E) Western blotting of E-cadherin and Par3 between normal and tumor tissues. (F) Expression level of E-cadherin and Par3 analyzed by Western blotting. *Denotes statistical significance compared with normal tissues.Abbreviations: H&E, hematoxylin and eosin; PI, propidium iodide; AM, acetoxymethyl ester.
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f2-ott-9-4121: Immunohistochemistry, Western blotting and qRT-PCR of E-cadherin and Par3.Notes: (A1 and A2) H&E staining of normal and tumor tissue (×200). (B1 and B2) Immunohistochemical staining of E-cadherin between normal and tumor tissues (×400). (C1 and C2) Immunohistochemical staining of Par3 between normal and tumor tissues (×400). (D1 and D2) Calcein-AM/PI staining of cultured tissue for 3 and 25 days (×40). (E) Western blotting of E-cadherin and Par3 between normal and tumor tissues. (F) Expression level of E-cadherin and Par3 analyzed by Western blotting. *Denotes statistical significance compared with normal tissues.Abbreviations: H&E, hematoxylin and eosin; PI, propidium iodide; AM, acetoxymethyl ester.

Mentions: One of the earliest histopathological phenomena of CRC is aberrant crypt foci (ACF). Increasing evidence supported that ACF is the early indication of colon cancer.23 The size and number of ACF contributes to CRC development. Normal colon crypts were cut vertically to observe their histopathological characteristics. The colon crypts without branching or protuberances were test tube shaped and smooth on the surface. Compared to normal tissues, the crypts tumor tissues were losing their morphology (Figure 2A).


Effect of evodiamine and berberine on miR-429 as an oncogene in human colorectal cancer.

Liu H, Huang C, Wu L, Wen B - Onco Targets Ther (2016)

Immunohistochemistry, Western blotting and qRT-PCR of E-cadherin and Par3.Notes: (A1 and A2) H&E staining of normal and tumor tissue (×200). (B1 and B2) Immunohistochemical staining of E-cadherin between normal and tumor tissues (×400). (C1 and C2) Immunohistochemical staining of Par3 between normal and tumor tissues (×400). (D1 and D2) Calcein-AM/PI staining of cultured tissue for 3 and 25 days (×40). (E) Western blotting of E-cadherin and Par3 between normal and tumor tissues. (F) Expression level of E-cadherin and Par3 analyzed by Western blotting. *Denotes statistical significance compared with normal tissues.Abbreviations: H&E, hematoxylin and eosin; PI, propidium iodide; AM, acetoxymethyl ester.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940014&req=5

f2-ott-9-4121: Immunohistochemistry, Western blotting and qRT-PCR of E-cadherin and Par3.Notes: (A1 and A2) H&E staining of normal and tumor tissue (×200). (B1 and B2) Immunohistochemical staining of E-cadherin between normal and tumor tissues (×400). (C1 and C2) Immunohistochemical staining of Par3 between normal and tumor tissues (×400). (D1 and D2) Calcein-AM/PI staining of cultured tissue for 3 and 25 days (×40). (E) Western blotting of E-cadherin and Par3 between normal and tumor tissues. (F) Expression level of E-cadherin and Par3 analyzed by Western blotting. *Denotes statistical significance compared with normal tissues.Abbreviations: H&E, hematoxylin and eosin; PI, propidium iodide; AM, acetoxymethyl ester.
Mentions: One of the earliest histopathological phenomena of CRC is aberrant crypt foci (ACF). Increasing evidence supported that ACF is the early indication of colon cancer.23 The size and number of ACF contributes to CRC development. Normal colon crypts were cut vertically to observe their histopathological characteristics. The colon crypts without branching or protuberances were test tube shaped and smooth on the surface. Compared to normal tissues, the crypts tumor tissues were losing their morphology (Figure 2A).

Bottom Line: Our results suggested that E-cadherin and Par3 were remarkably decreased in tumor tissues compared with those in normal tissues, and miR-429 was upregulated in tumor tissues.After treatment of BER and EVO, the level of miR-429 was lower in tumor tissues than in normal tissues.The data suggested that BER and EVO can be potential therapeutic agents for CRC, as they downregulated the expression level of miR-429.

View Article: PubMed Central - PubMed

Affiliation: Institute of Spleen and Stomach, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT
Loss of epithelial organization and deregulated microRNAs are hallmarks of malignant carcinomas, but the relationship between them has been poorly understood. This study was designed to investigate the changes in the expression of E-cadherin, Par3, and miR-429 during the development of human colorectal cancer (CRC). E-cadherin and Par3 levels were quantitatively detected by immunohistochemistry and Western blotting. An in vitro culture of colorectal tissue was established to analyze the effect of berberine (BER) and evodiamine (EVO) on the level of miR-429. Our results suggested that E-cadherin and Par3 were remarkably decreased in tumor tissues compared with those in normal tissues, and miR-429 was upregulated in tumor tissues. After treatment of BER and EVO, the level of miR-429 was lower in tumor tissues than in normal tissues. This study investigated the potential relationship between miR-429, E-cadherin, and Par3 in CRC. The data suggested that BER and EVO can be potential therapeutic agents for CRC, as they downregulated the expression level of miR-429.

No MeSH data available.


Related in: MedlinePlus