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Effect of evodiamine and berberine on miR-429 as an oncogene in human colorectal cancer.

Liu H, Huang C, Wu L, Wen B - Onco Targets Ther (2016)

Bottom Line: Our results suggested that E-cadherin and Par3 were remarkably decreased in tumor tissues compared with those in normal tissues, and miR-429 was upregulated in tumor tissues.After treatment of BER and EVO, the level of miR-429 was lower in tumor tissues than in normal tissues.The data suggested that BER and EVO can be potential therapeutic agents for CRC, as they downregulated the expression level of miR-429.

View Article: PubMed Central - PubMed

Affiliation: Institute of Spleen and Stomach, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT
Loss of epithelial organization and deregulated microRNAs are hallmarks of malignant carcinomas, but the relationship between them has been poorly understood. This study was designed to investigate the changes in the expression of E-cadherin, Par3, and miR-429 during the development of human colorectal cancer (CRC). E-cadherin and Par3 levels were quantitatively detected by immunohistochemistry and Western blotting. An in vitro culture of colorectal tissue was established to analyze the effect of berberine (BER) and evodiamine (EVO) on the level of miR-429. Our results suggested that E-cadherin and Par3 were remarkably decreased in tumor tissues compared with those in normal tissues, and miR-429 was upregulated in tumor tissues. After treatment of BER and EVO, the level of miR-429 was lower in tumor tissues than in normal tissues. This study investigated the potential relationship between miR-429, E-cadherin, and Par3 in CRC. The data suggested that BER and EVO can be potential therapeutic agents for CRC, as they downregulated the expression level of miR-429.

No MeSH data available.


Related in: MedlinePlus

A network of target miRNAs–messenger RNAs. Blue nodes represent target genes and yellow nodes represent miRNAs.Abbreviation: miRNA, microRNA.
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f1-ott-9-4121: A network of target miRNAs–messenger RNAs. Blue nodes represent target genes and yellow nodes represent miRNAs.Abbreviation: miRNA, microRNA.

Mentions: Predictive information from three databases, miRBase, miRanda, and Targets can be performed; overlapping parts of the three predictive results were served as final result predicted. Furthermore, relative functional analysis of target genes was made as well. Consequently, a large number of target genes mediated by miR-429 and relative functions were acquired. As illustrated in Figure 1, a large quantity of target genes was mediated by miR-429.


Effect of evodiamine and berberine on miR-429 as an oncogene in human colorectal cancer.

Liu H, Huang C, Wu L, Wen B - Onco Targets Ther (2016)

A network of target miRNAs–messenger RNAs. Blue nodes represent target genes and yellow nodes represent miRNAs.Abbreviation: miRNA, microRNA.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4940014&req=5

f1-ott-9-4121: A network of target miRNAs–messenger RNAs. Blue nodes represent target genes and yellow nodes represent miRNAs.Abbreviation: miRNA, microRNA.
Mentions: Predictive information from three databases, miRBase, miRanda, and Targets can be performed; overlapping parts of the three predictive results were served as final result predicted. Furthermore, relative functional analysis of target genes was made as well. Consequently, a large number of target genes mediated by miR-429 and relative functions were acquired. As illustrated in Figure 1, a large quantity of target genes was mediated by miR-429.

Bottom Line: Our results suggested that E-cadherin and Par3 were remarkably decreased in tumor tissues compared with those in normal tissues, and miR-429 was upregulated in tumor tissues.After treatment of BER and EVO, the level of miR-429 was lower in tumor tissues than in normal tissues.The data suggested that BER and EVO can be potential therapeutic agents for CRC, as they downregulated the expression level of miR-429.

View Article: PubMed Central - PubMed

Affiliation: Institute of Spleen and Stomach, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People's Republic of China.

ABSTRACT
Loss of epithelial organization and deregulated microRNAs are hallmarks of malignant carcinomas, but the relationship between them has been poorly understood. This study was designed to investigate the changes in the expression of E-cadherin, Par3, and miR-429 during the development of human colorectal cancer (CRC). E-cadherin and Par3 levels were quantitatively detected by immunohistochemistry and Western blotting. An in vitro culture of colorectal tissue was established to analyze the effect of berberine (BER) and evodiamine (EVO) on the level of miR-429. Our results suggested that E-cadherin and Par3 were remarkably decreased in tumor tissues compared with those in normal tissues, and miR-429 was upregulated in tumor tissues. After treatment of BER and EVO, the level of miR-429 was lower in tumor tissues than in normal tissues. This study investigated the potential relationship between miR-429, E-cadherin, and Par3 in CRC. The data suggested that BER and EVO can be potential therapeutic agents for CRC, as they downregulated the expression level of miR-429.

No MeSH data available.


Related in: MedlinePlus